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Irradiation sterilization method of enzyme product

An irradiation sterilization and product technology, applied in the field of irradiation sterilization of enzyme products, can solve the problems of gelatin viscosity drop, protein loss, and high equipment cost, and achieve the effects of reducing microbial content, ensuring quality, and improving efficiency

Active Publication Date: 2021-03-16
湖南湘华华大生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, high-intensity irradiation treatment has a certain impact on the total saponin content, vitamin K2 content and enzyme activity in subtilisin, and in high-intensity irradiation treatment, there will be radiation odor
CN104773338A discloses a low-temperature high-dose-rate radiation sterilization method for gelatin anaerobic packaging. Although it solves the problems of gelatin protein loss and gelatin viscosity drop in radiation sterilization, its radiation dose is high, and the radiation time is long. Equipment higher cost

Method used

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  • Irradiation sterilization method of enzyme product
  • Irradiation sterilization method of enzyme product
  • Irradiation sterilization method of enzyme product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A radiation sterilization method for enzyme products, comprising the following steps:

[0028] S1 raw material pretreatment: take Bacillus subtilis protein kinase solution, add gelatin and protocatechuic acid, mix evenly by ultrasonic, freeze-dry in a vacuum freeze dryer to obtain freeze-dried powder of enzyme products;

[0029] S2 Electron beam irradiation treatment: Take the freeze-dried powder of S1 enzyme products, vacuumize and seal, pre-cool at 4°C for 1 hour, put it in a special metal tray for electron beam irradiation, and perform electron speed irradiation treatment to obtain radiation Irradiated enzyme products;

[0030] The electron beam output energy is 5MeV, the beam current power is 15.0kW, the power scanning frequency is 200pps, the transmission speed of the radiation tray under the scanner beam is 200mm / s, and the effective absorbed dose of electron beam irradiation is 3kGy; the sterilization time is 10min, obtain the enzyme product after irradiation; ...

Embodiment 2

[0035] A radiation sterilization method for enzyme products, comprising the following steps:

[0036] S1 raw material pretreatment: take Bacillus subtilis protein kinase solution, add gelatin and protocatechuic acid, mix evenly by ultrasonic, freeze-dry in a vacuum freeze dryer to obtain freeze-dried powder of enzyme products;

[0037] S2 electron beam irradiation treatment: Take the freeze-dried powder of S1 enzyme products, vacuumize and seal, pre-cool at 0°C for 2 hours, put it in a special metal tray for electron beam irradiation, and carry out electron speed irradiation treatment to obtain radiation Irradiated enzyme products;

[0038] The electron beam output energy is 7MeV, the beam current power is 16kW, the power scanning frequency is 250pps, the transmission speed of the radiation tray under the scanner beam is 100mm / s, the effective absorbed dose of electron beam irradiation is 4kGy; the sterilization time is 15min , to obtain irradiated enzyme products;

[0039] ...

Embodiment 3

[0043] A radiation sterilization method for enzyme products, comprising the following steps:

[0044] S1 raw material pretreatment: take Bacillus subtilis protein kinase, add gelatin and protocatechuic acid, mix evenly by ultrasonic, and freeze-dry in a vacuum freeze dryer to obtain freeze-dried powder of enzyme products;

[0045] S2 Electron beam irradiation treatment: take the freeze-dried powder of S1 enzyme products, vacuumize and seal, pre-cool at 4°C for 4 hours, put it in a special metal tray for electron beam irradiation, and perform electron speed irradiation treatment to obtain radiation Irradiated enzyme products;

[0046] The output energy of the electron beam is 8MeV, the beam current power is 17kW, the power scanning frequency is 300pps, the transmission speed of the radiation tray under the scanner beam is 150mm / s, the effective absorbed dose of electron beam irradiation is 5kGy; the sterilization time is 17min , to obtain irradiated enzyme products;

[0047] ...

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Abstract

The invention discloses an irradiation sterilization method of an enzyme product. According to the technical method, an enzyme product solution is subjected to pretreatment and electron beam irradiation treatment so as to achieve the purpose of sterilization. According to the method, the sterilization completeness can be ensured, meanwhile, the vacuum stability of the enzyme product in the irradiation sterilization process can be ensured, the problem that irradiation peculiar smell is generated in the irradiation treatment of the enzyme product can be effectively solved, soy isoflavone, enzymeactivity and methionine content of the enzyme product are not obviously influenced, the number of viable bacteria such as bacillus natto, coliform and staphylococcus aureus can be effectively reduced, the irradiation time is shortened, and the cost is reduced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a radiation sterilization method for enzyme products. Background technique [0002] At present, sterilization techniques include moist heat sterilization, dry heat sterilization, radiation sterilization, etc. Radiation sterilization is an effective method to kill microorganisms on most substances by ionizing radiation. It is suitable for biologically active and thermally unstable products, especially for the disinfection and sterilization of tissue materials, medicine and medical supplies, ensuring their biosecurity. The rays used for sterilization include electron beams, X-rays and gamma rays, etc., which can control the growth of microorganisms or kill microorganisms in specific ways. However, there is usually a certain amount of moisture inside the biological sample. In a vacuum state, the moisture in the biological sample will rapidly expand and transform into water vapor. The ...

Claims

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Application Information

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IPC IPC(8): A61L2/08A61K41/17
CPCA61L2/007A61L2202/21A61K41/17
Inventor 邹伟权罗志平王梦玲
Owner 湖南湘华华大生物科技有限公司
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