A nucleic acid detection cartridge and detection method based on magnetic bead transfer and valve-controlled pipetting

Abstract

The invention discloses a nucleic acid detection cartridge and detection method based on magnetic bead transfer and valve-controlled pipetting. The cartridge body is divided into a nucleic acid extraction area and an amplification detection area. The amplification detection area is equipped with a rotary valve tube, a PCR Tube, gas pump interface, gas channel, liquid channel, the gas channel extends from the rotary valve tube to the mixing tube and the gas pump interface, the liquid channel extends from the rotary valve tube to the elution tube, the mixing tube and the PCR tube respectively, and the rotary valve tube is set There are rotary valves for controlling the opening and closing of the gas and liquid passages. The invention can be directly added to clinically collected throat swabs without any treatment, and the nucleic acid extraction can be realized by driving the magnetic rod and the magnetic rod cover externally, and the switch of the relevant liquid passage and gas passage can be realized by controlling the rotary valve, and can be controlled under the action of the external pump. Realize the directional transfer of the liquid, and finally perform real-time fluorescent PCR amplification detection in the PCR tube, and realize "sample in, result out" simply and efficiently.

Description

technical field [0001] The invention relates to a detection device and a detection method, in particular to a nucleic acid detection cartridge and a detection method based on magnetic bead transfer and valve-controlled pipetting. Background technique [0002] Thanks to the advantages of high sensitivity and high specificity of nucleic acid detection, it is more and more widely used in various fields of biomedicine, especially in the field of infectious disease detection. In the prevention and control of infectious diseases, timely and accurate diagnosis of suspected cases can win valuable time for the prevention and control of the epidemic. However, nucleic acid detection requires cumbersome nucleic acid extraction and time-consuming PCR amplification detection steps, which require professional experimenters to complete on different experimental equipment. The whole process takes several hours, which not only greatly reduces the efficiency of nucleic acid detection, but also...

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Problems solved by technology

However, nucleic acid detection requires cumbersome nucleic acid extraction and time-consuming PCR amplification detection steps, which require professional experimenters to complete on different experimental equipment. The whole process takes several hours, which not only greatly reduces the efficiency of nucleic acid detection, but also due to The transfer of samples between different devices during the process may also cause cross-contamination, which will affect the test results

[0003] Based on the above problems, some cartridge-type integrated nucleic acid detection systems have been launched on the market, which can integrate nucleic acid extraction and amplification detection in a cartridge or microfluidic chip. However, most of the cartridges currently use driver The working principle of the liquid, the cartridge structure is complicated, the cost is expensive, and the work efficiency is not high. For some special samples such as throat swab samples, it requires cumbersome steps such as sample inactivation and pathogen release in the early stage, and it is impossible to directly add samples for detection. The field of infectious disease detection has certain limitations

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