Rapid and portable fluorescent quantitative PCR detection device and method

Abstract

The invention discloses a rapid and portable fluorescent quantitative PCR detection device and method. The device comprises a PCR reaction tube and a bifurcated optical fiber, wherein the bifurcated optical fiber comprises an optical fiber bundle end and an optical fiber branch end; the optical fiber bundle end comprises a central laser path, a light source excitation path and a fluorescence detection path; and a first light source excitation device, a second light source excitation device and a fluorescence detection device are arranged at the optical fiber branch end. The method comprises the following steps of adding a sample reaction solution into the PCR reaction tube, and sealing the PCR reaction tube with a sealing film; inserting the optical fiber bundle end into the PCR reaction tube through the sealing film; and irradiating a photo-thermal heating column by laser with different energy, irradiating a sample solution by the light source excitation path, and collecting fluorescence generated by the sample solution by an optical sensor. Rapid and portable detection of a trace sample is achieved, and good application prospects are achieved in the fields of molecular diagnosis, precision medicine, in-vitro detection and in-time diagnosis.

Description

technical field [0001] The invention belongs to the technical field of PCR detection, and in particular relates to a fast and portable fluorescent quantitative PCR detection device and detection method. Background technique [0002] Polymerase Chain Reaction (PCR), as a core technology for in vitro amplification and quantitative detection of nucleic acids in the field of molecular biology, has been widely used in many fields such as in vitro diagnosis, precision medicine, and prevention and treatment of infectious diseases. Therefore, PCR technology has greatly promoted the progress and development of life science and clinical medicine. [0003] With the innovation of PCR detection technology, the second-generation PCR detection technology---fluorescence quantitative PCR technology has been widely developed, and its representative detection devices mainly include QuantStudio analysis system of ThermoFisher Scientific Company and CFX96 analysis system of Bio-Rad Company. Sys...

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Problems solved by technology

However, since its temperature control module usually adopts a metal heating-cooling mode with integrated semiconductor heating elements or air thermal cycle, it often takes a long time to consume a long reaction time after going through dozens of cycles of denaturation-annealing-extension PCR amplification process. Insufficient detection efficiency

[0005] In addition, the larger temperature control module is not conducive to the miniaturization and portability of the detection device

Moreover, the optical detection system of the existing fluorescent quantitative PCR detection device often requires a variety of lenses to cooperate with each other, the optical path design is complicated, and the optical path distance is long, resulting in high optical loss and reduced signal-to-noise ratio, and the fluorescence detection system is huge.

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