Application of bacteroides fragilis in preparation of medicine for treating inflammatory bowel diseases

A technology for Bacteroides fragilis and inflammatory bowel disease, applied in the field of biomedicine

Inactive Publication Date: 2021-03-12
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention found for the first time that Bacteroides fragilis ATCC25285 can effectively treat inflammatory bowel disease, especially ulcerative colitis, by regulating the homeostasis of intestinal flora and enhancing intestinal regulatory immunity. Exploring the role of Bacteroides fragilis ATCC25285 on intestinal inflammation from the perspective of intestinal immunity has not been reported yet

Method used

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  • Application of bacteroides fragilis in preparation of medicine for treating inflammatory bowel diseases
  • Application of bacteroides fragilis in preparation of medicine for treating inflammatory bowel diseases
  • Application of bacteroides fragilis in preparation of medicine for treating inflammatory bowel diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Cultivation of Bacteroides fragilis ATCC 25285

[0026] 1. Training method

[0027] TSA + 10% sterile defibrinated sheep blood, cultured for 48 hours at 37°C under strict anaerobic conditions.

[0028] The formula of 1L TSA medium is as follows: tryptone...15g

[0029] Soy peptone...5.0g

[0030] NaCl……………5.0g

[0031] Agar powder…………15g

[0032] .

[0033] Weigh 40g of this product, dissolve it in 1000ml of distilled water with heating and stirring, divide into Erlenmeyer flasks, and autoclave at 121°C for 15 minutes. Cool to about 50 degrees, add 10% sterile defibrinated sheep blood, shake and mix well, pour plate. Spread the bacteria on the plate and culture according to the condition.

Embodiment 2

[0035] Effect of Bacteroides fragilis ATCC 25285 in the treatment of colitis

[0036] Select healthy and mature SPF grade C57 / BL6 female mice, weighing 18-22g, purchased from Nanjing Qinglongshan Animal Breeding Farm, a total of 18 mice were randomly divided into 3 groups, 6 mice in each group, and all mice were adaptively fed for 1 day before the experiment. week.

[0037] 1. Grouping and administration of animals

[0038] Blank control group (NC group): Mice drank sterile water.

[0039] Colitis group (DSS group): the mice were given 3% DSS solution as drinking water, and after 7 days, they were replaced with normal drinking water, and 200 μL of sterile water was fed into the stomach every day, and they were killed after 3 days.

[0040] Administration group (DSS+B.fragilis group): the mice were given 3% DSS solution as drinking water, changed to normal drinking water after 7 days, and 200 μL of Bacteroides fragilis (containing live bacteria 10 6 -10 10 CFU), sacrificed ...

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Abstract

The invention relates to the field of biological medicines, and provides application of bacteroides fragilis in preparation of medicines for treating inflammatory bowel diseases. The bacteroides fragilis is bacteroides fragilis ATCC 25285. In-vivo experimental research finds that bacteroides fragilis ATCC 25285 can effectively regulate intestinal flora balance, enhance intestinal tract regulatoryimmunity and inhibit secretion of inflammatory cytokines for inflammatory bowel disease-ulcerative colitis, relieves intestinal inflammation, has no toxic or side effect, and has a great application potential in the aspect of treating inflammatory bowel disease.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of Bacteroides fragilis in treating inflammatory bowel disease. Background technique [0002] Inflammatory bowel disease (Inflammatory bowel disease, IBD) is a group of chronic intestinal inflammatory diseases of unknown cause, including ulcerative colitis (Ulcerativecolitis, UC) and Crohn's disease (Crohn's disease, CD). The former, also known as nonspecific ulcerative colitis, is an inflammation of the rectum and colon of unknown cause, and the lesions are mainly limited to the mucosa and submucosa of the large intestine. The pathogenesis of inflammatory bowel disease has not been elucidated, and it is currently believed to be caused by excessive innate or acquired immune responses to intestinal commensal microorganisms in genetically susceptible individuals. [0003] There is still no effective treatment for inflammatory bowel disease. At present, its therapeutic dr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/741A61P1/00A61P1/04A61P29/00
CPCA61K35/741A61P1/00A61P1/04A61P29/00
Inventor 邢莹莹奚涛范梦慧
Owner CHINA PHARM UNIV
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