Micropterus salmoides myocardial fibroblast cell line and application

Abstract

The invention belongs to the technical field of biology, and particularly relates to a micropterus salmoides myocardial fibroblast cell line and application. According to the cell line, aiming at thecharacteristics of micropterus salmoides heart tissue cells, brain tissue cells are separated by adopting a collagenase I and pancreatin combined digestion method, primary culture is performed, the micropterus salmoides myocardial fibroblast cell line is successfully constructed and is continuously passed to more than 60 generations, a large number of micropterus salmoides heart-derived cells canbe provided, and the cells can maintain a good growth state and can be cryopreserved. The micropterus salmoides myocardial fibroblast cell line has sensitivity to various fish viruses, cytopathy is generated after virus inoculation, the cell line can be directly applied to pathogen characteristic research and vaccine development, and the micropterus salmoides myocardial fibroblast cell line can also be used for expressing exogenous genes for gene function research.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a largemouth bass myocardial fibroblast cell line and its application. Background technique [0002] The scientific name of California perch is large mouth bass (Micopterus salmoides), which belongs to Perciformes, Ceutrarchidae, and Micropterus. It is native to California, USA, and is a typical carnivorous freshwater fish. , is one of the most important game fish species in the United States. my country introduced it from abroad in the 1980s. It has been proved by trial breeding that the fish has the advantages of fast growth, less disease, low temperature resistance, less meat and thorns, delicious taste, and rich nutrition. It has become the main fish species for freshwater aquaculture in my country. One of them, and in recent years it has been honored as the "fifth family fish". California perch can achieve high yield and high efficiency in most of the waters in my country in mo...

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Problems solved by technology

[0004] At present, there is no report on the establishment of largemouth bass cell lines. Different virus-sensitive cell lines are different. At present, the viruses that are more harmful to largemouth bass include iridovirus swollen genus and iridescent virus ranavirus genus. virus, neuronecrosis virus, etc., and the cells commonly used to isolate these viruses are mainly carp epithelial cells (EPC), fat-headed carp muscle cell lines (FHM) and other fish cell lines with large differences in species, although some perch-derived The virus can replicate on these cells and produce CPE, but the sensitivity is not high enough and the virus titer is not high, which is extremely unfavorable for the isolation, identification, etiology research and vaccine development of the virus; The institute and Sun Yat-sen University respectively established the mandarin fish brain tissue cell line (CPB) and the mandarin fish larvae cell line (MFF-1), both of which showed sensitivity to several major viruses derived from perch

However, since CPB and MFF-1 were both established for the development of vaccines for mandarin fish viral diseases, cell lines derived from other fish are also foreign substances to sea bass, so cell lines from other sources were used for production Vaccines will produce a non-specific immune response, which will affect the immune effect of the vaccine. Therefore, cell lines from the host should be used as much as possible in vaccine research and production.

So far, there has been no report on the cardiac fibroblast cell line of largemouth bass. The establishment of the cardiac fibroblast cell line of largemouth bass established by the present invention lays a solid foundation for the later establishment of cell lines of other tissues of sea bass and virus research

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