Nucleotide sequence for encoding novel coronavirus antigen and application of nucleotide sequence

Abstract

The invention provides a nucleotide sequence for encoding a novel coronavirus antigen and application of the nucleotide sequence. According to the nucleotide sequence, a wild-type DNA sequence for encoding SARS-CoV-2 virus surface protein Spike is optimized, a wild-type gene signal peptide is optimized and then inserted into an eukaryotic expression vector, the eukaryotic expression vector is introduced into a host cell, a virus Spike antigen is efficiently expressed in the host cell, and an antiviral humoral immune response and a cellular immune response are systematically activated after antigen presentation. The antibody generated by the activated humoral immune response can prevent invasion of the SARS-CoV-2 virus, and the activated cellular immune response can further remove cells infected by the virus and adjust adverse reactions caused by potential side effects of ADE.

Description

technical field

[0001] The present invention relates to the technical field of vaccines, in particular to a nucleotide sequence encoding a novel coronavirus antigen and its application, specifically in the preparation of vaccines. Background technique

[0002] Coronavirus (Coronaviruses) is an enveloped non-segment positive RNA virus, belonging to Coronaviridae (Coronaviridae) and non-segment virus order (Nidovirales), and is currently the largest known positive-strand RNA virus. According to the serological and genomic characteristics of the virus, the coronavirus subfamily can be divided into four genera: α, β, γ, and δ, among which β-coronaviruses are further divided into four lines: A, B, C, and D. The new coronavirus SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2) belongs to the β genus of coronavirus, mainly transmitted through respiratory droplets, and can also cause pneumonia (Novel Coronavirus-infected Pneumonia, NCP) through contact transmission, and t...

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