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Tricholoma matsutake and method for producing ergothioneine with same

A technology of ergothioneine and matsutake mushrooms, which is applied in the production of ergothioneine and matsutake mushrooms, can solve the problems of rare amino acid-ergothioneine research, no comprehensive utilization of ergothioneine and matsutake polysaccharides, etc., and achieve low cost , high output, and the effect of increasing output

Active Publication Date: 2021-03-16
BLOOMAGE BIOTECHNOLOGY CORP LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] 1. The research on matsutake mushrooms mainly focuses on mycelium culture and polysaccharide research, while the research on other active ingredients produced by matsutake mushroom mycelium fermentation is less;
[0009] 2. There are very few studies on ergothioneine, a rare amino acid contained in the fruiting bodies and mycelia of matsutake mushrooms;
[0010] 3. No comprehensive utilization of ergothioneine and matsutake polysaccharide in the existing research

Method used

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  • Tricholoma matsutake and method for producing ergothioneine with same
  • Tricholoma matsutake and method for producing ergothioneine with same
  • Tricholoma matsutake and method for producing ergothioneine with same

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specific Embodiment approach

[0052] As a specific embodiment of the present invention, Conduoma Matsutake (PTCC NO: M 2020587) is rapidly grown in the potato gulitin agar medium (ie, PDA solid medium) containing pine extract. Under 25 ° C for 20 days, the colony diameters reached 20 to 25 mm, which was white, colony surface gas mycella, forming a glied line velvet; its colony morphology figure 1 . The strain is composed of tubular cells, thin, with obvious cross, hollow, see figure 2 .

[0053] Conducker is a fungus burr, a bacterium Tricholoma matsutake (S.ito.timai.) Sing, born under pine forest, barefriend like antler, so many famous pine, Song Dynasty Chen Renyu, "Mysteria Spectrum" This bacteria is called Canto. Among them, Condoroma Matsutake CCTCC NO: M 2020587 has been deposited in China's typical cultured center (CCTCC) in China on October 16, 2020.

[0054] The 18s rRNA gene sequence of this Clay CCTCC NO: M 2020587 is SEQ ID NO: 1, see image 3 .

[0055] The Tricholoma Matsutake SR-LY CCTCC NO: ...

Embodiment 1

[0115] Example 1 Screening of Maihape Sulfousia

[0116] (1) The internal tissue block of about 0.5 × 0.5 cm is cut at a pneumatic body cap of different origins, and placed on the PDA solid medium containing antibiotic and rock leaching solution, and the simultaneous paste will be transferred. Purification of PDA solid medium contacted to the tone leaching solution was purified, purified after the purified bacterium was inoculated on the PDA inclined surface of the toner extract, and was stored at 3 to 4 ° C.

[0117] (2) The solid strain obtained by step (1) is inoculated into the fermentation medium, and 30 days at 24 ° C, resulting in a bacterial fermentationery, a fermentation medium, a glucose, 25.0 g / l Corn pulping, 1.0 g / L of hydrogen phosphate, 0.003 g / L vitamin B 6 The rest is water. The precursor cysteine, methionine, and betaine alkali were supplemented on day 20, the concentration was 5 mm, respectively.

[0118] (3) After the end of fermentation, the femoral fer...

Embodiment 2

[0119] Example 2 Morphological observation of Tricholoma matsutake SR-LY

[0120] The CCTCC NO: MATSUTAKE CCTCC NO: MATSUTAKE CCTCC NO: M 2020587 is growing faster in potato gulitin agar medium (i.e., PDA solid medium) containing toner leaching solution, at 25 ° C for 20 days, the colony diameters reached 20 to 25 mm, It is white, colony surface gas bacteria toggle, forming a gliped line velvet; its colony morphology figure 1 . The strain is composed of tubular cells, thin, with obvious cross, hollow, see figure 2 .

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Abstract

The invention provides tricholoma matsutake which has a preservation number being CCTCC No: M 2020587, and a method for producing ergothioneine by using the same. The method for screening tricholoma matsutake strains is simple in process, easy and convenient to operate and low in cost, can be used for screening strains in large scale and easily screening out high-yield strains. The method for biosynthesizing ergothioneine by combined fermentation of tricholoma matsutake and hericium erinaceus is simple in process, enables active ingredients including polysaccharides, amino acids and the like in tricholoma matsutake fermentation liquor can be reserved, and can increase the yield of ergothioneine.

Description

Technical field [0001] The present invention relates to the field of biochemical techniques, and in particular, to a Crane and a method for producing wheat angles. Background technique [0002] Wheat alone (Ergothioneine; 2-Mercaptohistidine Trimethylbetaine) is a rare amino acid with an antioxidant, ultraviolet protection and cell repair. Wheat angiobulfur is present in many animal plants, cannot be synthesized by the animal body, and can only be taken from the food. There are three methods for preparing wheat angles: chemical synthesis, extraction methods, microbial fermentation. Because of the presence of high and unsneas, the microbial fermentation method is more concerned about the preparation of the microbial fermentation. [0003] Conducker, also known as Musaki, famous Songskou mushroom, alphan pine, gentleman, Taichi, belong to the burr, mouth mushroom, is a bacteria, a bacteria, such as pesto, and a unique richness, it is the world. The rare nature of natural medicine, ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01H15/00C12N1/02C12P13/04C12P19/04C12P39/00C12R1/645
CPCC12N1/02A01H15/00C12P13/04C12P39/00C12P19/04
Inventor 魏玉洁陆震李杰陈清平孙元军马良陈雯雯郭文逸郭学平
Owner BLOOMAGE BIOTECHNOLOGY CORP LTD
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