Aptamer and upconversion nanoparticle modified copolymer as well as synthesis and application thereof

A technology of nanoparticles and copolymers, which is applied in the direction of drug combinations, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve problems such as limitations of in vivo applications, and achieve the effect of avoiding toxic and side effects

Active Publication Date: 2021-04-06
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, the polymerization reaction of carbon-carbon double bonds needs to be catalyzed by an initiator, which is highly toxic and has limitations in in vivo applications.

Method used

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  • Aptamer and upconversion nanoparticle modified copolymer as well as synthesis and application thereof
  • Aptamer and upconversion nanoparticle modified copolymer as well as synthesis and application thereof
  • Aptamer and upconversion nanoparticle modified copolymer as well as synthesis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Preparation of Aptamer and upconversion nanoparticles modified poly(2-hydroxyethyl methacrylate)-cinnamic acid graft copolymer

[0032] 1. Synthesis and amination modification of upconversion nanoparticle UCNP: using rare earth chloride as raw material, upconversion nanoparticle (UCNP) was prepared by solvothermal method: 1 mmol of rare earth chloride salt RECl3 6H2O (RE=79.5%Y, 20% Yb, 0.5% Tm), 15ml octadecene and 6ml oleic acid (OA) were placed in a 100ml three-necked flask, and under the protection of argon, the temperature was raised to 160°C and kept for 40min to ensure that the solid matter was completely dissolved. Then the temperature of the system was naturally cooled to room temperature, and 10 mL of anhydrous methanol mixture dissolved with 0.15 g of ammonium fluoride and 0.1 g of sodium hydroxide was slowly added dropwise into a three-necked flask, and reacted at 50 ° C for 30 min under vigorous stirring conditions. Then, under argon protection, ...

Embodiment 2

[0037] Example 2: Preparation of Aptamer and upconversion nanoparticles modified poly(2-hydroxyethyl methacrylate)-cinnamic acid graft copolymer

[0038] 1. Synthesis and amination modification of upconversion nanoparticle UCNP: using rare earth chloride as raw material, upconversion nanoparticle (UCNP) was prepared by solvothermal method: 1 mmol of rare earth chloride salt RECl3 6H2O (RE=79.5%Y, 20% Yb, 0.5% Tm), 15ml octadecene and 6ml oleic acid (OA) were placed in a 100ml three-necked flask, and under the protection of argon, the temperature was raised to 160°C and kept for 40min to ensure that the solid matter was completely dissolved. Then the temperature of the system was naturally cooled to room temperature, and 10 mL of anhydrous methanol mixture dissolved with 0.15 g of ammonium fluoride and 0.1 g of sodium hydroxide was slowly added dropwise into a three-necked flask, and reacted at 50 ° C for 30 min under vigorous stirring conditions. Then, under argon protection, ...

Embodiment 3

[0043] Example 3: Preparation of Aptamer and upconversion nanoparticles modified poly(2-hydroxyethyl methacrylate)-cinnamic acid graft copolymer

[0044]1. Synthesis and amination modification of upconversion nanoparticle UCNP: using rare earth chloride as raw material, upconversion nanoparticle (UCNP) was prepared by solvothermal method: 1 mmol of rare earth chloride salt RECl3 6H2O (RE=79.5%Y, 20% Yb, 0.5% Tm), 15ml octadecene and 6ml oleic acid (OA) were placed in a 100ml three-necked flask, and under the protection of argon, the temperature was raised to 160°C and kept for 40min to ensure that the solid matter was completely dissolved. Then the temperature of the system was naturally cooled to room temperature, and 10 mL of anhydrous methanol mixture dissolved with 0.15 g of ammonium fluoride and 0.1 g of sodium hydroxide was slowly added dropwise into a three-necked flask, and reacted at 50 ° C for 30 min under vigorous stirring conditions. Then, under argon protection, t...

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Abstract

The invention provides an Aptamer and upconversion nanoparticle modified copolymer as well as synthesis and application thereof. The Aptamer and up-conversion nanoparticle modified copolymer mainly comprises Aptamer, up-conversion nanoparticles and a poly(2-hydroxyethylmethacrylate)-cinnamic acid copolymer, and is prepared by the following steps: dissolving the poly(2-hydroxyethylmethacrylate)-cinnamic acid copolymer in DMSO, adding aminated up-conversion nanoparticles uniformly dispersed in DMSO to prepare the up-conversion nanoparticle modified copolymer. Polyethylene glycol with two ends modified by maleimide and Aptamer are dissolved in a TE buffer solution, and then the up-conversion nanoparticle modified copolymer is added to prepare the target product. The Aptamer and upconversion nanoparticle modified copolymer has no participation of small molecular cytotoxic drugs, can induce aggregation of tumor cell surface receptors under near-infrared irradiation, triggers an apoptosis signal path, and has good anti-tumor curative effects in vivo and in vitro.

Description

technical field [0001] The invention belongs to compounds and synthesis methods, and relates to a copolymer modified by Aptamer and up-conversion nanoparticles and a synthesis method, in particular to Aptamer and up-conversion nanoparticles modified poly(2-hydroxyethyl methacrylate)-cinnamic acid grafting Copolymer and its synthesis method, and the application of Aptamer and up-conversion nanoparticle modified copolymer in the preparation of antitumor drugs. Background technique [0002] Cancer has become the primary problem that seriously threatens health. The global cancer death rate accounts for nearly one-sixth of the total death toll. It has surpassed the incidence of cardiovascular and cerebrovascular diseases and has become one of the main causes of death in the world. At present, the means of clinical tumor treatment mainly include surgical treatment, radiotherapy, chemotherapy, and biological immunotherapy. In addition, some adjuvant tumor treatment methods such as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G83/00A61K41/00A61K47/54A61K47/58A61K47/60A61P35/00
CPCA61K41/0023A61K47/549A61K47/58A61K47/60A61P35/00C08G83/001
Inventor 杜永忠王君徐晓玲应晓英
Owner ZHEJIANG UNIV
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