Bifid yeast fermentation composition and preparation method thereof
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A fermented composition and a technology for bifidobacteria, which are applied in the field of preparation of bifidus yeast fermentation compositions, can solve the problems of unstable physical and chemical properties of products, low content of small molecular polypeptides, microbial contamination, etc. The effect of improving the quality and stabilizing the fermentation process
Inactive Publication Date: 2021-04-20
天津强微特生物科技有限公司
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[0004] In order to overcome the microbial contamination caused by miscellaneous bacteria in the preparation process of conventional bifid yeast fermentation products, the unstable physical and chemical properties of the product caused by the limitation of bifidobacteria microaerobic fermentation level, and the low content of protein and small molecule peptides that are easy to absorb by the skin, etc.
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Embodiment 1
[0036] A bifid yeast fermentation composition based on Bifidobacterium longum anaerobic fermentation and a preparation method thereof, comprising the steps of:
[0037] (1) Inoculate Bifidobacterium longum into a sterile culture test tube containing 2 mL of MRS medium, anaerobically activate it at 37°C for 24 hours, the initial pH of the medium is 6.0, and add 50% glycerol to a final concentration of 20%, Freeze for later use;
[0038] (2) Add about 60 mL of culture medium into a sterile beaker, so that the thickness of the culture medium is at least 2 cm, add 300 μL of bacterial liquid, mix well, and pour into the beaker. After the culture medium is solidified, transfer it to a 37°C incubator and culture it for 6 days. The obtained flora is resuspended with an appropriate amount of liquid culture medium and used as the secondary fermentation seed liquid;
[0039] (3) Inoculate the secondary seeds into 1L of anaerobic liquid fermentation medium, seal it and let it stand for a...
Embodiment 2
[0044] A bifid yeast fermentation composition based on Bifidobacterium longum anaerobic fermentation and a preparation method thereof, comprising the steps of:
[0045] (1) Inoculate Bifidobacterium longum into a sterile culture test tube containing 10 mL of MRS medium, anaerobically activate it at 37°C for 48 hours, the initial pH of the medium is 6.0, and add 50% glycerol to a final concentration of 20% , frozen for later use;
[0046] (2) Add 75mL of medium into a sterile beaker, add 2.5mL of bacterial solution, mix well, and pour into the beaker. After the agar layer containing bacteria is solidified, continue to pour about 220mL of medium to make the thickness at least 5cm. After the culture medium is solidified, transfer it to a 37°C incubator and culture it for 7 days. The obtained flora is resuspended with an appropriate amount of liquid culture medium and used as the secondary fermentation seed liquid;
[0047] (3) Inoculate the secondary seeds into 5L anaerobic liq...
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Abstract
The invention relates to a bifid yeast fermentation composition and a preparation method thereof. The fermentation composition is formed by mixing bifid yeast lysis substance mother liquor and filtrate according to a certain ratio. The preparation method comprises the following steps of: S1: carrying out anaerobic culture and activation to prepare a first-level fermentation seed solution; S2: inoculating the first-level fermentation seed solution into a micro-aerobic fermentation solid culture medium to obtain a second-level fermentation seed solution; S3: transferring the second-level fermentation seed solution into the fermentation culture medium to carry out micro-aerobic fermentation; S4: through fermentation broth centrifugation, separating bifid yeast filtrate from bifidobacterium bodies; S5: after the bifidobacterium bodies are resuspended and broken, obtaining the bifid yeast lysis substance mother liquor; and S6: mixing the bifid yeast filtrate with the lysis substance mother liquor to obtain a bifid yeast fermentation product. Since bifidobacterium longum is used for carrying out multilevel anaerobic / micro-aerobic fermentation, the problem of a microorganism pollution influence is restricted, and safety is further improved; indexes, including an oxygen content, are controlled in a step-by-step fermentation process, and each physicochemical index of a final product is stable; and through a compound protease digestion technology, the content of polypeptides under same-scale bifidobacterium fermentation is effectively improved.
Description
technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a preparation method of a bifid yeast fermentation composition. Background technique [0002] Bifida Ferment Lysate is a physiological lysate obtained after fermentation of Bifidobacterium. The main components are cell metabolites, cytoplasmic fragments, polysaccharides, proteins and polypeptides. A large number of in vitro experiments have confirmed that a variety of amino acids, proteins and various molecular media in the fermentation product of Bifida yeast have the effects of regulating and balancing the skin and regulating immune function, and are also important components and nutritional molecules of human skin cells; polysaccharides, Molecules such as vitamins and cytoplasmic metabolites of bifidobacteria have certain bail-out, anti-oxidation, and anti-aging effects, and can prevent damage from external stimuli such as ultraviolet rays and promote the repair...
Claims
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