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A detection method for simultaneous determination of multiple active components in lung-clearing medicines

A determination method and technology for active ingredients, applied in the field of medicine, can solve the problems of single, inability to monitor the quality of prescriptions, and the detection of active ingredients is cumbersome, and achieve the effect of high precision and wide range of use.

Active Publication Date: 2022-08-02
ZHEJIANG PROVINCIAL PEOPLES HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, the detection of the active ingredients of various drugs in Qingfei formula is cumbersome and single, and it is impossible to comprehensively control and monitor the quality of the corresponding formula

Method used

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  • A detection method for simultaneous determination of multiple active components in lung-clearing medicines
  • A detection method for simultaneous determination of multiple active components in lung-clearing medicines
  • A detection method for simultaneous determination of multiple active components in lung-clearing medicines

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Embodiment 1

[0050] Example 1: Establish a method for determining the content of chlorogenic acid, caffeic acid, p-coumaric acid, scutellarin, rosmarinic acid, and apigenin (active components are chlorogenic acid, caffeic acid, p-coumaric acid, scutellarin fingerprints of glycosides, rosmarinic acid and apigenin)

[0051] 1.1 Preparation of reference stock solution

[0052] Precisely weighed chlorogenic acid and caffeic acid reference substances were 4.37 mg and 5.90 mg, respectively, and placed them in a 5ml volumetric flask. 5.71, 4.67, and 2.38 mg, respectively, were placed in 10 ml measuring bottles, dissolved in methanol and diluted to the mark, and shaken to obtain the concentrations of 0.874 mg / ml, 1.18 mg / ml, 0.626 mg / ml, and 0.571 mg / ml, respectively. , 0.467mg / ml, 0.238mg / ml of chlorogenic acid, caffeic acid, coumaric acid, scutellarin, rosmarinic acid, apigenin reference stock solution. Store in a refrigerator at 4°C for later use.

[0053] 1.2 Preparation of the test solutio...

Embodiment 2

[0091] Embodiment 2. Fritillin A, Fritillin B content assay method

[0092] Chromatographic conditions: Column: Kromasil C18, mobile phase: acetonitrile-water-diethylamine (70:30:0.03), flow rate: 0.7ml / min, column temperature: 30℃, evaporative light detector, drift tube temperature: 45 ℃, nitrogen flow rate 1.5L / min. Two-point determination method of external standard: Precisely draw 10 μl and 20 μl of the reference solution and 10 μl of the test solution, respectively, and inject them into the liquid chromatograph for determination. The contents of fritillin A and fritillin B were calculated by logarithmic equation. Preparation of the test solution: Rotary evaporation of the test solution in 1.2 at 80°C to 90ml to obtain a concentrated medicinal solution containing 2.5g crude drug per ml, accurately draw 10ml of the concentrated medicinal solution, place it in a flask, and add 0.8 of the concentrated ammonia test solution. ml soaked for 1.5 hours, accurately added 40ml of ...

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Abstract

The invention discloses an HPLC method for simultaneously determining the contents of chlorogenic acid, caffeic acid, p-coumaric acid, scutellarin, rosmarinic acid and apigenin in lung-clearing medicines, and a method for detecting fritillin A and fritillin B. Methods The fingerprints of the active ingredients of medicines were established, which ensured the precision, stability, repeatability, specificity and accuracy of the measurement method.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to a detection method for simultaneously measuring the active components of multiple lung-clearing medicines, and a method for establishing an HPLC standard fingerprint of the active components. Background technique [0002] The Qingfei recipe broke the cocoon in "Jingyue Quanshu", which was recorded in the "Jingyue Quanshu". It is mainly used for cough and phlegm, chest pain and hemoptysis caused by lung cancer, pneumonia and radiation pneumonia. After years of improvement and development, Qingfei Prescription has derived a variety of drug combinations for different symptoms. Since 2002, the quality control research, pharmacological efficacy research, and clinical research of Qingfei Mixture have continued to develop and have made certain progress. [0003] The fingerprint of traditional Chinese medicine refers to the chromatogram or spectrogram that can indicate the chemical characteristics...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/8686G01N2030/047
Inventor 黄萍张轶雯朱俊峰刘宇佳潘宗富盛孔胜
Owner ZHEJIANG PROVINCIAL PEOPLES HOSPITAL