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Heavy metal ion indicator based on sip-me/dmps reversible reaction and its application

A technology of heavy metal ions and indicators, which can be used in material analysis by observing the influence of chemical indicators, analysis by chemical reaction of materials, fluorescence/phosphorescence, etc., which can solve the problems of complex probe synthesis and single detection. , to achieve the effect of good photostability, rapid response and simple design

Active Publication Date: 2022-01-28
ZHENGZHOU UNIV
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Problems solved by technology

[0004] The invention proposes an indicator and the application of the reversible reaction between silpiro red (Sip-Me) and sodium 2,3-dimercaptopropanesulfonate (DMPS) in the detection of heavy metal ions, which solves the problem of the current detection of heavy metal probes. Synthesize Complex, Detect Single Issues

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  • Heavy metal ion indicator based on sip-me/dmps reversible reaction and its application
  • Heavy metal ion indicator based on sip-me/dmps reversible reaction and its application
  • Heavy metal ion indicator based on sip-me/dmps reversible reaction and its application

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Embodiment Construction

[0053] The technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0054] 1. Fluorescence intensity changes of the reaction between silpiro red and sodium 2,3-dimercaptopropanesulfonate.

[0055] Prepare a PBS (10 mM) buffer solution with a pH of 7.4; weigh silpiro red and dissolve it in acetonitrile to accurately prepare a 2 mM probe stock solution; prepare 4 mM sodium 2,3-dimercaptopropanesulfonate. After adding 2 mL of PBS buffer solution to the cuvette, add 5 µL of silpiro red stock solution with a concentration of 2 mM, and then add 5 µL of sodium 2,3-dime...

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Abstract

The invention provides a heavy metal ion indicator based on Sip-Me / DMPS reversible reaction and its application. Silpiro red and sodium 2,3-dimercaptopropanesulfonate are mixed and reacted at room temperature according to the volume ratio of 1:2, and the reaction Afterwards, the heavy metal ions were detected by using silpirole red as the fluorophore and the dithiol group as the recognition site. At the same time, the water-absorbing bomb absorbed with silpiro red and 2,3-dimercaptopropanesulfonate solution was used as an indicator, and the reaction between silpiro red and 2,3-dimercaptopropanesulfonate made the fluorescence quenched, and then mixed with Heavy metal ions react to form complexes to remove dimercaptosuccinic acid, so that the fluorescence of the system is restored, and the presence of mercury ions, zinc ions, nickel ions, cadmium ions, copper ions and cobalt ions, six heavy metal ions can be detected.

Description

technical field [0001] The invention relates to the field of heavy metal ion reaction strategy design, in particular to a heavy metal ion indicator based on a Sip-Me / DMPS reversible reaction and its application. Background technique [0002] Heavy metal ions are not only highly concerned by scientific chemists, biologists, and environmental scientists, but also increasingly by the general public. Although certain heavy metal ions play important roles in living systems, they are highly toxic and can cause serious environmental and health problems. Mercury, for example, is one of the most toxic heavy metals. It is a non-essential harmful element for living things. Normally, mercury is liquid and may evaporate at room temperature. If the mercury content in the soil is too high, it will enrich the mercury in the plants. Plants produce toxic effects, and if they enter the human body through the food chain, they can have harmful effects on human function, including increased r...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N21/64
CPCG01N21/78G01N21/6402G01N21/643G01N21/6428G01N2021/6432
Inventor 杨冉孙远强郭逸菲李朝辉屈凌波
Owner ZHENGZHOU UNIV
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