Fluorescence imaging lighting device, imaging system and imaging method based on speckle principle

Abstract

The invention discloses a fluorescence imaging lighting device, an imaging system and an imaging method based on a speckle principle. The fluorescence imaging lighting device comprises a coupling optical fiber bundle and one or more mixed excitation light generation units. The imaging system comprises the fluorescence imaging lighting device based on the speckle principle, a mirror body, an imaging device and an acquisition control device. The imaging method comprises the following steps of controlling a fluorescence imaging illumination device of the system to alternately generate fluorescence excitation light for forming speckle illumination and fluorescence excitation light for forming uniform light illumination, controlling the imaging device to perform speckle illumination imaging anduniform light illumination imaging, and obtaining an optical chromatogram by adopting a HiLo optical tomography algorithm. On the basis of an existing wearable fluorescence microscopy system, a mixedcoherent light source and incoherent light source illumination method is adopted, an optical tomography image of a target sample is obtained, the imaging contrast ratio and resolution ratio are improved, and the application range of the wide-field fluorescence microscopy system is expanded.

Description

technical field [0001] The invention belongs to the field of biological imaging, and more specifically relates to a speckle-based fluorescent imaging lighting device, an imaging system and an imaging method. Background technique [0002] Imaging brain cells in conscious and freely moving animals is an important tool in the field of neuroscience to study the connection between animal behavior and neurons in the brain. During the corresponding behavior of animals, the action potential changes of neurons in specific areas of the brain are observed, so that the connection between animal behavior and neurons, circuits and higher-level neuron information processing can be studied. Therefore, the detection of neuronal activity signals is the basis of neuroscience research. Conventional methods such as electrophysiological recording have many problems such as high technical difficulty, low throughput, non-specificity, and lack of spatial resolution. The development of optical neura...

AI Technical Summary

Problems solved by technology

[0007] Aiming at the above defects or improvement needs of the prior art, the present invention provides a fluorescent imaging lighting device, imaging system and imaging method based on the speckle principle. The control of quality and quantity can improve the contrast of tomography while avoiding artifacts, so as to obtain higher-quality tomograms, thereby solving the problem of prior art images in miniature, wearable fluorescence microscopy imaging devices Technical issues with poor contrast

Images