Immobilized algae ball and preparation method and application thereof

A technology for algae balls and algae, which is applied to the field of immobilized algae balls and their preparation, can solve the problems of high mechanical strength of the immobilized algae balls, easy decomposition of the immobilized algae balls, and cannot be stored for a long time, and achieves convenient large-scale production, Good stability and activity, high stability effect

Active Publication Date: 2020-12-11
青岛鲜达物流科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the deficiencies of the above-mentioned technologies, and provide an immobilized algae ball and its preparation method and application. The immobilized algae ball prepared by the method has high mechanical streng

Method used

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  • Immobilized algae ball and preparation method and application thereof
  • Immobilized algae ball and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Cultivation of microbial algae liquid: inoculate Sclerophytum in BG11 medium, and cultivate for 8 days under the conditions of temperature 25-28°C, light intensity 5000±500Lx, and pH 6-7 to prepare microbial algae liquid.

Embodiment 2

[0033] Preparation of algae mixture: heat 8ml of acetyl tri-n-butyl citrate (ATBC) to 100°C and then cool down to 70-80°C for later use;

[0034] Add 2g of sodium alginate into 100ml of water, stir evenly at 60 to 70°C (sodium alginate is added in four times, 0.5g each time), sterilize with high temperature steam at 121°C for 20min, add chitosan 2g, stir well and then ultrasonically After ultrasonic treatment in the crusher for 1 min, add the cooled acetyl tri-n-butyl citrate, mix thoroughly and the temperature drops below 25°C.

[0035] Preparation of algae cross-linking agent: Add 25g of boric acid into 500ml of water, stir well to dissolve, add 5g of calcium chloride and continue to stir evenly, and adjust the pH to 7.0-7.4 with 2mol / L NAOH solution, and use it after high-temperature steam sterilization at 121°C.

[0036] Add the microbial algae solution prepared in Example 1 into the algae mixture at a volume ratio of 0.9:1, and stir evenly to obtain the embedding solution...

Embodiment 3

[0038] Preparation of algae mixture: heat 10ml of acetyl tri-n-butyl citrate (ATBC) to 100°C and then cool down to 70-80°C for later use;

[0039] Add 2.5g of sodium alginate into 100ml of water, stir evenly at 60 to 70°C (sodium alginate is added in four times), sterilize with high-temperature steam at 121°C for 20 minutes, add chitosan 3.5g, stir evenly, and place in an ultrasonic breaker After ultrasonic treatment for 1 min, add the cooled acetyl tri-n-butyl citrate, mix thoroughly and the temperature drops below 25°C.

[0040] Preparation of algae cross-linking agent: Add 25g of boric acid to 500ml of water, fully stir to dissolve, add 7.5g of calcium chloride and continue to stir evenly, and adjust the pH to 7.0-7.4 with 2mol / L NAOH solution, and use it after high-temperature steam sterilization at 121°C .

[0041] Add the microbial algae solution prepared in Example 1 into the algae mixture at a volume ratio of 1:1, and stir evenly to obtain the embedding solution; use ...

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Abstract

The invention relates to an immobilized algae ball and a preparation method and application thereof. The preparation method of the immobilized algae ball comprises the steps that firstly, a microbialalgae solution is cultured, then an algae mixed solution and an algae cross-linking agent are prepared, the microbial algae solution is added into the algae mixed solution to obtain an embedding solution, the embedding solution is dripped into the algae cross-linking agent by using a peristaltic pump, and the immobilized algae ball is obtained. Acetyl tri-n-butyl citrate (ATBC) and chitosan are added into the algae mixed solution, so that the obtained immobilized algae ball is higher in strength and not prone to damage, microalgae reproduction is faster, and the nitrogen and phosphorus treatment efficiency is higher. According to the prepared immobilized algae ball, only the ATBC and the chitosan are adopted as additives, a plurality of materials do not need to be used for improving the mechanical strength of the immobilized algae ball, the process is simplified, the good stability and activity of the immobilized algae ball are guaranteed, the ATBC and the chitosan are wide in source and low in price, the requirement for equipment is low, the toxicity to microorganisms is small, large-scale production is facilitated, and the immobilized algae ball is economical and efficient.

Description

technical field [0001] The invention relates to the technical field of aquaculture wastewater treatment, in particular to an immobilized algae ball and its preparation method and application. Background technique [0002] In recent years, my country's economic development is getting better and better, and people's living standards are gradually improving. In order to meet people's demand for seafood, the scale of my country's aquaculture industry is increasing every year, while seawater aquaculture has always used high-density and intensive farming methods. This kind of aquaculture The result of the method is that various pollutants in the water body, such as nitrogen and phosphorus, exceed the standard, and exceed the purification capacity of the water body itself, resulting in an imbalance in the breeding environment, which ultimately leads to a decrease in the survival rate of the culture and an increase in the cost of the culture. [0003] In order to solve the above-ment...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N11/04C02F3/32
CPCC12N11/10C12N11/04C02F3/322
Inventor 胡春义
Owner 青岛鲜达物流科技有限公司
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