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Cucurbit sphingomonas sp. and application of fermentation products of the cucurbit sphingomonas sp. in prevention and treatment of rice bacterial diseases.

A technology of sphingomonas and fermentation products, applied in the fields of application, bacteria, rice cultivation, etc., can solve the problem that there is no broad-spectrum biocontrol agent for the prevention and control of rice bacterial diseases, and achieve the goal of inhibiting the development of diseases Effect

Active Publication Date: 2021-03-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the pesticides on the market to prevent and control bacterial diseases mainly include inorganic copper, organic copper and thiazoles, but there is no broad-spectrum biocontrol agent for the prevention and control of rice bacterial diseases

Method used

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  • Cucurbit sphingomonas sp. and application of fermentation products of the cucurbit sphingomonas sp. in prevention and treatment of rice bacterial diseases.
  • Cucurbit sphingomonas sp. and application of fermentation products of the cucurbit sphingomonas sp. in prevention and treatment of rice bacterial diseases.
  • Cucurbit sphingomonas sp. and application of fermentation products of the cucurbit sphingomonas sp. in prevention and treatment of rice bacterial diseases.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] This embodiment provides the inhibitory effect of SPHINGOMONASMELONION fermentation product on raw bacteria in rice. The specific operation is:

[0048] 1.SPHINGOMONASMELONIS Culture: The Sphingomonasmelonis (SM) strain was cultured in a 25 ° C constant temperature incubator, and the medium selected medium was Lb medium.

[0049] 2. Preparation of SM fermentation products: Picking with the inoculation needle vertically, it is pre-cultured in 50 ml of LB medium, and after 24 hours, take the 10ml bacterial liquid, 5000 rpm Centrifuge 5 min Liquid, then add an equal amount of water cleaning, and mix with 5,000 rpm after mixing with 5000 rpm, then add equal amount of water and mix well, and absorb the mixed bacterial liquid 100 μL to 60 ml of MA liquid medium culture, MA culture The basis ratio of the base is inorganic salt solution (NH 4 Hide 2 PO 4 0.1%, KCl 0.02%, MGSO 4 · 7h 2 O 0.02%) 200X, glucose 10g, water 1L, after completion of the SM medium, at 24 ° C constant temper...

Embodiment 2

[0069] This example provides the prevention and treatment of SM bacteria and its fermentation product in rice growth matrix. The specific operation is:

[0070] 1. Rice germination and pathogen inoculation: Place rice seeds in a glass petri dish, add a small amount of water (with the surface of the seed surface), then placed in a 25 ° C constant temperature incubator, need to replace every other day Once a clear water, after 2-3 days, the seeds have a gentle oriented phenomenon. At this time, the germination is completed. The seeds after the germplasm are completed, select BP and BGE that can cause seedling diseases to be treated (10 5 CFU / mL, 6 hours), sterilized water as a non-infected blank control.

[0071] 2. Sm bacteria and its fermentation product for the prevention and treatment of pathogen infection seedlings: SM bacteria were added to MS plant medium (10 3 10 5 10 7 CFU / mL) or SM fermentation product (MR, 10, 50, 100 μg / mL), with the same volume of sterilized water...

Embodiment 3

[0081] This example provides the effectiveness of SM bacteria and its fermentation product treatment of rice seeds. The specific operation is:

[0082] Rice germination refers to the seeds after the germinated seed, separate SM bacteria (10 3 10 5 10 7 CFU / mL), SM fermentation product (MR, 10, 50, 100 μg / ml) made soaking treatment for 12 hours, sterilized water as a blank control. Then, referring to Example 2, a pathogen BP or BGE treatment, rice removal and symptom observation were performed. The observations are shown in Table 9 and Table 10.

[0083] Table 9SM bacteria and its fermentation product soaking effect on infection of BP seedlings

[0084]

[0085]

[0086] among them, ** P <0.001.

[0087] Table 10SM bacteria and its fermentation product Soaking effect on the control effect of infection BGE seedlings

[0088] deal with Seedling (cm) Root length (cm) Blank control 4.4 1.4 + BGE 1.0 **

0.5 **

+ SM bacteria (10 3 CFU / ml) + ...

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Abstract

The invention discloses cucurbit sphingomonas sp. and application of fermentation products of the cucurbit sphingomonas sp. in prevention and treatment of rice bacterial diseases. The application specifically comprises cucurbit sphingomonas sp. and application of fermentation products of the cucurbit sphingomonas sp. in prevention and treatment of rice bacterial diseases in the biological prevention and treatment agent, and cucurbit sphingomonas sp. and the application of the fermentation product thereof in preparation of a bacteriostatic agent and the like. By applying the cucurbit sphingomonas sp. and the fermentation product thereof in rice planting, the development of diseases caused by infection of rice by pathogenic bacteria can be effectively inhibited, and an ideal prevention and treatment effect is achieved in different treatment modes. Meanwhile, the cucurbitaceous sphingomonas and the fermentation product thereof can also play a role in promoting the growth of uninfected rice.

Description

Technical field [0001] The present invention relates to the field of prevention and treatment of rice diseases, and in particular, it is related to the use of melon sphingosine rage bacteria and its fermentation product in the prevention and treatment of rice bacterial disease. Background technique [0002] At present, the main bacterial disease of rice has the following: [0003] Rice white blight is a bacterial disease caused by xanthomonas oryzaepv.oryzae, XOO. It is also a domestic quarantine object. After sowing, the vapor hole and wound invading, borrowed by wind and rain, dew, irrigation water and management personnel. The typical symptoms commonly visible in a plant period have foliage type, as well as acute, witherent, medium pulse and yellowing. Among them, deep water irrigation, flood flooding, string, diffuse, nitrogen fertilizer, growing overwanging is conducive to disease. In particular, the most susceptible period of pregnancy period during the period of susceptibi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/20A01P1/00C12N1/20A01C1/06A01C1/08A01G7/06A01G13/00A01G22/22C12R1/01
CPCA01C1/06A01C1/08A01G7/06A01G13/00A01G22/22A01N63/20C12N1/20
Inventor 王蒙岑松本春柰范小艳吕天星李蒙陈云马忠华杜永均王蔷薇虞云龙
Owner ZHEJIANG UNIV
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