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Fresh grapefruit pulp aqueous extract powder, as well as preparation, antithrombotic effect and application thereof

A technology for water extract and pomelo meat is applied in the application fields of preparing fresh pomelo meat water extract powder, preparing anti-arterial thrombosis drugs and anti-venous thrombosis drugs, and can solve the problems of no substantial progress and the like

Inactive Publication Date: 2021-01-15
北京恒润泰生医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, no substantive progress has been made

Method used

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  • Fresh grapefruit pulp aqueous extract powder, as well as preparation, antithrombotic effect and application thereof
  • Fresh grapefruit pulp aqueous extract powder, as well as preparation, antithrombotic effect and application thereof
  • Fresh grapefruit pulp aqueous extract powder, as well as preparation, antithrombotic effect and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1 prepares fresh pomelo meat aqueous extract powder

[0040] Wash 120g pomelo meat twice with 300mL three-distilled water, then cut into small pieces. Mix small pieces of pomelo meat with triple-distilled water and soak at 60°C-90°C (100mL×3, 30 minutes each time). The obtained 320mL yellow liquid was concentrated under reduced pressure to obtain 30g syrup-like powder.

Embodiment 2

[0041] Example 2 Determination of chromatographic and mass spectrometry ion chromatograms of fresh pomelo meat aqueous extract powder

[0042] 2-1. Preparation of sample solution (10mg / mL)

[0043] Weigh 26.7mg fresh pomelo meat aqueous extract powder, and dissolve the powder in 2.67mL ultrapure water. The obtained solution was ultrasonically shaken for 1 minute, and then centrifuged at 13000r / min for 10 minutes. Take the supernatant, pass through a 0.22μm filter membrane, and place it in a sample bottle for chromatography and mass spectrometry.

[0044] 2-2. Chromatographic conditions

[0045] Column: Waters, Acquity T3 column (2.1×100mm i.d., 1.7μm); Injection volume: 2μL; PDA detector: 190-400nm; Mobile phase: water (0.1% formic acid), acetonitrile; Use this mobile phase and wash according to the gradient of Table 1 column.

[0046] Table 1 Mobile phase gradient

[0047]

[0048]

[0049] 2-3. Conditions for measuring ion current

[0050] Electrospray ionizati...

Embodiment 3

[0053] Embodiment 3 specifies the structure of 25 components in the fresh pomelo meat aqueous extract powder

[0054] The UPLC chromatography of Example 2 was coupled with mass spectrometry, and the UPLC-mass spectrometry of fresh pomelo meat aqueous extract powder was determined. The mass spectrometry conditions are two modes of electrospray ionization, positive ion and negative ion. The slope well collision energy is 20-60V. The ion source temperature was 120°C. The pressure of the nebulizer is 6.5 bar. The mass / charge ratio ranges from 100-1500. 25 independent peaks resolved within 47 minutes. According to the fragmentation law of mass spectrometry, these peaks (the peaks of the total ion current spectrum are numbered sequentially from left to right) are designated as the corresponding components in Table 2. See Figure 2 to Figure 26 .

[0055] Table 2 Retention time, mass number, structure and name of the components corresponding to the peaks in the total ion curre...

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PUM

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Abstract

The invention discloses fresh grapefruit pulp aqueous extract powder, a preparation method thereof and an application of the fresh grapefruit pulp aqueous extract powder to the aspect of resisting venous thrombosis and arterial thrombosis. The fresh grapefruit pulp aqueous extract powder prepared by the method provided by the invention contains 15 quinic acid compounds and 10 flavonoid compounds.Experiments prove that the fresh grapefruit pulp aqueous extract powder has good effect of resisting venous thrombosis and arterial thrombosis, so that the fresh grapefruit pulp aqueous extract powderprovides an effective technical means for treating venous thrombosis and arterial thrombosis.

Description

technical field [0001] The invention relates to a method for preparing water extract powder of fresh pomelo flesh, and relates to the application of the water extract powder of fresh pomelo flesh prepared by the method in the preparation of anti-arterial thrombosis drugs and anti-venous thrombosis drugs. The invention belongs to the field of biomedicine. Background technique [0002] Venous thrombosis has become one of the diseases with high morbidity and mortality. The most serious venous thrombosis is mainly cerebral venous thrombosis and lower extremity deep venous thrombosis. Cerebral venous thrombosis originates from dural venous sinus thrombosis and / or cerebral venous texture thrombosis, which can lead to cerebral venous blockage, increased intracranial pressure, cerebral ischemia or intracranial hemorrhage. The standard treatment strategy for cerebral venous thrombosis is oral anticoagulation. The incidence of upper / lower extremity deep vein thrombosis and pulmonar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/752A61K9/14A61P7/02A61K131/00
CPCA61K9/14A61K36/752A61K2236/331A61P7/02
Inventor 彭师奇赵明黄飞
Owner 北京恒润泰生医药科技有限公司
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