Brain organoid in-vitro culture chip

Abstract

The invention discloses a brain organoid in-vitro culture chip. The brain organoid in-vitro culture chip comprises an upper-layer chip, a lower-layer chip and a cover plate, the upper-layer chip is asolid material substrate, a liquid inlet, culture hole arrays and a liquid outlet are sequentially formed in a surface of the upper-layer chip, a micropore structure is arranged at a bottom of each culture hole, and a culture pool is formed by the micropore structures and the culture holes; the lower-layer chip is a solid material substrate, a groove is formed in a surface of the lower-layer chip,a circular hole is formed in the groove and extends out of a first channel in a long edge direction of the chip, the first channel is branched into a plurality of branch channels, the branch channelsare converged into a second channel, the circular hole communicates with the liquid inlet, and the branch channels correspond to the hole arrays above; and the cover plate is a solid material substrate and an inlet and an outlet are arranged in a surface of the cover plate. Diameter or the number of the culture hole arrays in the chip can be adjusted, organs are independently cultured in the culture holes, the formed brain organoids have a uniform size and shape, and the brain organoids with the same size can be produced in batches. The brain organoid in-vitro culture chip can avoid generation of large-size brain organoids, reduce a formation of a hypoxia core, and improve a survival rate.

Description

technical field [0001] The invention relates to an organ culture device, in particular to a brain organoid culture chip in vitro. Background technique [0002] Organoids refer to the use of 3D culture technology to cultivate organ-like tissue structures with relatively stable phenotype and genetic characteristics in vitro, which can be used as models and are of great significance in the research of growth and development, physiology and pathology, drug effects, etc. , of which brain organoids are an important branch. Traditionally, rodents are often used for neuroscience research, but the brain tissue structure and development of any rodent are quite different from those of humans. On the other hand, due to ethical and other reasons, it is very difficult to use living brain tissue, so induction The differentiation of pluripotent stem cells to form brain organoids is extremely important. [0003] Brain organoid preparation generally includes four steps, embryonic body forma...

AI Technical Summary

Problems solved by technology

This process is very cumbersome, and the transfer process is extremely prone to contamination or mechanical damage, resulting in loss of embryonic bodies and damage, thereby affecting the yield of brain organoids

In addition, when multiple brain organoids are cultured in the same culture container, different brain organoids are prone to fusion during the formation process, resulting in different sizes and shapes of brain organoids, which have a great impact on subsequent experiments and applications. When the diameter of brain organoids is too large, it is easy to produce hypoxic cores causing necrosis, altering the neural development of gene expression profiles, and reducing the yield of organoids

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