Application of pyrithione zinc in treatment of lung cancer

A technology for zinc pyrithione and lung cancer, applied in the application field of zinc pyrithione in the treatment of lung cancer, can solve the problem that the broad-spectrum treatment effect of zinc pyrithione on lung cancer has not been reported, and achieve the inhibition of lung cancer metastasis, low price, and broad-spectrum treatment active effect

Pending Publication Date: 2021-02-09
XIAMEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] So far, the broad-spectrum therapeutic effect of zinc pyrithione in lung cancer has not been reported

Method used

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  • Application of pyrithione zinc in treatment of lung cancer
  • Application of pyrithione zinc in treatment of lung cancer
  • Application of pyrithione zinc in treatment of lung cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1 Experiment of the Killing Effect of Zinc Pyrithione on Different Tumor Cells of Lung Cancer

[0056] The experimental method is to measure the viability of CCK-8 cells. CCK-8 reagent contains WST-8 [chemical name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfone Acid phenyl)-2H-tetrazole monosodium salt], which is reduced by dehydrogenase in cells under the action of electron carrier 1-methoxy-5-methylphenazinium dimethyl sulfate (1-MethoxyPMS) It is a highly water-soluble yellow formazan product (Formazan dye). The amount of formazan produced is directly proportional to the number of living cells. This feature can therefore be used directly for cell proliferation and toxicity assays.

[0057] The specific experimental steps are as follows: cells are digested with trypsin, dispersed into single cells, and suspended in corresponding DMEM or RIPM1640 medium. Cells were seeded in 96-well culture plates, 5000cells / well, placed in a 37°C / carbon di...

Embodiment 2

[0067] Example 2 Zinc pyrithione induces apoptosis of lung cancer cells

[0068] The experimental method is to use Annexin V-FITC / PI double staining method, and use flow cytometry to detect cell apoptosis.

[0069] The principle of the Annexin V-FITC / PI double staining method for detecting cell apoptosis is as follows: the distribution of phospholipids in the normal cell membrane is asymmetrical, and the inner surface of the membrane contains negatively charged phospholipids (such as phosphatidylserine, PS), while the outer surface of the membrane contains absolute most neutral phospholipids. In the early stage of apoptosis, the PS in the cell membrane will flip from the inside to the surface of the cell membrane. Annexin V is a calcium-dependent phospholipid-binding protein that can specifically bind to PS with high affinity. Propidium iodide (PI) is a nucleic acid dye that cannot penetrate intact cell membranes, while the cell membranes of early apoptotic cells are intact ...

Embodiment 3

[0079] Example 3 Zinc pyrithione inhibits tumor cell migration experiment

[0080] The experimental method is cell scratch experiment. The cell scratch test is the simplest and economical in vitro test method to study cell migration. The method works by artificially creating a blank area, called a "scratch," on the confluent monolayer of cells when the cells grow to the point of confluence into a monolayer. The cells at the edge of the scratch will gradually enter the blank area to heal the "scratch". The stronger the cell migration ability, the shorter the time to heal the scratch.

[0081] The specific experimental steps are as follows: cells are digested by trypsin for 3 minutes, and medium is added to terminate the digestion reaction. Then gently pipette to mix the cells, and evenly seed the cells in the six-well plate. Place cells in 5% CO 2 Incubate for 8-24 hours in a 37°C incubator. The number of cells varies with different cells, and it can be confluent overnight...

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Abstract

The invention relates to a purpose of pyrithione zinc, a stereisomer thereof or various pharmaceutically acceptable modification objects of the pyrithione in preparation of medicines used for treatinglung cancer. The pyrithione zinc can adopt various mechanisms of inducing tumor cells to generate apoptosis, inhibiting a tumor cell cycle, inhibiting tumor migration and the like to inhibit lung cancer cell proliferation so as to achieve an effect of inhibiting tumor growth or clearing tumors.

Description

technical field [0001] The invention relates to the field of medicinal chemistry, in particular to the application of zinc pyrithione in the treatment of lung cancer. Background technique [0002] Cancer seriously endangers the health of human beings around the world. According to the "2018 Global Cancer Statistics" report published by the official journal of the American Cancer Society "Journal of Cancer for Clinicians", it is estimated that there will be about 18.1 million new cancer cases and 960 late cancer deaths in the world in 2018. Among them, lung cancer is the largest cancer in the world, and its incidence rate (11.6%) and mortality rate (18.4%) are both in the first place. Lung cancer has a high degree of complexity and heterogeneity due to its complex causes and pathogenesis. Lung cancer is divided into two main types clinically, non-small cell lung cancer and small cell lung cancer. Therapeutic drugs against lung cancer have very important prospects, but at th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/06A61K31/555A61P11/00A61P35/00
CPCA61K45/06A61K31/555A61P11/00A61P35/00
Inventor 陈毅歆王国松洪俊平吴倩陈瑞琪黄鹏飞夏宁邵
Owner XIAMEN UNIV
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