Flammulina velutipes suitable for industrial cultivation and molecular identification method of flammulina velutipes

A technology for classification and naming of Flammulina velutipes, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, and measurement/inspection of microorganisms, can solve problems such as irregular fruiting, yellowish color, and low yield per unit area of ​​Flammulina velutipes species, and achieve mushroom production. The effect of white body and small mushroom cap

Active Publication Date: 2021-01-26
FUJIAN AGRI & FORESTRY UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention will solve the technical problems of low yield per unit area, irregular fruiting, and yellowish color of the species of Flammulina velutipes used in the existing production,

Method used

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  • Flammulina velutipes suitable for industrial cultivation and molecular identification method of flammulina velutipes
  • Flammulina velutipes suitable for industrial cultivation and molecular identification method of flammulina velutipes
  • Flammulina velutipes suitable for industrial cultivation and molecular identification method of flammulina velutipes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: select the Flammulina velutipes 'F0321' and 'F0323' that are mainly planted as hybrid parents, select this test to prepare 180 combinations in total, and have 59 hybrids that contain lock joints through microscopic examination, and screen out 50 of them. After the fruiting test of the better hybrid strains, 11 hybrids capable of fruiting were selected for secondary screening. Among the 11 re-screened hybrid strains, 4 hybrid strains have an average yield of more than 400g, among which Flv0088 has better comprehensive properties and has reached the expected breeding goal. This hybrid strain is named 'Nongwanjin No. 9' and will be used for subsequent batches test.

[0017] Choose Flammulina velutipes 'F0321' and 'F0323' as the hybrid parents, and select 20 single-spore strains of 'F0321' and 20 single-spore strains of 'F0323', which grow faster and have more developed aerial hyphae , paired according to the full combination method, a total of 60 combination...

Embodiment 2

[0024]Example 2: Using SV detection technology to compare the DNA differences between this strain and its parent.

[0025] Test materials: 5 strains of Flammulina velutipes, among which the strain to be tested is 'Nongwanjin No. 9', control variety 1: 'Nongwanjin No. 8' (Flv0012), control variety 2: 'Nongjin No. 3' (F0303), F0321, F0323.

[0026] Inspection method: According to the genome sequencing data, Kmer subtractive assembly technology is used to obtain differential fragments (SV), and several SV sequences are selected accordingly to design specific primers to amplify the samples to be tested. (For references see Alkan, C., Coe, B. P. & Eichler, E. E. Genome structural variation discovery and genotyping. Nature reviews. Genetics, 2011(12), 363–376; Zhu Fengli et al., Whole Genome Resequencing Analysis of Korean Chizhi Zhizhi, World Science and Technology-Modernization of Traditional Chinese Medicine, 21(4)764-774.). SV test primer sequences were synthesized by Qingke...

Embodiment 3

[0056] Example 3: 'Nongwanjin No. 9' has carried out many stable yield tests. The results show that 'Nongwanjin No. 9' meets the requirements of industrial cultivation, and its yield is significantly higher than that of the control variety 'Nongwanjin No. 8' Henongjin No. 3 (F0303).

[0057] Multi-batch stable yield and productive test strains: 'Nongwanjin No. 8', 'Nongjin No. 3' (Flv0303).

[0058] Test location: Fujian Wanchen Biotechnology Co., Ltd.

[0059] Test arrangement: uniform production of strains, factory cultivation at the test site according to local production management methods, bottle cultivation, and multi-zone mobile bed frame cultivation (plastic bottle, 0.43kg (dry material) / bottle). See Table 2 for specific cultivation information:

[0060] Table 2 Multi-batch test information table

[0061]

[0062] Mycelial growth test results: During the experimental seed production period, the mycelium of 'Nongwanjin No. 9' was thicker in PDA, and the optimum gr...

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Abstract

The invention discloses a flammulina velutipes strain 'Nongwanjin No. 9', belongs to flammulina velutipes. The flammulina velutipes strain is preserved in China General Microbiological Culture Collection Center (CGMCC). The 'Nongwanjin No.9' strain provided by the invention has the quality characteristics of pure white mushroom body, small mushroom cap, inward curling, orderliness, uniformity, compact mushroom root, uniform longitudinal section pores, hard stipe and the like of the excellent flammulina velutipes strain. The conditions that the parent F0321 pileus is relatively thin and the stipe is relatively soft are improved; the defects that cultivation and management difficulty of another parent F0323 is high, and the defects of fungus covering, terminal bud and the like are easy to occur are overcome; the average yield per unit area of the strain is 12.9% higher than that of a control strain 'Nongwanjin No.8' (significant difference), and is 56.2% higher than that of a control strain 'Nongwanjin No.3' (significant difference). The 'Nongwanjin 9' commodity is good in appearance, suitable for market requirements, excellent in comprehensive performance and suitable for industrialproduction, and has a good popularization prospect.

Description

technical field [0001] The invention relates to a strain of Flammulina velutipes, in particular to a Flammulina velutipes Nongwanjin No. 9 suitable for industrialized cultivation and a molecular identification method thereof. Background technique [0002] Flammulina velutipes [ Flammulina velutiper (Fr.) Sing] belongs to Basidiomycotina, Laminomycetes, Agaricaceae, Agaricaceae, and Flammulina genus. It is famous for its smooth and tender cap, crisp handle, rich nutrition, and delicious taste. Fujian Province is the main planting area of ​​Flammulina velutipes in South China. With the expansion of the cultivation scale of Flammulina velutipes, the market has produced higher and higher standards for judging the cultivation, color and yield of Flammulina velutipes, such as the thickness and uniformity of the stipe, the gap of the cut surface, the brittleness of the stipe, and freshness. Persistence, cap size, mushroom body color and other evaluation criteria. At present, un...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12Q1/6895C12R1/645
CPCC12N1/14C12Q1/6895C12Q2600/156C12R2001/645C12N1/145
Inventor 刘芳江玉姬李博周志兵江益民岑光红蔡鹏赖丽红王旭华谢宝贵
Owner FUJIAN AGRI & FORESTRY UNIV
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