Application of Sphingomonas cucurbitus and its fermentation products in the control of rice bacterial diseases

A technology of sphingomonas and fermentation products, applied in the fields of bacteria, application, rice cultivation, etc., can solve the problem that there is no broad-spectrum biocontrol agent for the prevention and control of rice bacterial diseases, and achieve the goal of inhibiting the development of diseases Effect

Active Publication Date: 2021-09-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the pesticides on the market to prevent and control bacterial diseases mainly include inorganic copper, organic copper and thiazoles, but there is no broad-spectrum biocontrol agent for the prevention and control of rice bacterial diseases

Method used

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  • Application of Sphingomonas cucurbitus and its fermentation products in the control of rice bacterial diseases
  • Application of Sphingomonas cucurbitus and its fermentation products in the control of rice bacterial diseases
  • Application of Sphingomonas cucurbitus and its fermentation products in the control of rice bacterial diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] This example provides the inhibitory effect of Sphingomonasmelonis fermentation products on rice pathogenic bacteria. The specific operation is:

[0048] 1. Sphingomonasmelonis cultivation: the Sphingomonasmelonis (Sm) strain was routinely cultivated in a constant temperature incubator at 25° C., and the selected medium was LB medium.

[0049] 2. Preparation of Sm fermentation product: Pick a single colony of Sm vertically with an inoculation needle, place it in 50mL of LB medium for pre-culture, after 24 hours of cultivation, draw 10ml of bacterial liquid, centrifuge at 5000rpm for 5min, and discard the supernatant Then add the same amount of clear water to wash, oscillate and mix, then centrifuge at 5000rpm for 5min, add the same amount of clear water again and oscillate to mix, absorb 100μL of the mixed bacteria solution and add it to 60mL MA liquid medium for culture, MA culture The formula ratio of the base is inorganic salt solution (NH 4 h 2 PO 4 0.1%, KCl 0...

Embodiment 2

[0069] This example provides the control application of Sm bacteria and its fermentation product in rice growth substrate. The specific operation is:

[0070] 1. Treatment of rice germination and pathogenic bacteria inoculation: put the rice seeds in a glass petri dish, add a small amount of water (subject to the surface of the seeds), and then culture them in a constant temperature incubator at 25°C, and replace them every other day Clean water once, and after 2-3 days, the seeds will appear white, and the germination is completed at this time. Seeds after the completion of pregermination, select Bp and Bge that can cause seedling disease to do inoculation treatment respectively (10 5 CFU / mL, 6 hours), sterilized water was used as the uninfected blank control.

[0071] 2. Control effect of Sm bacteria and its fermentation products on seedlings infected by pathogenic bacteria: in MS plant culture medium, add Sm bacteria (10 3 、10 5 、10 7 CFU / mL) or Sm fermentation produc...

Embodiment 3

[0081] This example provides the control effect of Sm bacteria and its fermentation products on rice seeds. The specific operation is:

[0082] Paddy rice vernalization with reference to embodiment 2, the seed after vernalization completes, respectively uses Sm bacterium (10 3 、10 5 , 10 7 CFU / mL), Sm fermentation products (MR, 10, 50, 100 μg / mL) were soaked for 12 hours, and sterilized water was used as a blank control. Then, carry out pathogenic bacteria Bp or Bge treatment, rice transplanting and symptom observation with reference to embodiment 2. The observation results are shown in Table 9 and Table 10.

[0083] The control effect of table 9Sm bacterium and its fermented product soaking seed to infection Bp seedling

[0084]

[0085]

[0086] in, ** P<0.001.

[0087] The control effect of table 10Sm bacteria and its fermented product soaking seed to infection Bge seedling

[0088] deal with Seedling length (cm) root length (cm) blank control...

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Abstract

The invention discloses the application of Sphingomonas cucurbitus and its fermentation product in preventing and treating bacterial diseases of rice. Specifically including: the application of Sphingomonas cucurbitus and its fermentation products in the preparation of biological control agents for the prevention and treatment of rice bacterial diseases and the application of Sphingomonas cucurbitus and its fermentation products in the preparation of antibacterial agents Wait. By applying Sphingomonas cucurbitus and its fermentation product in rice planting, the disease development of various pathogenic bacteria infecting rice can be effectively inhibited, and different treatment methods can achieve relatively ideal control effects. At the same time, Sphingomonas cucurbitus and its fermentation products can also promote the growth of uninfected rice.

Description

technical field [0001] The invention relates to the technical field of rice disease prevention and control, in particular to the application of Sphingomonas cucurbitus and its fermentation product in the prevention and treatment of rice bacterial diseases. Background technique [0002] At present, the main bacterial diseases of rice are as follows: [0003] Rice bacterial blight is a bacterial disease caused by the infection of Xanthomonas oryzaepv.oryzae (Xoo), and it is also the subject of domestic quarantine. After sowing, it invades through the water holes and wounds of the leaves, and spreads through wind and rain, dew, irrigation water and the walking of managers. Common typical symptoms in the adult stage include leaf margin type, acute type, wilting type, midrib type and yellowing type. Among them, deep water irrigation, flooding, serial irrigation, flood irrigation, excessive nitrogen fertilizer, and excessive growth are all conducive to the occurrence of diseases...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/20A01P1/00C12N1/20A01C1/06A01C1/08A01G7/06A01G13/00A01G22/22C12R1/01
CPCA01C1/06A01C1/08A01G7/06A01G13/00A01G22/22A01N63/20C12N1/20
Inventor 王蒙岑松本春柰范小艳吕天星李蒙陈云马忠华杜永均王蔷薇虞云龙
Owner ZHEJIANG UNIV
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