Unlock instant, AI-driven research and patent intelligence for your innovation.
Application of radix puerariae, radix scutellariae and rhizoma coptidis preparation in inhibition of novel coronavirus
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of pueraria chinensis and coronavirus, which is applied in the field of medicine to achieve the effects of good resistance, promotion of health recovery, and removal of pathogens
Inactive Publication Date: 2021-02-12
广西壮族自治区花红药业集团股份公司
View PDF0 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0007] At present, there are many drug studies on the new coronavirus. Although no antiviral drugs have been found to be effective in strict "randomized, double-blind, placebo-controlled studies", some drugs may have certain therapeutic effects through clinical observation studies.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
[0055] Table 1: Drug name, experimental concentration and grouping
[0056]
[0057] (2) In a sterile 96-well culture plate, add 100 μL of Vero E6 cells at a concentration of 2×10 cells / mL to each well, and incubate at 37° C. and 5% CO for 24 hours;
[0058] (3) The monolayer cells of the 96-well plate were washed once with PBS, and 100 μL of 2-fold serially diluted drug was added to each well. Add an equal volume of culture medium to each well of the blank control group and normal cell group, and incubate at 37°C for 3 days;
[0059] (4) Add 20 μL of MTT solution with a concentration of 5 mg / mL to each well and continue to incubate for 4 hours. Discard the supernatant, add 100 μL DMSO to each well, and shake at low speed for 5 minutes to fully melt the crystals;
[0064] Embodiment 2 test drug antiviral experiment
[0065] (1) Experimental method:
[0066] (1) Test drug:
[0067] Table 2: Drug name, experimental concentration and grouping
[0068]
[0069]
[0070] (2) In a sterile 96-well culture plate, add 100 μL of VeroE6 cells at a concentration of 2×105 cells / mL to each well, and incubate at 37°C and 5% CO2 for 24 hours;
[0071] (3) 100 μL / well of 100 TCID50 virus solution was added to the culture plate experimental group and the virus control group, and adsorbed for 2 hours at 37° C. and 5% CO2 incubator;
[0072] (4) After 2 hours, the cell culture solution in the 96-well culture plate was discarded; the test drug was diluted to each concentration in Table 1, and each concentration was repeated in 3 wells, and 100 μl / well of the above drug solution was added; at the same time, a cell control, Blank control (solvent control) and virus control (negative control); cells were incubated at 37°C, 5% CO2 incubator for 3 days; ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention discloses a method for reducing in-vitro cytopathy caused by novel coronavirus by using a radix puerariae, scutellaria baicalensis and coptis chinensis preparation, and application of the radix puerariae, scutellaria baicalensis and coptis chinensis preparation in preparation of a medicine for inhibiting novel coronavirus infection, wherein the novel coronavirus is SARS-CoV2, the dosage forms of the radix puerariae, scutellaria baicalensis and coptis chinensis preparation comprise pills, tablets, capsules, granules, suspensions or oral liquids, the cells are Vero E6, and the radix puerariae, scutellaria baicalensis and coptis chinensis preparation comprises the following raw materials: 1000 g of radix puerariae, 375 g of scutellaria baicalensis, 375 g of coptis chinensis and250 g of radix glycyrrhizae preparata.
Description
technical field [0001] The invention belongs to the field of medicine, and in particular relates to the application of Gegenqinlian preparation in inhibiting novel coronavirus. Background technique [0002] The 2019 novel coronavirus is a newly discovered coronavirus, officially named SARS-CoV-2 by the International Committee on Taxonomy of Viruses. [0003] Patients with novel coronavirus pneumonia mainly present with fever, dry cough, and fatigue. Some patients have decreased or lost sense of smell and taste as the first symptom, and a few patients have symptoms such as nasal congestion, runny nose, sore throat, conjunctivitis, myalgia, and diarrhea. Severe patients often develop dyspnea and / or hypoxemia one week after the onset, and severe cases can rapidly progress to acute respiratory distress syndrome, septic shock, difficult-to-correct metabolic acidosis, coagulation dysfunction and multiple organ failure etc. A very small number of patients may also have manifesta...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More