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Method for optimizing sweet potato polysaccharide extraction conditions based on response surface method

A technology of sweet potato polysaccharide and response surface method, which is applied in the field of optimization of sweet potato polysaccharide extraction conditions based on response surface method, can solve the problems of waste of raw materials and low extraction rate of sweet potato polysaccharide, reduce raw material loss, ensure scientific extraction, and simple extraction process Effect

Pending Publication Date: 2021-01-19
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Application Information

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Problems solved by technology

[0005] In the prior art, the extraction rate of sweet potato polysaccharides is low, which easily causes waste of raw materials

Method used

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  • Method for optimizing sweet potato polysaccharide extraction conditions based on response surface method
  • Method for optimizing sweet potato polysaccharide extraction conditions based on response surface method
  • Method for optimizing sweet potato polysaccharide extraction conditions based on response surface method

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Embodiment Construction

[0040] Below in conjunction with embodiment, the present invention is further described, and following embodiment is descriptive, not limiting, can not limit protection scope of the present invention with following embodiment.

[0041] The raw materials used in the present invention, if no special instructions, are conventional commercially available products, the methods used in the present invention, if no special instructions, are conventional methods in this area, and the quality of each material used in the present invention is routine use quality.

[0042] A method for optimizing sweet potato polysaccharide extraction conditions based on response surface methodology, the steps are as follows:

[0043](1) Peel the sweet potato and cut it into strips, put it into a freeze dryer at -70°C to freeze dry, take it out overnight and put it in an oven at 60°C to continue drying for more than 8 hours, and finally crush it into sweet potato powder with a grinder, pass through 40 me...

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Abstract

The invention relates to a method for optimizing sweet potato polysaccharide extraction conditions based on a response surface method. The method comprises the following steps: peeling and cutting sweet potatoes into strips, performing freezing-drying, drying, smashing and sieving; extracting sweet potato polysaccharide by a water extraction and alcohol precipitation method, and determining variable parameters of sweet potato polysaccharide extraction by a response surface method; adding petroleum ether for degreasing, and adding ethanol into the precipitate; adding water and performing extracting; performing centrifuging, performing concentrating, adding absolute ethyl alcohol, standing overnight, and performing centrifuging to prepare a crude polysaccharide solution; removing protein, performing decolorizing, performing concentrating and performing dialyzing to obtain crude sweet potato polysaccharide. Variable parameters of sweet potato polysaccharide extraction are determined by adopting a response surface method, and a four-factor three-level response surface analysis test is designed, so the optimal extraction process condition of sweet potato polysaccharide is optimized, anda reference basis is provided for comprehensive development and utilization of sweet potatoes. The actual value is very close to the theoretical predicted value. Therefore, the response surface optimization of the Design-Expert software on the sweet potato polysaccharide extraction process condition is reliable, and the method has a practical value.

Description

technical field [0001] The invention belongs to the field of food technology, in particular to a method for optimizing sweet potato polysaccharide extraction conditions based on response surface methodology. Background technique [0002] Sweet potato (scientific name: Dioscorea esculenta (Lour.) Burkill) herb plant, belongs to Convolvulaceae sweet potato species in classification, has another name called sweet potato, sweet potato, sweet potato, sweet potato, sweet potato and yam. Sweet potato is not only nutritious, but also contains various bioactive substances such as protein, polysaccharide, polyphenol, dietary fiber, vitamins and minerals. Sweet potato active polysaccharides also have the functions of anti-oxidation, anti-tumor, anti-mutation, enhancing immunity and lowering blood fat. [0003] The principle of water extraction and alcohol precipitation is to use ethanol to precipitate polysaccharides by utilizing the insoluble properties of polysaccharides in ethanol;...

Claims

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Application Information

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IPC IPC(8): C08B37/00G01N21/31G16B5/00
CPCC08B37/0003G01N21/31G16B5/00
Inventor 王春玲杜船张倩周韬刘爽
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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