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Nano-carbon loaded cluster-state copper nano-enzyme as well as preparation method and application thereof

A nano carbon and copper nano technology, applied in the fields of botanical equipment and methods, applications, chemical instruments and methods, etc., can solve problems such as not yet established a complete theoretical system, achieve excellent oxidase catalytic activity, excellent catalytic antibacterial performance, improve The effect of atom utilization

Pending Publication Date: 2021-02-09
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on nanozymes is still in the development stage, and a complete theoretical system has not yet been established. The research on nanozymes has always attracted much attention.

Method used

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  • Nano-carbon loaded cluster-state copper nano-enzyme as well as preparation method and application thereof
  • Nano-carbon loaded cluster-state copper nano-enzyme as well as preparation method and application thereof
  • Nano-carbon loaded cluster-state copper nano-enzyme as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Nanocarbon-supported clustered copper nanozyme (Cu 3 / ND@G)

[0030] (1) preparation method comprises the steps:

[0031] 1. Take 200mg of nano-diamond / graphene (ND@G) in a round bottom flask, add 30mL of deionized water, disperse it by ultrasonic, adjust the pH value of the system to about 11, and obtain ND@G suspension. Then, under magnetic stirring at 100°C in an oil bath, 4 mL of copper nitrate aqueous solution (containing 0.25 mg·mL -1 Cu) was added dropwise to the ND@G suspension and kept stirring for 1 h. Finally, the resultant was naturally cooled to room temperature and the solid matter was collected, washed, and dried to obtain a nanocomposite material, denoted as Cu-NPs / ND@G.

[0032] 2. Spread the nanocomposite Cu-NPs / ND@G obtained in step 1 in a porcelain boat, put it in a tube furnace, and gradually raise the temperature to 600°C, 2 Under the condition of reduction for 1 hour, the nano-carbon supported clustered copper nanozyme was obtained, denoted as...

Embodiment 2

[0038] Nanocarbon-supported clustered copper nanoenzyme Cu 3 Study on Oxidase Activity of / ND@G

[0039] (1) Cu-NPs / ND@G and Cu 3 / ND@G's TMB experiment

[0040] Determination of Cu-NPs / ND@G and Cu by TMB experiments 3 / ND@G simulates oxidase activity.

[0041] Method: 10 μL concentration is 0.5mg·mL -1 Cu-NPs / ND@G (or Cu 3 / ND@G) and 20 μL of TMB (3,3',5,5'-tetramethylbenzidine) at a concentration of 20 mM were added to a centrifuge tube containing 970 μL of sodium acetate-acetic acid buffer [100 mM (pH=4.0)] middle. Study of Cu-NPs / ND@G and Cu by measuring the UV absorption change of the oxidized form of TMB at a wavelength of 652 nm 3 / ND@G catalytic oxidation of TMB, and then calculate Cu-NPs / ND@G and Cu 3 Oxidase activity of / ND@G.

[0042] Figure 4 For Cu-NPs / ND@G and Cu 3 / ND@G's TMB experiment map. Figure 4 A is Cu-NPs / ND@G and Cu 3 A typical Michaelis-Menten curve for / ND@G, given by Figure 4 As can be seen in middle A, it can be seen that the oxidase...

Embodiment 3

[0044] Nanocarbon-supported clustered copper nanoenzyme Cu 3 Application of / ND@G in antibacterial

[0045] Antibacterial experiment, including the following steps:

[0046] 1) Preparation of LB medium: Weigh 2.5g of sodium chloride, 2.5g of peptone, 1.25g of yeast extract powder, add water to 250mL, adjust its pH to 7.2-7.4 with NaOH solution, and obtain LB liquid medium; Add 2% agar to the culture medium to obtain a solid culture medium, sterilize the culture medium in a high-pressure steam sterilizer at 121°C for 30 minutes, and refrigerate for future use.

[0047] 2) Pick Escherichia coli strains (E.coli ATCC 15597) with an inoculation loop, streak on the solid medium, culture in a 37°C incubator for 24 hours, pick a single colony in the liquid medium, and shake at a constant temperature of 37°C Cultivate in a container for 11 hours to obtain a bacterial suspension with an OD value of 2.162.

[0048] 3) Take 5 mL of bacterial suspension and centrifuge for 20 minutes in ...

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Abstract

The invention discloses a nano-carbon loaded cluster-state copper nano-enzyme as well as a preparation method and application thereof. The nano-carbon loaded copper in the cluster state is used as a nano-enzyme, oxygen in air can be catalyzed to be decomposed at room temperature to generate active oxygen substances, and the active oxygen substances act on bacteria to achieve the sterilization purpose. In the nano-enzyme, the copper exists in the form of atomic-scale dispersed clusters, so that the enzyme catalytic activity of copper is remarkably improved, and the antibacterial rate can reach100%. The synthesis process is simple and convenient, the reaction process is easy to control, and the prepared nanocarbon-loaded cluster-state copper nanoenzyme shows excellent catalytic antibacterial performance and has potential application in the field of biomedicine.

Description

technical field [0001] The invention relates to the technical field of nanozyme-catalyzed antibacterial, in particular to the application of a nano-carbon supported atomic-level dispersed cluster copper nanocomposite material as nanozyme-catalyzed antibacterial. Background technique [0002] Nanozyme is a nanocomposite material with enzymatic catalytic activity, which not only has the unique properties of nanomaterials, but also has the catalytic activity of mimicking natural enzymes. Due to its strong and diverse enzymatic catalytic activities, it has attracted extensive attention for potential applications in biosensors, cancer therapy, and antibacterial fields. At present, the research on nanozymes is still in the development stage, and a complete theoretical system has not yet been established. The research on nanozymes has always attracted much attention. [0003] With the progress and development of modern science and technology, more and more germs appear, which seri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J23/72A01N59/20A01N59/00A01N25/08A01P3/00A01P1/00
CPCB01J23/72A01N59/20A01N59/00A01N25/08Y02A50/30
Inventor 杨黎妮孟凡池曲晓月李涛刘霄刘洪阳夏立新
Owner LIAONING UNIVERSITY
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