Starch-based dextrin modified zein nanoparticles as well as preparation method and application thereof
A zein and nanoparticle technology, applied in powder delivery, organic active ingredients, cosmetic preparations, etc., can solve problems such as application limitation, multiple emulsion thermodynamic instability, etc., and achieve simple preparation method, easy industrial production, strong strength. The effect of antioxidant properties
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[0029] One aspect of the embodiments of the present invention provides a method for preparing starch-based dextrin-modified zein nanoparticles, comprising:
[0030] The first mixed reaction system containing zein, tea polyphenol ester, sodium hydroxide, ethanol and water was reacted at 25° C. for 24 h to obtain a zein-tea polyphenol ester covalent complex, and then the covalent complex containing corn The second mixed reaction system of prolamin-tea polyphenol ester covalent complex, sodium carbonate, ethanol and water was reacted at 25°C for 4 hours to obtain hollow zein-tea polyphenol ester nanoparticles;
[0031] Co-precipitation reaction occurs in the third mixed reaction system comprising carboxymethyl dextrin, tea polyphenol ester, ethanol and water to obtain carboxymethyl dextrin-tea polyphenol ester covalent complex;
[0032] And, the fourth mixed reaction system comprising the hollow zein-tea polyphenol ester nanoparticles, the carboxymethyl dextrin-tea polyphenol est...
Embodiment 1
[0105] (1) Preparation of hollow zein-tea polyphenol ester nanoparticles
[0106]Dissolve zein and tea polyphenol ester in 80% (v / v) ethanol solution respectively, adjust the pH value to 9.0 with 0.1mol / L sodium hydroxide solution after mixing, and stir continuously at 25°C The reaction was carried out for 24 hours. After the reaction was completed, the obtained mixed solution was ultrasonically dialyzed in a water bath for 24 hours, during which the dialysate was replaced 8 times to remove free tea polyphenol esters. Finally, the solution was freeze-dried to obtain zein-tea Polyphenol ester covalent complexes;
[0107] Dissolve 2 g of zein-tea polyphenol ester covalent complex in 100 mL of ethanol solution (80% by volume), stir at 800 rpm for 2 h, and let stand to dissolve completely. Water ethanol is uniformly mixed by volume ratio 3:7 to form an ethanol suspension of sodium carbonate, the above suspension is mixed with zein-tea polyphenol ester covalent complex solution by...
Embodiment 2
[0121] (1) Preparation of hollow zein-tea polyphenol ester nanoparticles
[0122] Dissolve zein and tea polyphenol ester in 80% (v / v) ethanol solution respectively, adjust the pH value to 9.0 with 0.1mol / L sodium hydroxide solution after mixing, and stir continuously at 25°C The reaction was carried out for 24 hours. After the reaction was completed, the obtained mixed solution was ultrasonically dialyzed in a water bath for 24 hours, during which the dialysate was replaced 8 times to remove free tea polyphenol esters. Finally, the solution was freeze-dried to obtain zein-tea Polyphenol ester covalent complexes;
[0123] Dissolve 2 g of zein-tea polyphenol ester covalent complex in 100 mL of ethanol solution (80% by volume), stir at 800 rpm for 2 h, and let stand to dissolve completely. Water ethanol is uniformly mixed by volume ratio 3:7 to form an ethanol suspension of sodium carbonate, the above suspension is mixed with zein-tea polyphenol ester covalent complex solution b...
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