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Antibacterialand and antifungal composition composition comprising mixture of herb essential oil as active ingredient

An effective ingredient and composition technology, applied in the field of antibacterial and antifungal compositions, can solve the problems of little research results of antibacterial and antifungal agents, inconsistent content, insufficient antibacterial and antifungal effects, etc., and achieve excellent antibacterial and antifungal effects. and antifungal activity, the effect of broad antibacterial spectrum

Inactive Publication Date: 2021-01-15
SD BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Despite these research results, existing plant extracts are disadvantageous in that they are difficult to formulate due to inconsistencies in the content of the components contained depending on the extraction site, time, and method
In addition, when only a single component of the plant extract is isolated and used to overcome the above disadvantages, it does not have sufficient antibacterial and antifungal effects, and cannot be used in the industrial field
[0008] But so far, there has not been sufficient research on the antibacterial and antifungal synergistic activities of a single component isolated from a plant extract. little success

Method used

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  • Antibacterialand and antifungal composition composition comprising mixture of herb essential oil as active ingredient
  • Antibacterialand and antifungal composition composition comprising mixture of herb essential oil as active ingredient
  • Antibacterialand and antifungal composition composition comprising mixture of herb essential oil as active ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1. The preparation of herbal essential oil

[0063] For 17 kinds of plant materials in the following [Table 1], each 0.5 kg of plant materials was washed with purified water, dried, pulverized and pulverized to obtain a powder. After each powdery plant material was heated to 100° C., water vapor was collected and cooled to obtain essential oil.

[0064] [Table 1]

[0065]

[0066]

Embodiment 2

[0067] Embodiment 2. the antibacterial test of herbal essential oil

[0068] 2-1. Using the paper diffusion method to test the antibacterial ability of a single herbal essential oil

[0069] The antibacterial activity of the 17 kinds of herbal essential oils prepared in Example 1 was verified by the commonly used disc diffusion method.

[0070] Escherichia coli KCTC2571, Pseudomonas aeruginosa KCTC2513, Staphylococcus aureus KCTC3881, Candida albicans KCTC7965 and Aspergillus niger KCTC6317 (KCTC , Bioresource Center, Korea Institute of Biotechnology).

[0071] Use LB medium to cultivate Escherichia coli (E.coli), use NA medium to cultivate Pseudomonas aeruginosa (P.aeruginosa) and Staphylococcus aureus (S.aureus) strains, and use YM medium and PDA medium to cultivate white Candida (C.albicans) and Aspergillus niger (Aspergillus niger) strains. By obtaining 2 to 3 colonies (1×10 7 CFU / mL) to inoculate each bacteria. The herbal essential oil prepared in Example 1 was dis...

Embodiment 3

[0084] Embodiment 3. the antibacterial test of the optimal proportioning of selected mixed herbal essential oil

[0085] 3-1. Verification of the synergistic effect of lemongrass and marjoram essential oils

[0086] In order to confirm the interaction between the lemongrass of Test Example 1 and the marjoram essential oil of Test Example 2, the minimum inhibitory concentration (MIC) of the mixed essential oils was evaluated.

[0087] Since there is no standardized test method for confirming antibacterial synergy, a checkerboard assay (checkerboard assay) for confirming antibacterial synergy by combining antibacterial substances was used. The test for measuring the antibacterial ability was set as 6 rows×6 columns, and the mixed essential oils of lemongrass and marjoram to be measured were serially diluted 2 times at the MIC concentration on a 48-well plate, and the lemongrass was divided along the horizontal axis, Marjoram was serially diluted in wells along the vertical ax...

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Abstract

The present invention relates to an antibacterial and antifungal composition comprising, as an active ingredient, a herbal mixed essence of marjoram, lemon grass, dalbergia odorifera, citronella, melaleuca alternifolia, and thyme. The herbal mixed essence of marjoram, lemon grass, dalbergia odorifera, citronella, melaleuca alternifolia and thyme shows excellent antibacterial and antifungal activity by directly influencing harmful microorganisms, and can be effectively applied to pharmaceutical compositions, cosmetic compositions, preservatives for preserving foods or feeds and the like.

Description

technical field [0001] The present invention relates to an antibacterial and antifungal composition containing herbal mixed essential oils as active ingredients, more specifically, to a herbal composition containing marjoram, lemongrass, black Dalbergia, citronella, melaleuca alternifolia and thyme Antibacterial and antifungal composition mixed with essential oils as active ingredients. Background technique [0002] Some of the substances that play an important role in the process of maintaining homeostasis are biologically active substances derived from various organisms. So far, many researches on biologically active substances have been carried out, and among them, the research on antibacterial substances isolated from microorganisms, plants or animals is a very important field in the field of life science and medicine. [0003] All living things produce antibacterial and antifungal substances in order to survive, and many studies have been conducted on microorganisms th...

Claims

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Application Information

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IPC IPC(8): A61K36/899A61P31/04A61P31/10A23K30/00A23L3/3472A61K8/9794A61K8/9789A61Q17/00A61Q19/00
CPCA23L3/3472A23V2002/00A61K36/48A61K36/53A61K36/61A61K36/899A61K2800/524A61Q17/005A61Q19/00A23K30/00A61K8/9789A61K8/9794A61P31/04A61P31/10A61K2300/00A23V2200/10A23V2250/21Y02A50/30A61K36/88
Inventor 朴卨雄杨贞恩黃恩宣李根夏元京和申志硏
Owner SD BIOTECH