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Preparation method of enzymatic grafting based antioxidant silk fibroin membrane

A technology of oxidizing silk and silk fibroin, which is applied in the preparation of anti-oxidative silk film, flavonoids grafted on the surface of silk fibroin to prepare anti-oxidative silk film. The treatment conditions are relaxed and the performance is obvious

Active Publication Date: 2014-05-28
广东资恩解码基因检测科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, protease is mostly used for the degradation of silk fibroin; transglutaminase can catalyze the grafting of glutamic acid in silk fibroin to adjacent silk peptides or exogenous amino compounds, but due to the low glutamic acid content in silk fibroin, this Enzymes are rarely used in biomodification of silk fibroin materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Preparation of silk fibroin solution: add the degummed silkworm silk into lithium bromide-ethanol-water (mass ratio 45:45:10) solution, and dissolve at 60°C to prepare a 50g / L silk fibroin solution. Put the silk fibroin solution into a dialysis bag, dialyze in deionized water for 36 hours, change the water every hour during this period, and filter to obtain silk fibroin aqueous solution after dialysis.

[0025] (2) Preparation of silk fibroin film: Pour a certain amount of silk fibroin solution into a polytetrafluoroethylene (PTFE) mold, form a film by casting, and fully dry at -50°C to form a film.

[0026] (3) Enzymatic silk fibroin grafting with flavonoids: add mushroom tyrosinase to the epigallocatechin solution, and modify the silk fibroin membrane under the condition of continuous aeration. Among them, the silk fibroin membrane The dosage is 100g / L, the dosage of tyrosinase relative to the silk fibroin film is 100U / g silk fibroin film, epigallocatechin 2g / L, t...

Embodiment 2

[0032] (1) Preparation of silk fibroin solution: add the degummed silkworm silk to calcium chloride-ethanol-water (molar ratio 1:1:7) solution, and dissolve it at 70°C to prepare a 25g / L silk fibroin solution. Put the silk fibroin solution into a dialysis bag, dialyze in deionized water for 24 hours, change the water every hour during this period, and filter to obtain silk fibroin aqueous solution after dialysis.

[0033] (2) Preparation of silk fibroin film: Pour a certain amount of silk fibroin solution into a polytetrafluoroethylene (PTFE) mold, spread it flat by spreading it, and dry it at -50°C.

[0034] (3) Enzymatic silk fibroin grafting flavonoids: add tyrosinase to the catechin solution, and modify the silk fibroin film under the condition of continuous oxygenation, wherein the amount of silk fibroin film is 100g / L, the amount of tyrosinase relative to the silk film is 150U / g silk fibroin, catechin 2g / L, the temperature is 30°C, the pH range is 7.0, and the treatment...

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PUM

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Abstract

The invention discloses a preparation method of an enzymatic grafting based antioxidant silk fibroin membrane, and belongs to the biological technical field of textiles. With the help of tyrosinase, antioxidant flavonoids compounds are grafted onto silk fibroin surface after catalytic oxidation; and by crosslinking of tyrosine and the flavonoids compounds in the enzymatic silk fibroin, the antioxidant properties of the silk fibroin membrane is enhanced. The specific processes include silk fibroin solution preparation, silk fibroin membrane preparation, grafting of the flavonoids compounds on the enzymatic silk fibroin and after treatment of the modified silk fibroin membrane. The silk fibroin membrane prepared by the preparation method has improved antioxidant and antimicrobial properties, and the membrane material mechanical properties also are improved. Compared with a silk fibroin membrane modification method based on chemical crosslinking, the enzymatic modification method is low in energy consumption, high in efficiency, less in pollution and in favour of environmental protection.

Description

technical field [0001] The present invention relates to a method for preparing an anti-oxidation silk film based on enzymatic grafting, in particular to a method for preparing an anti-oxidation silk film by using tyrosinase to catalyze the grafting of flavonoids with anti-oxidation activity on the surface of silk fibroin The method belongs to the field of textile biotechnology. Background technique [0002] Silk fibroin derived from mulberry silk has good biocompatibility, and the silk fibroin film processed from it is widely used in the construction of medical tissue engineering materials. In order to improve the application performance of silk fibroin membrane materials, it is necessary to modify and functionalize silk fibroin materials by means of chemical or biological methods. [0003] The commonly used modification methods of silk fibroin film include high temperature and heat treatment, chemical cross-linking modification, modification with other polymer blending met...

Claims

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Application Information

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IPC IPC(8): C08J7/12C08J5/18C08L89/00
Inventor 王平崔莉王树根王强范雪荣袁久刚丁雅静
Owner 广东资恩解码基因检测科技有限公司
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