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Metarhizium preparation for promoting banana plant growth and preventing and treating banana wilt

A technology of banana fusarium wilt and metarhizium anisopliae, applied in the field of microorganisms, can solve the problems of restricting the industrial production of bananas and the shortness of banana plants, and achieve the effect of no toxic side effects, simple method, and promotion of plant growth

Inactive Publication Date: 2021-03-02
重庆谷百奥生物研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, at present, the banana plants are short, which restricts the industrialized production of bananas.

Method used

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  • Metarhizium preparation for promoting banana plant growth and preventing and treating banana wilt
  • Metarhizium preparation for promoting banana plant growth and preventing and treating banana wilt
  • Metarhizium preparation for promoting banana plant growth and preventing and treating banana wilt

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Effect of Metarhizium anisopliae CQMa421 Oil Suspension Concentrate on Promoting Growth of Banana Indoor Potted Plants

[0027] Banana variety: Brazilian banana (purchased from Guangdong Yitong Agricultural Development Co., Ltd.);

[0028] Test treatment: Banana seedlings were transplanted into pots. After the banana seedlings survived, the roots were irrigated with 200-fold dilutions, 400-fold dilutions, and 800-fold dilutions of Metarhizium anisopliae CQMa421 oil suspension concentrate, and the roots were irrigated with clear water. The treatment was the control, with 500ml of plants in each pot, 30 pots for each treatment, and repeated 3 times; after the test treatment, measure the height of the plants 40 days later, pull out the plants and wash them, and weigh the banana plants on the ground after drying them in an oven. Part and root dry weight, calculate the average value, and adopt SPSS and Excel statistical analysis data, the result is shown in Table 1.

[0029...

Embodiment 2

[0034] Verification of the effect of Metarhizium anisopliae CQMa421 on banana Fusarium wilt in indoor plate test

[0035] experimental method

[0036] Fusarium oxysporum fs.p.cubense (FOC) was provided by the School of Life Sciences, Chongqing University.

[0037] Using the flat plate confrontation culture method, move the tested banana fusarium wilt bacteria cake (9 mm in diameter) to the center of the plate, inoculate CQMa421 bacteria at 2 cm around, and cultivate in a biochemical incubator at 27 ° C. Each treatment is repeated 3 times, and the average value as the test result;

[0038] When the mycelium of the control group covered the plate, the diameter of the colony and the diameter of the inhibition zone were measured by the cross method, and the average value was calculated, and the inhibition rate of tomato anthracnose was calculated according to the following formula. The results are shown in Table 2.

[0039]

[0040] Table 2 Inhibitory activity against Fusariu...

Embodiment 3

[0044] Control effect of CQMa421 oil suspension of Metarhizium anisopliae CQMa421 on banana fusarium wilt in indoor potted plants

[0045] Pathogen: Fusa rium oxysporum fs.p.cubense (FOC) provided by the School of Life Sciences, Chongqing University

[0046] Banana variety: Brazilian banana (purchased from Guangdong Yitong Agricultural Development Co., Ltd.);

[0047] Pathogen preparation: inoculate Fusarium oxysporum on potato agarose (PDA) medium, culture at 25°C for 7 days, punch 3 fungus cakes at the edge of the colony with a puncher, and inoculate the fungus cakes into 100ml potato dextrose liquid culture solution Medium, 25°C, 180r / min shaking culture for 5 days, filter with 4 layers of sterile lens paper, adjust the spore concentration to 1×10 with a hemocytometer 6 Spores / ml for later use.

[0048] Test treatment: before transplanting banana seedlings into pots, soak the roots in 200-fold, 400-fold, and 800-fold dilutions of Metarhizium anisopliae CQMa421 oil suspens...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a Metarhizium preparation for promoting banana plant growth and preventing and treating banana wilt. The Metarhizium preparation can significantly promote the growth of banana plants, and has a good prevention effect on banana wilt.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Metarhizium anisopliae preparation for promoting the growth of banana plants and preventing and treating banana wilt. Background technique [0002] Banana (MusaAAA Group) is known as sugarcane and plantain in ancient times, also known as Chinese dwarf banana, Tianbao banana, and pink banana. It is a perennial plant of the Musa family. Bananas are native to tropical Asia, and are now widely distributed in tropical and subtropical regions between north and south latitudes of 30°. At present, there are more than 100 countries and regions in the world that cultivate bananas ("Bananas and their health functions", Zhu Yu'an, Chinese Food and Nutrition , No. 3, 2006, 1-4 lines of the first paragraph of the left column on page 51, published on December 31, 2006). [0003] The banana plant is a large plant, emanating from the rhizome, forming a 3-6 meter high false s...

Claims

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Application Information

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IPC IPC(8): A01N63/30A01P21/00A01P3/00A01M17/00A01G13/00A01G22/05A01G17/00
CPCA01G13/00A01G17/005A01M17/002A01G22/05A01N63/30
Inventor 夏玉先蒙玲玲彭国雄
Owner 重庆谷百奥生物研究院有限公司
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