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A kind of animal bifidobacterium milk subspecies bp026 and its application

A technology of animal bifidobacteria, BP026, applied in the direction of bifidobacteria, applications, bacteria, etc., to achieve remarkable effects, promote sleep, and prolong sleep duration

Active Publication Date: 2022-04-12
ZHEJIANG MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the intestinal flora will absorb and utilize the nutrients ingested by the body, produce metabolic small molecules such as 5-HT, GABA, etc., and feed back to the central nervous system and biological clock genes through the vagus nerve, and then realize gut-brain communication, but so far no animal-derived dual Related reports of Mycobacterium species improving sleep

Method used

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  • A kind of animal bifidobacterium milk subspecies bp026 and its application
  • A kind of animal bifidobacterium milk subspecies bp026 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1. Isolation and Purification of Animal Bifidobacterium Milk Subspecies

[0031] The feces of healthy people were used as samples, inoculated in a solid medium suitable for the growth of bifidobacteria, and anaerobically cultured at 37°C for 48 hours; white colonies with smooth surfaces were picked and inoculated into a liquid medium suitable for the growth of bifidobacteria. Anaerobic culture was carried out at 37°C for 48 hours; the genome was extracted, 16S rDNA was amplified by PCR, sequenced and compared, and Bifidobacterium animalis subsp. lactis BP026 was discovered and preserved.

Embodiment 2

[0032] Embodiment 2. the acid resistance characteristic of bifidobacterium animalis milk subspecies BP026

[0033] Bifidobacterium animalis subsp. lactis BP026 was suspended in PBS with pH of 1.0, 2.0, 3.0, 4.0, 7.0, cultured at 37°C for 1h, 2h, 3h, 4h, and the viable bacteria rate was determined by plate counting method. See Table 1 for the live bacteria rate of Bifidobacterium animalis milk subsp. BP026 at different pH conditions and at different time points.

[0034] Table 1. Viability rate of Bifidobacterium animalis milk subspecies BP026 under acidic conditions

[0035] Viable bacteria rate (%) 1h 2h 3h 4h pH=1 0 0 0 0 pH=2 12.00±1.61 0.01±0.00 0.01±0.00 0.01±0.00 pH=3 94.42±4.03 86.05±24.42 88.37±21.31 61.86±4.49 pH=4 90.70±6.08 92.56±21.36 86.05±3.51 76.74±13.31 pH=7 98.60±8.53 99.53±7.68 85.27±6.35 79.53±9.77

[0036] Note: 3 parallels were set up in this experiment, and the data are mean ± SD.

Embodiment 3

[0037] Example 3. Bile-resistant properties of Bifidobacterium animalis milk subspecies BP026

[0038] Bifidobacterium animalis milk subsp. BP026 was suspended in PBS containing 0%, 0.3%, 0.6% and 1% ox bile, cultured at 37°C for 1h, 2h, 3h, 4h, and the viable bacteria rate was determined by plate counting method. See Table 2 for the live bacteria rate of Bifidobacterium animalis milk subspecies BP026 at different bile concentrations and at different time points.

[0039] Table 2. Viable bacteria rate after bile treatment of Bifidobacterium animalis milk subspecies BP026

[0040] Viable bacteria rate (%) 1h 2h 3h 4h 0% bile 94.09±19.73 87.50±30.31 83.99±10.02 75.07±11.42 0.3% bile 24.75±1.77 2.13±0.26 1.27±0.42 1.77±0.26 0.6% bile 85.91±6.13 8.49±1.56 6.87±3.01 2.09±0.36 1% bile 99.45±24.23 36.52±21.44 4.60±0.35 1.01±0.22

[0041] Note: 3 parallels were set up in this experiment, and the data are mean ± SD.

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Abstract

The invention discloses a Bifidobacterium animalis subsp. lactis BP026 and an application thereof, which are preserved in the General Microbiology Center of the China Microorganism Culture Collection Administration Committee, and the preservation time is August 6, 2020, and the preservation number is It is CGMCC No.20495. The strain has the effect of improving sleep or promoting sleep, and can be used for preparing sleep-improving food, sleep-improving health food and sedative and tranquilizing medicine. In animal experiments, it can significantly shorten the sleep latency and prolong the sleep duration, improve the sleep quality of mice, significantly shorten the sleep latency and prolong the sleep duration, and improve the sleep quality of mice. The effect is remarkable and has broad application prospects.

Description

technical field [0001] The invention relates to the technical field of microbial products, in particular to Bifidobacterium animalis subsp. lactis BP026 and an application thereof. Background technique [0002] Sleep problems are an important problem affecting human health, mainly reflected in difficulty in falling asleep, short sleep time, easy to wake up and early awakening. According to incomplete statistics, sleep problems affect about 15% of the Chinese population, and this proportion is higher in the United States, exceeding 30%. Insufficient sleep will have a negative impact on people's work and life during the day, such as fatigue, lethargy, headache, etc. Long-term sleep problems may also lead to other diseases such as depression, obesity, diabetes and heart disease, and even social problems such as suicide. At present, the treatment of insomnia is mainly to take artificial synthetic drugs, but synthetic drugs have serious drug dependence, cognitive and motor impai...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L33/135A61K35/745A61P25/20C12R1/01
CPCC12N1/20A23L33/135A61K35/745A61P25/20A23V2002/00A23V2400/515A23V2200/31
Inventor 俞盈么春艳
Owner ZHEJIANG MEDICAL COLLEGE
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