Method for inhibiting sulfate reducing bacteria in high-temperature oil field water
A sulfate, water technology, applied in microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of limited, low inhibition rate and high cost
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Embodiment 1
[0035] The acquisition and identification of embodiment 1 Thermobacterium petroleum ZHY-HL24
[0036] 1. Obtaining of Thermobacterium petroleum ZHY-HL24
[0037] A bacterial strain named ZHY-HL24 was isolated from the produced fluid of S oilfield in Bohai Sea. Its separation method is as follows: inoculate the output liquid into the enrichment medium (NaCl 5.00g / L, MgCl 2 ·6H 2 O0.28g / L, CaCl 2 0.55g / L, NH 4 Cl0.24g / L, KCl0.10g / L, KH 2 PO 4 0.20g / L, sodium acetate 2.00g / L, NaNO 3 0.85g / L, NaHCO 3 0.20g / L, Trace elements 0.5mL / L and Vitamin 0.5mL / L, pH 7.0), anaerobic enrichment culture at 55°C. Then take 0.5mL and insert it into an anaerobic tube (with the same medium as above, and add 1.2% plant gel), and perform anaerobic roller tube separation at 55°C, pick a single colony from it, and perform repeated purification to obtain the strain.
[0038] The strain is Petrothermobacter organivorans of the present application, and its registration number in the General Micro...
Embodiment 2
[0046] Embodiment 2 Thermobacterium petroleum ZHY-HL24 inhibits H 2 S
[0047] 1. Preparation of Thermobacterium petroleum ZHY-HL24 fermentation broth
[0048] The pyrobacterium petroleum ZHY-HL24 that embodiment 1 obtains is inoculated in the nitrate reducing bacteria substratum (substrate composition: solute is: NaCl 5.00g / L, MgCl 2 ·6H 2 O0.28g / L, CaCl 2 0.55g / L, NH 4 Cl0.24g / L, KCl0.10g / L, KH 2 PO 4 0.20g / L, sodium acetate 2.00g / L, NaNO 3 0.85g / L, NaHCO 3 0.20g / L; the solvent is water; adjust the pH to be 7.0; sterilize at 121°C for 20 minutes) in anaerobic culture at 55°C for 14 days to obtain the fermented liquid of Thermobacterium petroleum ZHY-HL24, and the petroleum heat in the fermented liquid of Thermobacterium petroleum ZHY-HL24 The content of Bacillus ZHY-HL24 was 1.5×10 8 cfu / mL.
[0049] 2. Preparation of Sulfate Reducing Bacteria Liquid
[0050] Bohai S oilfield production water was inoculated into sulfate-reducing bacteria enrichment medium accordin...
Embodiment 3
[0063] Embodiment 3 Thermobacterium petroleum ZHY-HL24 inhibits H 2 S
[0064] 1. Preparation of Thermobacterium petroleum ZHY-HL24 fermentation broth
[0065] The pyrobacterium petroleum ZHY-HL24 that embodiment 1 obtains is inoculated in the nitrate reducing bacteria substratum (substrate composition: solute is: NaCl 5.00g / L, MgCl 2 ·6H 2 O 0.28g / L, CaCl 2 0.55g / L, NH 4 Cl 0.24g / L, KCl 0.10g / L, KH 2 PO 4 0.20g / L, sodium acetate 2.00g / L, NaNO 3 0.85g / L, NaHCO 3 0.20g / L, the solvent is water; adjust the pH to 7.0; sterilize at 121°C for 20 minutes). Anaerobic culture at 60°C for 14 days, the fermented liquid of Thermobacterium petroleum ZHY-HL24 was obtained, and the content of Thermobacterium petroleum ZHY-HL24 in the fermented liquid of Thermobacterium petroleum ZHY-HL24 was 1.5×10 7 cfu / mL.
[0066] 2. Preparation of Sulfate Reducing Bacteria Liquid
[0067] The Shengli Oilfield produced water was inoculated into the sulfate-reducing bacteria enrichment mediu...
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