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A strain of Lactobacillus rhamnosus capable of preventing and relieving ulcerative colitis and its application

A technology for Lactobacillus rhamnosus and ulcerative colitis, applied in the field of functional microorganisms, which can solve problems such as individual differences

Active Publication Date: 2022-08-02
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Lactobacillus is the most common probiotic, safe and edible, without side effects. A large number of studies have shown that some special Lactobacillus strains have good anti-inflammatory and intestinal flora effects, but this effect is obvious in Lactobacillus individual differences

Method used

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  • A strain of Lactobacillus rhamnosus capable of preventing and relieving ulcerative colitis and its application
  • A strain of Lactobacillus rhamnosus capable of preventing and relieving ulcerative colitis and its application
  • A strain of Lactobacillus rhamnosus capable of preventing and relieving ulcerative colitis and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Lactobacillus rhamnosus CCFM1128 has good tolerance to simulated gastrointestinal fluid

[0050] The cryopreserved Lactobacillus rhamnosus CCFM1128 was inoculated into mMRS medium (MRS medium + 0.05% cysteine ​​hydrochloride), cultured anaerobically at 37°C for 48h, and then subcultured with mMRS medium for 2~ After three times, a culture solution of Lactobacillus rhamnosus CCFM1128 was obtained.

[0051] Take 1 mL of the culture solution of Lactobacillus rhamnosus CCFM1128, mix it with 9.0 mL of pH 2.5 artificial simulated gastric juice (mMRS medium containing 1% pepsin, pH = 2.5), and culture it anaerobically at 37 °C for 0 h, Samples were taken at 0.5h, 1h, 2h and 3h, and the plate colonies were counted with mMRS agar medium for pouring and culture, and the number of viable bacteria was determined and the survival rate was calculated. The survival rate is the ratio of the logarithmic number of viable bacteria at the time of sampling to the logarithmic val...

Embodiment 2

[0058] Example 2: Lactobacillus rhamnosus CCFM1128 has no toxic and side effects on C57BL / 6J mice

[0059] Lactobacillus rhamnosus CCFM1128 was resuspended in 3g / 100mL sucrose solution to make the concentration of 5.0×10 9 CFU / mL bacterial suspension. Take 6 healthy male C57BL / 6J mice with a weight of about 14-16g, and after a week of acclimatization, the bacterial suspension of this concentration is given once a day (0.2mL for each gavage), observed for one week, and the death and body weight are recorded. .

[0060] The results of these tests are listed in Table 3. These results show that the feeding concentration of 5.0 × 10 9 Lactobacillus rhamnosus CCFM1128 at CFU / mL did not cause significant effects on mice, no significant changes in body weight, and no death phenomenon. The mice showed no obvious pathological symptoms.

[0061] Table 3 Changes in body weight and death of mice

[0062] time (days) 1 2 3 4 5 6 7 Weight (g) 19.22±0.10 19.40±0....

Embodiment 3

[0064] Example 3: Effects of Lactobacillus rhamnosus CCFM1128 on disease symptoms in UC mice

[0065] Thirty healthy male C57BL / 6J mice weighing 14-16g were selected, acclimated to the environment for 1 week, 6 mice in each group, and randomly divided into 5 groups: blank group, model group, drug group, Lactobacillus rhamnosus CCFM1128 intervention group (CCFM1128), Lactobacillus rhamnosus FNMGEL5-1 control group (FNMGEL5-1). The dose of gavage bacterial suspension is 5.0×10 9 CFU / mL, resuspended in 3% (w / v) sucrose solution. Dextran sulphate sodium (DSS) was added to drinking water at a final concentration of 2.5 g / 100 mL (ie 2.5% (w / v)) at week 5 to induce colitis in mice. The groups and treatment methods of experimental animals are shown in Table 4:

[0066] Table 4 Design of animal experiments

[0067]

[0068] During the fifth week, during the modeling period (during DSS treatment), the mice were weighed regularly every day and the percentage change was calculated....

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Abstract

The invention discloses a strain of Lactobacillus rhamnosus capable of preventing and relieving ulcerative colitis and its application, belonging to the technical field of functional microorganisms. Lactobacillus rhamnosus CCFM1128 can tolerate the human gastrointestinal environment, significantly improve body weight loss during ulcerative colitis, improve stool texture and blood in the stool, reduce colonic mucosal damage, reduce MPO activity, and reduce the pro-inflammatory factor TNF in the colon Produces ‑α, IL‑1β, IL‑6, IFN‑γ, reduces the content of pro-inflammatory factor IL‑6 in serum, and upregulates the gene transcription levels of colon tight junction-related proteins Claudin‑3, ZO‑1, ZO‑2 and Occludin , up-regulated the transcription level of the antimicrobial peptide CRAMP in the colon, increased the content of short-chain fatty acids in feces, increased the diversity of intestinal flora, and increased the abundance of short-chain fatty acid-producing bacteria Coprococcus in the intestine.

Description

technical field [0001] The invention relates to a Lactobacillus rhamnosus strain capable of preventing and relieving ulcerative colitis and its application, in particular to the application of Lactobacillus rhamnosus CCFM1128 in the preparation of functional bacterial agents, food and / or medicine, belonging to functional microorganisms technical field. Background technique [0002] In Western countries, Inflammatory Bowel Disease (IBD) is a common autoimmune disease of the digestive system, and its incidence is as high as 5 to 12 patients per 100,000 population. With the increasing pressure of Chinese people's life and changes in dietary patterns, the incidence of inflammatory bowel disease in China has increased by more than 15 times compared with 20 years ago. Treatment methods were explored in depth. Ulcerative colitis (UC) is a type of IBD with the main clinical manifestations of chronic or subacute diarrhea, mucus, pus and blood in the stool and abdominal pain. UC is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L33/135A61K35/747A61P1/04C12R1/225
CPCC12N1/20A23L33/135A61K35/747A61P1/04A23V2002/00A23V2400/175A23V2200/32A23V2200/3202
Inventor 王刚郑雨星王琳琳翟齐啸陆文伟崔树茂杨波毛丙永唐鑫赵建新张灏陈卫
Owner JIANGNAN UNIV
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