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LC-MS/MS detection method and pretreatment kit for catecholamine

A LC-MS and catecholamine technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of poor pretreatment or treatment effect of catecholamine detection samples, large error in measurement results, and insufficient detection accuracy, etc., and achieve simple recovery The effect of high efficiency, guaranteed stability, and strong separation energy

Inactive Publication Date: 2021-03-16
青岛惠安康生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the lack of pretreatment or poor treatment effect on the test samples, the existing catecholamine detection methods easily lead to matrix effects, resulting in large errors in measurement results and insufficient detection accuracy.

Method used

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  • LC-MS/MS detection method and pretreatment kit for catecholamine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The LC-MS / MS detection method of catecholamines in this embodiment mainly includes the following steps:

[0024] Step 1: Take blank plasma from healthy people and put it in a centrifuge tube, add internal standard solution and diluent to dilute;

[0025] Step 2: Add the diluted solution to the WCX solid-phase extraction column for extraction, and rinse with eluent after extraction;

[0026] Step 3: After rinsing, vacuum dry the WCX solid-phase extraction cartridge, extract the solution and use methyl formate solution to elute, and collect the eluate, and reconstitute the eluate after drying with rare gas;

[0027] Step 4: Take the reconstituted solution and filter it through an ultrafine filter membrane, then place it in a chromatographic column for chromatographic analysis.

[0028] In step 1, the internal standard solution is specifically an internal standard solution of isoproterenol, and the diluted solution is specifically a mixed solution of ascorbic acid and amm...

Embodiment 2

[0034] The LC-MS / MS detection method of catecholamines in this embodiment mainly includes the following steps:

[0035] Step 1: Take the blank plasma and standard samples from healthy people and place them in a centrifuge tube, add internal standard solution and diluent to dilute;

[0036] Step 2: Add the diluted solution to the WCX solid-phase extraction column for extraction, and rinse with eluent after extraction;

[0037] Step 3: After rinsing, vacuum dry the WCX solid-phase extraction cartridge, extract the solution and use methyl formate solution to elute, and collect the eluate, and reconstitute the eluate after drying with rare gas;

[0038] Step 4: Take the reconstituted solution and filter it through an ultrafine filter membrane, then place it in a chromatographic column for chromatographic analysis.

[0039] In step 1, the internal standard solution is specifically an internal standard solution of isoproterenol, and the diluted solution is specifically a mixed solu...

Embodiment 3

[0045] The LC-MS / MS detection method of catecholamines in this embodiment mainly includes the following steps:

[0046]Step 1: Take the patient's plasma and place it in a centrifuge tube, add internal standard solution and diluent to dilute;

[0047] Step 2: Add the diluted solution to the WCX solid-phase extraction column for extraction, and rinse with eluent after extraction;

[0048] Step 3: After rinsing, vacuum dry the WCX solid-phase extraction cartridge, extract the solution and use methyl formate solution to elute, and collect the eluate, and reconstitute the eluate after drying with rare gas;

[0049] Step 4: Take the reconstituted solution and filter it through an ultrafine filter membrane, then place it in a chromatographic column for chromatographic analysis.

[0050] In step 1, the internal standard solution is specifically an internal standard solution of isoproterenol, and the diluted solution is specifically a mixed solution of ascorbic acid and ammonium aceta...

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Abstract

The invention relates to the technical field of catecholamine detection, in particular to an LC-MS / MS detection method of catecholamine, which mainly comprises the following steps: putting a detectionsample into a centrifuge tube, and adding an internal standard solution and a diluent for dilution; adding the diluted solution into a WCX solid-phase extraction column for extraction, and leaching with leacheate after extraction; after leaching, vacuumizing the WCX solid-phase extraction column, pumping out the solution, eluting with a methyl formate solution, collecting eluent, blow-drying theeluent with rare gas, and redissolving; filtering the redissolved solution through an ultrafine filter membrane, and performing chromatographic analysis in a chromatographic column. The invention alsodiscloses an LC-MS / MS detection pretreatment kit for catecholamine. The pretreatment kit comprises a WCX solid-phase treatment extraction column, a diluent and an eluent. According to the invention,the detection precision of catecholamine in the prior art is effectively improved and optimized, and the detection efficiency of the detection process is improved.

Description

technical field [0001] The invention relates to the technical field of catecholamine detection, in particular to an LC-MS / MS detection method and a pretreatment kit for catecholamines. Background technique [0002] Catecholamines are neurological substances containing catechol and amine groups. Catechol and amine groups are bound by an enzymatic step of L-tyrosine in the sympathetic nerve, adrenal medulla and chromaffin cells. Typically, catecholamines refer to norepinephrine (NA), epinephrine (Adr) and dopamine (DA). These three catecholamines are all converted from tyrosine as a precursor. That is, amine compounds containing catechol (ie catechol), including dopamine, norepinephrine, epinephrine and their derivatives. Norepinephrine and epinephrine are both hormones secreted by the adrenal medulla and neuromediators of noradrenergic fibers in the sympathetic and central nervous systems. Noradrenaline is widely distributed in the central nervous system, and its content ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14G01N2030/062G01N2030/045G01N2030/146
Inventor 黄宁刘晓刘传兴
Owner 青岛惠安康生物工程有限公司
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