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A culture method for inducing callus of Rosa roxburghii fruit and promoting the accumulation of its active substances

A callus induction and culture method technology, applied in the field of plant tissue culture, can solve the problems of unexplored callus culture method and callus induction technology etc.

Active Publication Date: 2021-08-31
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the plant tissue culture research of Rosa roxburghii has not involved the callus induction technology using the fruit as explants, nor has it explored the callus culture method aimed at promoting the accumulation of active substances in Rosa roxburghii.

Method used

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  • A culture method for inducing callus of Rosa roxburghii fruit and promoting the accumulation of its active substances
  • A culture method for inducing callus of Rosa roxburghii fruit and promoting the accumulation of its active substances
  • A culture method for inducing callus of Rosa roxburghii fruit and promoting the accumulation of its active substances

Examples

Experimental program
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Effect test

Embodiment 1

[0025] The present embodiment provides the determination of the optimum medium and culture conditions for inducing the active substance accumulation of Rosa roxburghii fruit callus, and the steps are as follows:

[0026] 1. Establishment of callus induction and culture system of Rosa roxburghii fruit

[0027] (1) Selected and planted in Guizhou University Germplasm Resource Garden Rosa roxburghii ( Rosa Roxburghii Tratt.) 'Gui Nong No. 5' was used as the test material, and the thorn pear fruit with no pests and diseases and good growth 80-90 days after flowering was selected as the explant.

[0028] (2) Preparation of medium for callus induction of Rosa roxburghii fruit. According to the instructions of the medium, weigh the WPM solid medium powder and dissolve it in distilled water, then add 30g·L -1 Stir the sucrose to make it fully dissolve, and then use a pipette gun to draw the prepared plant growth regulator mother solution in the obtained solution, the concentration ...

Embodiment 2

[0064] This embodiment adopts the callus subcultured 4 times in Example 1 and the culture conditions, and cultivates it in the optimal medium for 28 days to carry out the accumulation of its active substances. After measuring the vitamin C, total phenols, The contents of total flavonoids and total triterpenes are respectively 48 mg·100 g -1 , 1313.32 mg . 100g -1 , 458.90 mg 100g -l and 1928.99 mg·100g -1 .

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Abstract

The invention discloses a cultivation method for inducing callus of Rosa roxburghii fruit and promoting the accumulation of active substances thereof, belonging to the technical field of plant tissue culture. The method comprises the following steps: (1) collecting Rosa roxburghii fruits at a suitable period; (2) processing and disinfecting Rosa roxburghii fruits; (3) inoculating the sterilized explants in a callus induction medium; ( 4) After 3-4 weeks of culture, the callus of Rosa roxburghii fruit induced in step (3) was proliferated; (5) The following medium and culture conditions were used to detect the main active substances in the callus of Rosa roxburghii fruit Accumulation: MS+3.0‑4.0 mg·L ‑1 TDZ+0.1 mg·L ‑1 NAA+50‑60 g L ‑1 Sucrose / glucose + appropriate amount of agar, the pH value is 5.8‑6.0, the culture temperature is 25 ° C, the light time is 12 hours per day, and the light intensity is 10‑15 µmol m ‑2 ·s ‑1 .

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a culture method for inducing callus of Rosa roxburghii fruit and promoting the accumulation of its active substances. Background technique [0002] prickly pear ( Rosa Roxburghii Tratt.) belongs to the Rosaceae Rosa fruit tree, a perennial shrub. The fruit contains rich vitamin C, phenols, flavonoids, triterpenes and other nutrients and active substances. Main target components and action molecules. Vitamin C (Vc), namely ascorbic acid (L-ascorbic acid), is a compound necessary for the human body to maintain the normal growth and development of human beings. It has the functions of scavenging free radicals, anti-aging, anti-oxidation, and regulating human body functions; Polyphenols, flavonoids and triterpenoids can remove active oxygen and free radicals in the human body, improve the body's antioxidant capacity, enhance immunity, protect the cardiova...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/005
Inventor 安华明房洪舟鲁敏马文涛
Owner GUIZHOU UNIV
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