Solid-state fermentation method for improving nutritional value of tea residues
A nutritional value, solid state fermentation technology, applied in the direction of animal feed, animal feed, application, etc., to achieve the effect of improving nutritional value, improving flavor, and increasing content
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Embodiment 1
[0036] The screening of embodiment 1 tea dregs fermentation conditions
[0037] The tea residues used in the following examples are all provided by Master Kong Beverage Co., Ltd., which are tea residues produced after the production of tea beverages. After drying at 60°C, they are ground into powder and passed through a 40-mesh sieve. Trichoderma koningii (T.koningii) used is GenBank: KY764915.1, and the literature Trichoderma koningii isolate CTCCSJ-G-HB40448 small subunitribosomal RNA gene, partial sequence; internal transcribed spacer 1 and 5.8 Sribosomal RNA gene, complete sequence; and The strains in internal transcribed spacer 2, partial sequence are the same. Wherein, the preparation of Trichoderma korningen bacterium liquid is as follows:
[0038] Fungal expansion and preparation of spore liquid: Potato dextrose agar medium (PDA) was used as the expansion medium. 0.2mL 2~3×10 8 pcs / mL Trichoderma korningii spore liquid is applied to the surface of the PDA medium, an...
Embodiment 2
[0100] Embodiment 2 Trichoderma konningen fermentation is on the influence of amino acid type and content in tea dregs
[0101] Amino acid is the basic unit of protein and plays an important role in the body. Its lack or improper ratio will affect the growth and development of animals. Hydrolyzed amino acids are an indicator of amino acid balance and good protein. The improvement of the composition and content of hydrolyzed amino acids is beneficial to increase the nutritional value of tea dregs.
[0102] Free amino acids are a good source of nutrients for animals and can be directly absorbed and used by animals. Therefore, the higher the FAA content, the higher its nutritional value. In addition, free amino acids reflect the sensory flavor of tea dregs, which are the main flavor components and affect the palatability of tea dregs. Therefore, using the type and content of amino acids as an index, the nutritional value changes of tea dregs before and after fermentation by Tri...
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