Non-enzymatic electrochemical sensor for detecting glutamic acid concentration and detection method adopting non-enzymatic electrochemical sensor

A detection method, glutamic acid technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problem that non-enzyme sensors cannot be applied to biological sample detection, and achieve the effect of a shortcut method

Pending Publication Date: 2021-01-08
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, these non-enzymatic sensors cannot be applied to the detection of biological samples

Method used

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  • Non-enzymatic electrochemical sensor for detecting glutamic acid concentration and detection method adopting non-enzymatic electrochemical sensor
  • Non-enzymatic electrochemical sensor for detecting glutamic acid concentration and detection method adopting non-enzymatic electrochemical sensor
  • Non-enzymatic electrochemical sensor for detecting glutamic acid concentration and detection method adopting non-enzymatic electrochemical sensor

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Experimental program
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Embodiment 1

[0065] This embodiment provides a non-enzyme electrochemical sensor for detecting glutamic acid, the electrochemical sensor such as figure 1 As shown, the reaction cell 4 is included, and the working electrode 1, the auxiliary electrode 2 and the reference electrode 3 are arranged in the reaction cell 4; the three-electrode system is constructed by the working electrode 1, the auxiliary electrode 2 and the reference electrode 3, and the reaction cell Glutamic acid in the solution to be tested in 4 is carried out trace detection. The working electrode 1 is a carbon-based electrode whose surface is modified with nickel oxide-reduced graphene oxide nanomaterials, and the nickel oxide in the nickel oxide-reduced graphene oxide nanomaterials includes nickel oxide and nickel oxide. The reference electrode 3 is a carbon-based electrode, and the auxiliary electrode 2 is an Ag / AgCl electrode.

[0066] The nickel oxide-reduced graphene oxide nanomaterial is modified to the surface of t...

Embodiment 2

[0071] The non-enzymatic electrochemical sensor provided in Example 1 is used to carry out the method for trace detection of glutamic acid in the solution to be tested, and the detection method specifically includes the following steps:

[0072] (1) Using a non-enzyme electrochemical sensor to carry out cyclic voltammetry detection on 7 groups of base fluids with known glutamic acid concentration, during the cyclic voltammetry detection process, glutamic acid in the working electrode of the non-enzyme electrochemical sensor 1 Catalytic oxidation reaction occurs on the surface, so that the oxidation peak of glutamic acid appears in the voltage range of 0.4 ~ 0.55V, the base fluid is alkaline phosphate buffer solution containing glutamic acid, the pH of the base fluid is 13, set the cycle voltage The scanning voltage range of Anfa is 0~1.0V, and the scanning speed is 0.1V / s;

[0073] The concentration of glutamic acid in the base fluid with known glutamic acid concentration in t...

Embodiment 3

[0080] The non-enzymatic electrochemical sensor provided in Example 1 is used for the method of trace detection of glutamic acid in the test solution containing interfering substances, wherein the interfering substances in the test solution include 20 μM uric acid, 100 μM ascorbic acid and 2 mM glucose. Described detection method specifically comprises the following steps:

[0081] (1) Using a non-enzyme electrochemical sensor to carry out cyclic voltammetry detection on 7 groups of base fluids with known glutamic acid concentration, during the cyclic voltammetry detection process, glutamic acid in the working electrode of the non-enzyme electrochemical sensor 1 Catalytic oxidation reaction occurs on the surface, so that the oxidation peak of glutamic acid appears in the voltage range of 0.4 ~ 0.55V, the base fluid is alkaline phosphate buffer solution containing glutamic acid, the pH of the base fluid is 13, set the cycle voltage The scanning voltage range of Anfa is 0~1.0V, ...

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Abstract

The invention provides a non-enzymatic electrochemical sensor for detecting glutamic acid concentration and a detection method adopting the non-enzymatic electrochemical sensor. The non-enzymatic electrochemical sensor comprises a reaction tank, and a working electrode, an auxiliary electrode and a reference electrode which are arranged in the reaction tank. A three-electrode system is constructedthrough the working electrode, the auxiliary electrode and the reference electrode, and trace detection is carried out on glutamic acid in a to-be-detected solution in the reaction tank; and the working electrode is a carbon-based electrode of which the surface is modified with a nickel oxide reduced graphene oxide nano material. According to the invention, low-concentration glutamic acid in a sample is detected in a non-enzymatic electrochemical sensing manner, and relatively high current response to glutamic acid concentration in a range of 0.1 nM to 1 [mu]M can be realized, so that the purpose of trace detection of the glutamic acid is realized, and a very cheap and rapid method is provided for the trace detection of glutamic acid in the field of biological and medical application. Themethod is worthy of popularization.

Description

technical field [0001] The invention belongs to the technical field of trace detection, relates to a non-enzyme electrochemical sensor and a detection method using the same, in particular to a non-enzyme electrochemical sensor for detecting glutamic acid concentration and a detection method using the same. Background technique [0002] Glutamate is the main excitatory neurotransmitter in the mammalian central nervous system, and plays an important role in life activities such as learning and memory and neurodevelopment. Under physiological conditions, the concentration and distribution of glutamate in the brain are strictly regulated to maintain homeostasis. Under pathological conditions, especially in the pathogenesis of some serious neurological diseases such as stroke, neurodegenerative diseases, schizophrenia, epilepsy, etc., due to the inhibition of the reuptake of glutamate transporters, the extracellular Glutamate accumulates in large quantities, which can rise from ...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/30G01N27/327C25D5/54C25D9/04
CPCG01N27/48G01N27/308G01N27/3278C25D9/04C25D5/54
Inventor 赵帅男孙坚原申雪峰陈培华
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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