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Process for producing fluorescent reagent

A fluorescent reagent and solvent technology, applied in the field of preparation of fluorescent reagents, can solve the problems of high fluorescence quantum efficiency detection of components, unstable derivative products, low reactivity, etc., and achieve good application prospects, stable derivative products, and derivatization speed. quick effect

Inactive Publication Date: 2008-05-07
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

According to previous reports, the existing reagents have the following defects: low reactivity, slow derivatization speed, unstable derivative products, large fluorescence quantum efficiency of components before and after elution detection

Method used

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  • Process for producing fluorescent reagent
  • Process for producing fluorescent reagent
  • Process for producing fluorescent reagent

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Embodiment Construction

[0014] Add 46g of aniline o-benzoate and 100mL of concentrated sulfuric acid into a 500mL flask, heat in a boiling water bath for 4 hours, then pour it into a 1-liter beaker, pay attention to let the reaction solution flow down the wall of the vessel to reduce splashing of the solution. After boiling for 5 minutes, the yellow precipitate was filtered out, and the filtered solid was fully washed with water, and the crude acridone was air-dried.

[0015] Add 30g of the above-mentioned acridone and 150mL of dimethyl sulfoxide into a 500ml round-bottomed flask, start electromagnetic stirring and heat, add 10g of potassium hydroxide in batches within 20 minutes, keep the solution temperature at 100°C and heat under reflux and stir for 30 minutes , 40g of 2-bromoethanol was added within 1 hour, and the temperature continued to be kept at 100° C., and the reaction was continued under reflux and stirring for 2.5 hours, and the solution gradually turned dark brown. Cool the mixture in ...

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Abstract

The present invention relates to a preparation method of fluorescent reagent. Said method includes the following steps: using ortho-benzoxyaniline as mother body for synthesis, under the action of concentrated sulfuric acid reflux heating for 2-4hr in boiling water bath to form acridone; making acridone be reacted with 2-bromoethanol in dimethyl sulphoxide solvent to obtain 2-(N-acridone group)-ethyl alcohol; stirring 2-(C-acridone group)-ethyl alcohol and tricyanogen chloride in methylene dichloride solution at 0-5 deg.C and making them be reacted for 16-24 hr so as to obtain the target product. Said invention also provides the characteristics of said fluorescent reagent and its application range.

Description

technical field [0001] The invention relates to a preparation method of a fluorescent reagent, in particular to a preparation method of 2-(N-acridone)-ethoxy-4,6-dichlorotriazine. Background technique [0002] The research on the fluorescence properties of acridone and its derivatives mainly focuses on the detection of several common simple structure derivatives as derivatization reagents for biomolecules, see attached table 1. Due to the special structural properties of acridone, in the existing reports, the research mainly focuses on the discussion of its quantum properties. Such as the transition of π-electrons, the influence of solvent properties on the fluorescence properties of acridone derivatives, etc. According to previous reports, the existing reagents have the following defects: low reactivity, slow derivatization speed, unstable derivative products, and large fluorescence quantum efficiencies of components before and after elution. Compared with existing fluore...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/00G01N21/33C09K11/61C09K11/00
Inventor 张玉奎张琳张丽华张维冰
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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