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Glucoside tetrasaccharide as well as preparation method and application thereof

A technology of tetraglucoside and oligoglucoside, which is applied in the interdisciplinary field of natural product engineering and immunology, and can solve the problems of clinical application of difficult immunotherapy and large toxicity heterogeneity

Pending Publication Date: 2021-04-20
UNIVERSITY OF MACAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the TLRs targeting substances or reagents derived from microorganisms in the existing market are too toxic and heterogeneous to be applied in the clinical application of immunotherapy

Method used

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  • Glucoside tetrasaccharide as well as preparation method and application thereof
  • Glucoside tetrasaccharide as well as preparation method and application thereof
  • Glucoside tetrasaccharide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] This embodiment provides a method for preparing tetraglucoside, which specifically includes the following steps.

[0070] Take 1 g of glucomannan extracted from the natural konjac tuber and completely dissolve it in deionized water with a volume of 100 mL, stir for 2 hours, and then freeze-dry the konjac glucomannan solution to obtain konjac glucomannan freeze-dried powder;

[0071] Next, enzymatic degradation treatment of konjac polysaccharide freeze-dried powder: first, 500 mg of konjac glucomannan freeze-dried powder is fully dissolved in 100 mL of deionized water, 60 mg of cellulase is added, the reaction temperature is 35 ° C, the reaction time is 24 hours, and heated to 100°C to inactivate the enzyme, filter and centrifuge after terminating the reaction, and leave the supernatant (oligosaccharide solution);

[0072] The oligosaccharide solution was further separated and purified by Sephadex G-25 dextran chromatography and Sephadex LH-20 hydroxypropyl dextran gel ...

Embodiment 2

[0075] This embodiment provides a method for preparing tetraglucoside, which specifically includes the following steps.

[0076] Take 1 g of glucomannan extracted from the rhizome of natural bletilla striata and completely dissolve it in deionized water with a volume of 100 mL, stir for 2 hours, and then freeze-dry the bletilla striata glucomannan solution to obtain bletilla striata glucomannan freeze-dried powder;

[0077] Next, enzymatically degrade the freeze-dried powder of Bletilla striata polysaccharide: First, fully dissolve 500 mg of Bletilla striata glucomannan freeze-dried powder in 100 mL of deionized water, add 60 mg of cellulase, the reaction temperature is 35 ° C, the reaction time is 24 hours, and heated to 100°C to inactivate the enzyme, filter and centrifuge after terminating the reaction, and save the supernatant;

[0078] The oligosaccharide solution PA18080900 was collected and the eluate was separated and freeze-dried to obtain glucomantetraose with a pur...

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Abstract

The invention discloses glucoside tetrasaccharide as well as a preparation method and application thereof, and relates to the crossing field of natural product engineering and immunology. The main chain structure of the glucoside tetrasaccharide is D-Man-D-Man-D-Man-D-Glc, and the glucoside tetrasaccharide can specifically activate a Toll-like receptor, so as to mediate immune response, and a new thought is provided for developing a novel, effective and safe Toll-like receptor activator or immunologic adjuvant.

Description

technical field [0001] The present invention relates to the interdisciplinary fields of natural product engineering and immunology, and in particular relates to a tetraglucoside sugar and its preparation method and application. Background technique [0002] Mammals detect and defend against microbial invasion through the innate immune system. Bacteria, whether they are pathogens, symbionts or both, will produce pathogen-associated molecular patterns (PAMPs), which are recognized by various types of pattern-recognition receptors (PRR). When PAMPs are detected by the body, they lead to an inflammatory response, which helps eliminate invading microbes. [0003] Some typical PAMPs are derived from molecules on the bacterial cell wall, such as lipopolysaccharide (LPS), lipoprotein, peptidoglycan and flagellin. Long-term studies have shown that PAMPs associated with bacterial cell walls are detected by Toll-like receptors (TLRs), thereby inducing inflammation. However, the TLRs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H3/06C07H1/06A61P29/00A61P37/02A61K39/39A61P37/04
Inventor 王春明殷小雨张哲
Owner UNIVERSITY OF MACAU
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