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Application of perilla leaf extract to preparation of medicament for preventing and/or treating asthma

A technique of perilla leaf extract and asthma, which is applied in the field of perilla leaf extract and achieves the effects of simple preparation process, low toxicity and side effects, and prevention and treatment of asthma.

Pending Publication Date: 2020-12-15
INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no relevant research reports on perilla leaf extract and its treatment of asthmatic airway inflammation. In order to effectively explain the role of traditional Chinese medicine in treating asthma, and to provide theoretical basis and practical experience for searching for more active anti-asthmatic Chinese medicine, we prepared Perilla leaf extract was identified and its activity against airway inflammation in asthma was evaluated

Method used

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  • Application of perilla leaf extract to preparation of medicament for preventing and/or treating asthma
  • Application of perilla leaf extract to preparation of medicament for preventing and/or treating asthma
  • Application of perilla leaf extract to preparation of medicament for preventing and/or treating asthma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: the preparation of perilla leaf extract

[0042] Take 1.5 kg of dried perilla leaves, add 5 liters of 70% ethanol, heat to reflux for 1.5 hours, and filter while hot. The filter residue was treated once more in the same way; the filtrates were combined twice, and the ethanol was recovered under reduced pressure to obtain 65 g of extract. The extract was added with 300mL of water and stirred to form a suspension, which was extracted twice with 150mL of ethyl acetate respectively. Add 1580 mL of 95% ethanol to the remaining water to dissolve, precipitate at room temperature for 24 hours, filter under reduced pressure, recover ethanol from the filtrate under reduced pressure, freeze-dry and pulverize to obtain perilla leaf extract.

Embodiment 2

[0043] Embodiment 2: dosage form preparation

[0044] Capsules: Perilla leaf extract 100g, add dextrin 147.5g, magnesium stearate 2.5g, mix well, make 1000 capsules according to capsule preparation method, fill in 250mg capsules, each capsule contains perilla leaf extract 100mg , and its total amount is not less than 50%.

[0045] Tablets: Take 100g of perilla leaf extract, add 100g of starch, 80g of dextrin, and 15g of sucrose, stir well, make granules, dry below 60°C, add appropriate amount of magnesium stearate and talc, mix well, Compressed into 1000 tablets, coated with sugar or film, that is.

[0046] Granules: Take 100g of perilla leaf extract, add appropriate amount of sucrose and dextrin, make granules according to the granule preparation method, and dry to make 1000g.

[0047] Oral solution: Take perilla leaves, heat and reflux with 70% ethanol for 1.5 hours to extract twice, filter, combine the filtrate, recover ethanol under reduced pressure; add the same amount ...

experiment example 1

[0049] Experimental example 1: Inhibitory effect of perilla leaf extract on OVA-induced allergic asthma in mice

[0050] Methods: SPF grade male BALB / c mice, 18-20g, were randomly divided into normal control group, model control group, positive control group (dexamethasone, 0.5mg / kg, intragastric administration), drug treatment group (purple Perilla leaf extract 50, 100mg / kg, intragastric administration), 20 rats in each group. Animals in each group were injected intraperitoneally with a mixture of 100 μL (20 μg) of OVA solution and 100 μL of aluminum hydroxide gel on the first day of the experiment, and the blank control group was given an equal volume of normal saline; and on the 7th and 14th day after the first sensitization, respectively, Intraperitoneal injection of the same dose of OVA and aluminum hydroxide gel for immune boosting. After the animals were boosted on the 14th day, they were administered orally, once a day, for 14 consecutive days. Except that the blank ...

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Abstract

The invention discloses application of a perilla leaf extract to preparation of a medicament for treating and / or preventing asthma. The perilla leaf extract disclosed by the invention has a reversingeffect on the lung inflammation pathological injury of an asthma model mouse; generation of inflammatory cytokines of TNF-alpha, IL-1beta and the like in Bronchoalveolar Lavage Fluid (BALF) of the model mouse can be obviously inhibited; and the total number of leukocytes and the recruitment of lymphocytes and eosinophilic granulocyte in the BALF of the model mouse are obviously inhibited. The asthma effect of the model mouse is obviously improved.

Description

technical field [0001] The invention relates to a perilla leaf extract, a preparation method thereof, and an application thereof in preparation of medicine or food for preventing and / or treating asthma, belonging to the technical field of medicine or food. Background technique [0002] Bronchial asthma is one of the most common chronic respiratory diseases in the world today. According to the survey, there are about 300 million asthma patients in the world, and my country has about 30 million. The incidence rate is as high as 1-4%, which has seriously endangered the health of the people. The pathogenesis of asthma is complex, and multiple mechanisms such as airway inflammation, allergy, airway hyperresponsiveness, and airway remodeling participate in its pathological process, and airway inflammation is the basis of asthma and other respiratory diseases, and is the cause of recurrent asthma symptoms. The root cause of asthma plays an important and decisive role in the occurre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/535A61P11/06A61P37/08A61K127/00
CPCA61K36/535A61P11/06A61P37/08A61K2236/333A61K2236/39A61K2236/53A61K2236/51
Inventor 林明宝姚春所侯琦马培白金叶张梓倩吉腾飞范燕楠杨慧
Owner INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
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