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Electrochemical luminescence aptamer sensor for detecting kanamycin and preparation method of electrochemical luminescence aptamer sensor

An aptamer sensor, kanamycin technology, applied in chemiluminescence/bioluminescence, material electrochemical variables, scientific instruments, etc., can solve the problems of complex experimental operation, large result error, long measurement time, etc. The effect of good performance, enhanced signal strength, and low detection cost

Active Publication Date: 2020-12-29
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, the commonly used methods for detecting kanamycin mainly include high performance liquid chromatography, enzyme-linked immunosorbent assay, liquid chromatography, gas chromatography, electrochemical method, etc., but these methods generally have complicated experimental operations. And time-consuming and other shortcomings, it is difficult to promote to the market for on-site testing
[0004] Existing aptamer sensors have high detection sensitivity, but the detection cost is high, the measurement time is long, and the result error is large

Method used

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  • Electrochemical luminescence aptamer sensor for detecting kanamycin and preparation method of electrochemical luminescence aptamer sensor
  • Electrochemical luminescence aptamer sensor for detecting kanamycin and preparation method of electrochemical luminescence aptamer sensor
  • Electrochemical luminescence aptamer sensor for detecting kanamycin and preparation method of electrochemical luminescence aptamer sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] (1) Assembly of an electrochemiluminescence aptasensor for the detection of kanamycin

[0054] (1) Preparation of PTC-Cys and Au@HKUST-1 materials:

[0055] S1. Preparation of precursor PTCA, dissolve 0.1g PTCDA in 20mL aqueous solution containing 0.04g NaOH, then slowly add 1M HCl solution to the above solution until a bright red precipitate is formed, wash the precipitate with deionized water Several times, until the pH value of the suspension is about 7, dry it for later use. Accurately weigh 5 mg of the above-prepared PTCA, disperse it in 10 mL of deionized water, and ultrasonically disperse evenly, stir in an ice-water bath for 30 min, add 40 mg of EDC and 10 mg of NHS (m / m=4:1), and continue stirring for 2 h to Activate the carboxyl group, then add 100 mg of cysteine, stir overnight, and finally, collect the product by centrifugation as PTC-Cys; disperse PTC-Cys in DMF to make it evenly dispersed, and obtain PTC-Cys with a concentration of 1 mg / mL DMF dispersion...

Embodiment 2

[0071] (1) Assembling the electrochemiluminescence aptasensor for detecting kanamycin in Example 1

[0072] Step (1) During the preparation of the PTC-Cys material, in the amidation reaction of S1, the ratio of EDC to NHS was changed to 1:1 (m / m), and the others were the same as in Example 1.

Embodiment 3

[0074] In Example 1 (1) assembling an electrochemiluminescent aptasensor for detecting kanamycin, in step (2), the concentration of the aptamer in the aptamer solution is 2-10 μM.

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Abstract

The invention belongs to the field of electrochemical luminescence detection, and particularly relates to an electrochemical luminescence aptamer sensor for detecting kanamycin and a preparation method of the electrochemical luminescence aptamer sensor. An aptamer is loaded on the surface of a composite material Au@HKUST 1 / PTC Cys modified glassy carbon electrode, the surface of the glassy carbonelectrode is jointly modified by utilizing Au-S bond combination between Au@HKUST 1 and PTC Cys, so that the sensitivity and stability of electrochemical luminescence are remarkably improved; and thenthe aptamer is loaded to obtain the electrochemical luminescence aptamer sensor, so that the target molecule kanamycin can be specifically recognized, and the selectivity of kanamycin detection is improved. According to the electrochemical luminescence aptamer sensor, the detection range is 1.0 x 10 < -13 > - 1.0 x 10 < -8 > M, and the lowest detection limit is 4.2 x 10 < -14 > M; and the preparation method of the modified electrode is simple, the sensitivity of kanamycin detection is high, the selectivity is good, and the linear range is wide.

Description

technical field [0001] The invention belongs to the field of electrochemiluminescence detection, and in particular relates to an electrochemiluminescence aptamer sensor for detecting kanamycin and a preparation method thereof. Background technique [0002] Food safety issues have seriously affected people's lives. It is very important to monitor food safety. Kanamycin (KAN), an aminoglycoside antibiotic, is potentially toxic, and excessive use of KAN can cause serious side effects in humans and animals, including ototoxicity, nephrotoxicity, and anaphylactic shock, affecting consumers health. In order to improve people's awareness of the hazards of antibiotic residues and effectively protect human health, the European Union has clearly stipulated that the maximum residue limit (MRL) of kanamycin in milk is 150 μg kg -1 , due to the unreasonable use of kanamycin will lead to residues in meat, dairy products and other animal-derived foods, these residues will enter the huma...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/30G01N21/76
CPCG01N27/3278G01N27/308G01N21/76
Inventor 陈智栋周利君单学凌蒋鼎王文昌
Owner CHANGZHOU UNIV