Method for preparing white rot fungus liquid culture medium by using leather making waste and application

A liquid culture medium, white rot fungus technology, applied in microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of affecting microbial nutrient absorption, slow growth of microorganisms, etc. The effect of gentle reaction and full range of amino acids

Active Publication Date: 2020-12-18
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under static conditions, the agar will form a nutrient-permeable barrier around the microbial cells during the cultivation process, affecting the nutrient absorption of the microorganisms, resulting in slow growth of the microorganisms

Method used

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  • Method for preparing white rot fungus liquid culture medium by using leather making waste and application
  • Method for preparing white rot fungus liquid culture medium by using leather making waste and application
  • Method for preparing white rot fungus liquid culture medium by using leather making waste and application

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Effect test

Embodiment 1

[0037] A method for preparing white rot fungus liquid culture medium by utilizing tanning waste, comprising the following steps:

[0038] (1) Hydrolytic dechromation: In a constant temperature water bath at 60°C, add 25g of chromium leather scraps (containing Cr 2 o 3 : 3.2%), then add 3g of calcium oxide, 300g of deionized water, stir and react for 0.5h, add 12.5g of chrome leather shavings, continue to react for 0.5h, then add 12.5g of chrome leather shavings and continue to react for 1h to obtain a viscous The homogeneous mixture of hydrolyzed protein was removed by centrifugal suction while it was hot to remove insoluble solids, the filtrate was collected, the hydrolyzed protein sample obtained after dechromation was treated with carbon dioxide, the white precipitated calcium carbonate was removed by suction filtration, the filtrate was collected, and the filtrate was placed in a centrifuge Centrifuge at 8000r / min for 5 min, discard the precipitate, collect the liquid, an...

Embodiment 2

[0053] A method for preparing white rot fungus liquid culture medium by utilizing tanning waste, comprising the following steps:

[0054] (1) Hydrolytic dechromation: In a constant temperature water bath at 60°C, add 30g of chromium leather shavings (containing Cr 2 o 3 : 2.5%), then add 4.2g of calcium oxide, 390g of deionized water, stir and react for 0.5h, then add 15g of discarded chrome leather scraps, continue to react for 0.5h, then add 15g of chrome leather scraps and continue to react for 1h to obtain viscous The homogeneous mixture of hydrolyzed protein was removed by centrifugal suction while it was hot to remove insoluble solids, the filtrate was collected, the hydrolyzed protein sample obtained after dechromation was treated with carbon dioxide, the white precipitated calcium carbonate was removed by suction filtration, the filtrate was collected, and the filtrate was placed in a centrifuge Centrifuge at 8000r / min for 5 min, discard the precipitate, collect the l...

Embodiment 3

[0069] A method for preparing white rot fungus liquid culture medium by utilizing tanning waste, comprising the following steps:

[0070] (1) Hydrolytic dechromation: In a constant temperature water bath at 80°C, add 20g of chromium leather scraps (containing Cr 2 o 3 : 5%), then add 3.6g of calcium oxide, 400g of deionized water, stir and react for 0.5h, then add 10g of chrome leather shavings, continue to react for 0.5h, then add 10g of chrome leather shavings and continue to react for 1h to obtain viscous The hydrolyzed protein homogeneous mixture was centrifugally filtered while it was hot to remove the insoluble solid matter, and the filtrate was collected. The hydrolyzed protein sample obtained after dechromation was treated with carbon dioxide, and the white precipitated calcium carbonate was removed by suction filtration, and the filtrate was collected, and the filtrate was placed in a centrifuge Centrifuge at 8000r / min for 5 min, discard the precipitate, collect the ...

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Abstract

The invention relates to a method for preparing a white rot fungus liquid culture medium by using leather making waste and application. The method comprises the following steps: firstly, taking leather making waste chrome leather scraps as raw materials, preparing dechromized hydrolyzed collagen by adopting a batch feeding process based on an alkali-enzyme hydrolysis method, and preparing a leaching solution by using the leather making waste sawdust; secondly, taking the extracted dechromized hydrolyzed collagen and the sawdust leaching solution as nutrient sources, and compounding glucose, peptone, a trace element solution and purified water to prepare a liquid fermentation culture medium; and finally, culturing white rot fungi by using the liquid fermentation culture medium. According tothe liquid culture medium prepared by the method, normal and efficient growth of the white rot fungi can be realized, the purpose of turning waste into wealth of the leather making waste chrome leather scraps and the sawdust is achieved, a traditional peptone resource can be greatly replaced, the culture cost of the white rot fungi is effectively reduced, and high-value utilization of chromium-containing solid waste is facilitated.

Description

technical field [0001] The invention belongs to the field of leather solid waste, and in particular relates to a method and application for preparing white rot fungus liquid culture medium from tanning waste. Background technique [0002] In recent years, with the improvement of living standards, people's demand for various, beautiful and practical leather products has become more and more vigorous, which has also led to the rapid development of my country's leather industry in the past 30 years. Nowadays, leather products such as suitcases, leather shoes, leather bags, leather sofas, leather chairs, etc., which are beautiful and individual, have long been inseparable from our lives. The traditional tanning process, from a macro perspective, is the process of transforming the animal raw materials discarded by animal husbandry into leather products with high use value and high artistic value. It is a recycling method of natural resources. It is the existence of the downstrea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P21/06C07K14/78C12R1/645
CPCC12N1/14C12P21/06C07K14/78
Inventor 刘洁张斐斐徐洲李彦春李雪松周玉
Owner QILU UNIV OF TECH
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