Preparation method of single-phase cross-linked sodium hyaluronate gel
A technology for cross-linking hyaluronic acid and sodium hyaluronate, applied in the field of pharmaceutical preparation, can solve the problems of unstable quality control, influence of gel properties, influence of product quality, etc., so as to reduce the risk of cross-contamination, ensure quality, reduce The effect of human manipulation
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[0023] A preparation method of single-phase cross-linked sodium hyaluronate gel, which comprises the following steps:
[0024] S1. Add the cross-linking agent BDDE and NaOH solution in a centrifuge tube at a mass ratio of 1:8-1:10, and then put nitrogen into it and mix it in a centrifuge to obtain a NaOH mixture containing the cross-linking agent BDDE. solution, spare;
[0025] S2. Mix sodium hyaluronate and NaOH solution with pH>13 in a polytetrafluoroethylene cup with a cover at a mass ratio of 1:6-1:8, stir and dissolve, cover the cup and place it in a three-dimensional Mix in a mixer for 1-2 hours to obtain a sodium hyaluronate solution; wherein, the molecular weight of the sodium hyaluronate is 2.1-2.7 MDa;
[0026] S3. Add all the NaOH mixed solution prepared in step S1 into the sodium hyaluronate solution, mix well, and then pass nitrogen gas, so that the air in the cup is completely replaced by nitrogen gas, then cover the cup, and put it in a constant temperature wat...
Embodiment 1
[0030] A preparation method of single-phase cross-linked sodium hyaluronate gel, which comprises the following steps:
[0031] S1. Add 700 μL of cross-linking agent BDDE and 5 g of NaOH solution to a centrifuge tube, pass nitrogen gas into it, and mix in a centrifuge to obtain a NaOH mixed solution containing cross-linking agent BDDE, which is set aside;
[0032] S2. Take 10 g of sodium hyaluronate powder with a molecular weight of 2.1 MDa and dissolve it in 90 g of 0.3 mol / L sodium hydroxide solution in a PTFE beaker, then stir and dissolve it on a three-dimensional mixer at a speed of 70 rpm;
[0033] S3. After fully stirring and dissolving, add NaOH mixed solution, stir evenly, inject nitrogen gas into the cup and immediately cover the cup and transfer it to a constant temperature water bath at 45°C for 3 hours to obtain sodium hyaluronate gel;
[0034] S4. Cut the gel into small pieces of 0.5×0.5cm, add 9g of 3mol / L hydrochloric acid and 160g of PBS solution, place it on a...
Embodiment 2
[0037] A preparation method of single-phase cross-linked sodium hyaluronate gel, which comprises the following steps:
[0038] S1. Add 700 μL of cross-linking agent BDDE and 5 g of NaOH solution to a centrifuge tube, pass nitrogen gas into it, and mix in a centrifuge to obtain a NaOH mixed solution containing cross-linking agent BDDE, which is set aside;
[0039] S2. Take 10 g of sodium hyaluronate powder with a molecular weight of 2.5 MDa and dissolve it in 90 g of 0.3 mol / L sodium hydroxide solution in a PTFE beaker, then stir and dissolve it on a three-dimensional mixer at a speed of 70 rpm;
[0040] S3. After fully stirring and dissolving, add NaOH mixed solution, stir evenly, inject nitrogen gas into the cup and immediately cover the cup and transfer it to a constant temperature water bath at 50°C for 2 hours to obtain sodium hyaluronate gel;
[0041]S4. Cut the gel into small pieces of 0.5×0.5cm, add 9g of 3mol / L hydrochloric acid and 160g of PBS solution, place it on a ...
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