Fermentation culture medium for 2-keto-L-gulonic acid and fermentation production method of 2-keto-L-gulonic acid

A fermentation medium and production method technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, can solve problems such as affecting yield, and achieve the effects of improving synthesis efficiency, promoting growth and metabolism, and shortening fermentation cycle

Active Publication Date: 2019-04-09
HEILONGJIANG NHU BIOTECH CO LTD +2
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Regarding the improvement of enzyme activity, Patent Document 1 found that Fe 3+ , Mg 2+ and Ca 2+ Can effectively improve the activity of L-sorbose dehydrogenase and L-sorbone dehydrogenase, but Cu 2+ Then inhibit the activity of L-sorbone dehydrogenase, excessive Cu 2+ Lead to autolysis of Bacillus megaterium (B.megaterium) in the fermentation process, thereby affecting the output of 2-KGA, and adding Mg 2+ and Mn 2+ can effectively suppress this phenomenon

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fermentation culture medium for 2-keto-L-gulonic acid and fermentation production method of 2-keto-L-gulonic acid
  • Fermentation culture medium for 2-keto-L-gulonic acid and fermentation production method of 2-keto-L-gulonic acid
  • Fermentation culture medium for 2-keto-L-gulonic acid and fermentation production method of 2-keto-L-gulonic acid

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0048] (2) Preparation of culture medium

[0049] The following medium components all use materials commonly used in the art, and the preparation method is also a preparation method known in the art. The composition of each medium is as follows:

[0050] 1). Solid medium (g / L): L-sorbose 60~80, corn steep liquor 4.0~6.0, urea 11.0~13.0, potassium dihydrogen phosphate 0.5~1.5, anhydrous magnesium sulfate 0.1~0.2, calcium carbonate 4.0 ~6.0, mixed nutrients 0.007~0.009, agar 15~20, pH 6.0~8.0.

[0051] 2). Liquid medium (g / L): L-sorbose 80~120, corn steep liquor 4.0~6.0, urea 11.0~13.0, potassium dihydrogen phosphate 0.5~1.5, anhydrous magnesium sulfate 0.1~0.2, calcium carbonate 4.0 ~6.0, mixed nutrition 0.007~0.009, pH6.0~8.0.

[0052] 3). Fermentation medium (g / L): L-sorbose 80~120, corn steep liquor 4.0~6.0, urea 11.0~13.0, potassium dihydrogen phosphate 0.5~1.5, anhydrous magnesium sulfate 0.1~0.2, calcium carbonate 4.0 ~6.0, mixed nutrition 0.007~0.009, pH6.0~8.0.

[0...

Embodiment 1

[0081] 1) Prepare a mixed nutrient, which consists of 33% cytochrome C and 67% hemoglobin in weight percent. After the preparation is completed, filter and sterilize in an ultra-clean workbench for later use.

[0082] 2) Preparation of fermentation medium (g / L): L-sorbose 120.0, corn steep liquor 5.0, urea 12.0, potassium dihydrogen phosphate 1.0, anhydrous magnesium sulfate 0.1, calcium carbonate 5.0, mixed nutrient 0.008, adjust pH 7.0 , sterilized at 115°C for 25 minutes, in which sorbose was sterilized at 115°C for 25 minutes alone.

[0083] 3) Inoculate the seed solution of the expanded culture in the fermentation medium with a 20% inoculation amount, and use a 15L fermenter for fermentation (70% liquid volume), temperature 32°C, pH 6.8, stirring speed 600rpm, ventilation rate 15L / min, tank pressure 0.03MPa under the conditions of fermentation, synthesis of 2-KGA. In the middle and late stages of fermentation, when the concentration of the mixed nutrient drops to 0.002...

Embodiment 2～8 and comparative example 1～3

[0085] According to the method of Example 1, parameters such as the content of cytochrome C and hemoglobin in the mixed nutrient, the initial concentration of the mixed nutrient, and the feeding concentration of the mixed nutrient were adjusted. Other conditions were the same as in Example 1, and the results were as follows 1 shows:

[0086] Table 1

[0087]

[0088] As can be seen from the data in Table 1, no cytochrome and / or heme was added to the culture medium of Comparative Examples 1-3, compared with it, the fermentation cycle of Examples 1-8 with cytochrome and / or heme added was short And the synthesis efficiency of 2-KGA is high.

[0089] Among them, in the medium of Examples 1-5, not only cytochrome C with a content in the range of 25-67% and heme with a content in the range of 33-75% were added, but also the mixed nutrients in the fermentation medium before inoculation The concentration of the cytochrome is kept in the range of 0.007~0.009g / L, and in the middle ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to view more

Abstract

The invention provides a fermentation culture medium for 2-keto-L-gulonic acid. The fermentation culture medium contains 0.007-0.009 g/L of a mixed nutrient, and the mixed nutrient comprises 25%-67% of cytochrome C and 33%-75% of heme in percentage by weight. The invention also provides a fermentation production method of the 2-keto-L-gulonic acid. The mixed nutrient is supplemented and added whenthe concentration of the mixed nutrient in the fermentation culture medium is decreased to 0.001-0.003 g/L to keep the concentration of the mixed nutrient at 0.001-0.003 g/L until fermentation ends,or pyrroloquinoline quinone is supplemented and added when the online dissolved oxygen value is 20%-40% to keep the concentration at 0.003-0.005 g/L in the culture medium, so that the fermentation period can be shortened, and the synthesis efficiency of the2-keto-L-gulonic acid can be improved.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation. More specifically, the invention relates to a novel 2-keto-L-gulonic acid fermentation medium and a method for producing 2-keto-L-gulonic acid by fermentation. The culture medium and fermentation production method can improve the synthesis efficiency of 2-keto-L-gulonic acid and shorten the fermentation period. Background technique [0002] Vitamin C (VC for short), namely L-Ascorbic acid (L-Ascorbic acid), is a water-soluble vitamin that the human body must ingest from the outside world. It is widely used in medicine, food, cosmetics, feed and other fields. 2-keto-L-gulonic acid (2-KGA for short) is an important precursor for the synthesis of vitamin C. At present, the "two-step fermentation method" is still the main process for the industrial production of VC. The second step of mixed-bacteria fermentation is the key technology of this process. In this technology, only with the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P7/60C12N1/20C12N1/38C12R1/11C12R1/085C12R1/125C12R1/07C12R1/01
CPCC12N1/20C12N1/38C12P7/60C12P39/00
Inventor 司振军李乔平胡柏剡朱永强李宏轩俞柏金陈召峰于凯吕国锋黄国东
Owner HEILONGJIANG NHU BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap