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Lactobacillus plantarum, microbial agent and application thereof

A technology of Lactobacillus plantarum and microbial inoculum, which is applied in the field of microorganisms to achieve the effects of delaying skin aging, strong anti-oxidation, and reducing acne

Inactive Publication Date: 2021-01-01
深圳市沁帆科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Usually beauty lovers use botulinum toxin injections, collagen subcutaneous injections, skin tightening surgery and other surgical methods to delay skin aging, which is very risky

Method used

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  • Lactobacillus plantarum, microbial agent and application thereof
  • Lactobacillus plantarum, microbial agent and application thereof
  • Lactobacillus plantarum, microbial agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Isolation and identification of Lactobacillus plantarum GDMCC No: 61028

[0019] Separated from kimchi, crushed, serially diluted 10, 100, 1000, 10000, and spread 100 μL on the MRS solid medium containing 1% vancomycin, cultured upside down at 37°C for 24 hours, picked a single colony and marked Lines were placed on MRS solid medium containing 1% vancomycin, cultured upside down at 37°C for 24 hours, single colonies were picked, colony PCR was performed, and the PCR results were sequenced and compared.

[0020] The 16S rRNA base sequence of the isolated strain was analyzed and sequenced, and the sequence is as follows:

[0021]

[0022] That is, SEQ ID NO:1.

[0023] Through the above identification results, it is confirmed that the isolated strain belongs to plant Lactobacillus, Lactobacillus plantarumsubsp.plantarum, the bacteria are short rod-shaped Gram-positive bacteria, the colonies are milky white, smooth, and the edges are neat. They are preserv...

Embodiment 2

[0024] Example 2: Effect of Lactobacillus plantarum GDMCC61028 on the water content of aging mouse skin

[0025] Lactobacillus plantarum GDMCC No: 61028 can significantly maintain the moisture content of aging mouse skin. Give 17-month-old mice daily gavage of 10 9 CFU / mL Lactobacillus plantarum GDMCC No: 61028 fermented milk 100μL, after 3 months, that is, 20-month-old mice, use the skin moisture tester to measure the skin moisture content, and found that the skin moisture content reached 22.95%, which is the same as that of 4-month-old mice There is basically no significant difference in the water content of the mouse skin at 23.39%, but it is significantly increased compared with the 20-month old mouse (20.69%), the results are shown in figure 1 .

Embodiment 3

[0026] Embodiment 3: Effect of Lactobacillus plantarum GDMCC61028 on the collagen content of aging mouse skin

[0027] Lactobacillus plantarum GDMCC No: 61028 can significantly maintain the collagen content of aging mouse skin. Give 17-month-old mice daily gavage of 10 9 CFU / mL of Lactobacillus plantarum GDMCC No: 61028 fermented milk 100μL, after 3 months, skin samples were taken for collagen staining, and 20-month-old mice were used as controls, the results were as follows figure 2 and image 3 shown. image 3 It was shown that the content of collagen increased significantly after gavage of Lactobacillus plantarum.

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Abstract

The invention discloses lactobacillus plantarum. The strain name of the lactobacillus plantarum is Lactobacillus plantarum subsp. Plantarum FLPL135, thalli of the strain are short-rod-shaped gram-positive bacteria, bacterial colonies of the strain are milk white, smooth in surface and neat in edge, and the strain is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 61028. The invention belongs to the technical field of microorganisms, and provides lactobacillus plantarum GDMCC No: 61028 which has very strong oxidation resistance, can significantly improve the content of collagen in aging skin, and has a significant inhibition function on bacillus acnes.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a new Lactobacillus plantarum subsp. plantarum (GDMCC No: 61028), a microbial agent and an application thereof. Background technique [0002] Lactobacillus plantarum belongs to a kind of lactic acid bacteria. It is Gram-positive and does not produce spores. It is anaerobic or facultative anaerobic bacteria. Different type of lactic acid bacteria can ferment gluconate or pentose sugar, lactic acid is the most fermented end product, reaching more than 85%, and can produce unique lactobacillus during the reproduction process. Lactobacillus plantarum has good adaptability and can survive in a variety of environments. Different strains have different living habits, and it is the only microorganism that can survive in a variety of ecological environments. Comparative genomics analysis found that Lactobacillus plantarum has a nomadic lifestyle in nature. The adaptability of the b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/123A23L19/00A23L2/38A23L2/52A23L33/135A61K35/747A61K8/99A61Q19/08A61P39/06C12R1/25
CPCC12N1/20A23C9/1234A23L19/00A23L2/382A23L2/52A23L33/135A61K35/747A61K8/99A61Q19/08A61P39/06A23V2002/00C12R2001/25C12N1/205A23V2400/169A23V2200/318
Inventor 徐锋蔚晓敏武晓丽陈沁
Owner 深圳市沁帆科技有限公司
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