Method for extracting astaxanthin from shrimp shells by using amino acid natural eutectic solvent

A low eutectic solvent and amino acid technology, applied in organic chemistry and other directions, can solve problems such as toxicity, achieve the effects of low cost, suitable for industrial scale production, and high extraction efficiency

Pending Publication Date: 2021-01-01
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In general, most traditional organic solvents are highly volatile, potentially toxic to the human environment, and are banned for use in products for human consumption

Method used

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  • Method for extracting astaxanthin from shrimp shells by using amino acid natural eutectic solvent
  • Method for extracting astaxanthin from shrimp shells by using amino acid natural eutectic solvent
  • Method for extracting astaxanthin from shrimp shells by using amino acid natural eutectic solvent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Mix L-lysine and glycerol at a molar ratio of 1:2, and perform magnetic stirring at 80°C until a transparent and uniform liquid is formed, and finally a natural deep eutectic solvent is obtained, which is sealed and stored.

[0043] The shrimp shells are dried, crushed, and then placed in a pulverizer for crushing, and then passed through a 40-mesh sieve to obtain shrimp shell powder, which is sealed and set aside. Weigh 1.5g of crushed shrimp shells, put them into a round bottom flask, add 25mL of 0.5mol / L HCL solution, and stir for 1 hour in the dark for decalcification treatment. After acid treatment for a specified time, filter and wash, and dry the filter cake . Accurately weigh 0.5 g of decalcified shrimp shell powder, add 5 mL of deep eutectic solvent (water content 30%), vortex for 1 min, ultrasonically extract for 30 min, power 200w, temperature 30°C. Centrifuge at 4200r / min for 15min, and take the supernatant, which is the shrimp shell astaxanthin extract. T...

Embodiment 2

[0045]The difference from Example 1 is that the hydrogen bond acceptor of the natural deep eutectic solvent is L-lysine, and the hydrogen bond donor is xylitol.

Embodiment 3

[0047] The difference from Example 1 is that the hydrogen bond acceptor of the natural deep eutectic solvent is L-lysine, and the hydrogen bond donor is citric acid.

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Abstract

The invention belongs to the technical field of natural product extraction, and particularly relates to a method for extracting astaxanthin in shrimp shells by using an amino acid natural eutectic solvent. The amino acid natural eutectic solvent is obtained by mixing and heating a hydrogen bond donor and a hydrogen bond acceptor, the molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1: 1-1: 10, and the hydrogen bond donor comprises citric acid, glycerol and xylitol; and the hydrogen bond receptor comprises Lproline and Llysine. The extraction method comprises the following steps: drying, crushing and sieving shrimp shells, and treating the screened shrimp shell powder with hydrochloric acid to obtain decalcified shrimp shells; and mixing the decalcified shrimp shellswith an amino acid eutectic solvent, adding water, carrying out ultrasonic treatment, centrifuging, and taking the supernatant, thereby obtaining the astaxanthin extract from the shrimp shells. According to the method, the astaxanthin in the shrimp shells is extracted, and the purposes of high extraction efficiency, no organic solvent pollution, low toxicity, environmental friendliness and high product purity are achieved.

Description

【Technical field】 [0001] The invention belongs to the technical field of natural product extraction, and in particular relates to a method for extracting astaxanthin in shrimp shells with an amino acid natural deep eutectic solvent. 【Background technique】 [0002] Astaxanthin is a non-vitamin A-derived ketolipid-soluble carotene that is dark pink in color. Widely present in the biological world, especially in shrimp, crab, fish, algae, yeast and bird feathers, it is one of the main carotenoids in marine organisms. Astaxanthin has extremely strong antioxidant properties due to its long conjugated double bond chain and unsaturated α-hydroxy ketone in its molecule. Compared with lutein, vitamin C, β-carotene, etc., the presence of conjugated double bonds, hydroxyl groups and ketone groups makes astaxanthin both hydrophilic and hydrophobic, and can pass through the hydrophobic interior of the cell membrane to reach the hydrophilic Sexual external space, and has better biologic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24
CPCC07C403/24C07C2601/16
Inventor 赵龙山邓美凤
Owner SHENYANG PHARMA UNIVERSITY
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