Preparation method of mesenchymal stem cells capable of reversing senescence

A mesenchymal stem cell and cell technology, applied in the field of mesenchymal stem cell preparation, to achieve the effect of protecting mechanical damage, high cell activity, and increasing viscosity

Pending Publication Date: 2021-04-20
宏之俊生物科技(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Antiaging properties of hMCS i

Method used

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  • Preparation method of mesenchymal stem cells capable of reversing senescence

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Experimental program
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Embodiment 1

[0018] A preparation method for reversing aging mesenchymal stem cells, comprising the following steps:

[0019] S1 Donor selection and evaluation; conduct physical fitness tests and serological studies on cell providers, and exclude donors with signs and symptoms of infectious diseases.

[0020] S2 Isolate human mesenchymal stem cells; separate tissue blocks from the human body, and extract mesenchymal stem cells from them.

[0021] Proliferation culture of S3 cells in vitro; cells isolated from S2 were made into cell suspension, and proliferated in serum-free medium for 10 days; the components of serum-free medium included 55% fibronectin and laminin by volume percentage 2% growth-promoting factor, 2% enzyme inhibitor, 1% trace element, 40% human platelet lysate; the mass ratio of fibronectin and laminin is 3:1.

[0022] S4 cell evaluation; extract part of the cells cultured in S3 proliferation to test and identify cell characteristics, activity, purity, potential, expansio...

Embodiment 2

[0032] A preparation method for reversing aging mesenchymal stem cells, comprising the following steps:

[0033] S1 Donor selection and evaluation; conduct physical fitness tests and serological studies on cell providers, and exclude donors with signs and symptoms of infectious diseases.

[0034] S2 Isolate human mesenchymal stem cells; separate tissue blocks from the human body, and extract mesenchymal stem cells from them.

[0035] Proliferation culture of S3 cells in vitro; cells isolated from S2 were made into cell suspension, and proliferated in serum-free medium for 10 days; the components of serum-free medium included 70% fibronectin and laminin by volume percentage 5% growth-promoting factor, 5% enzyme inhibitor, 3% trace element, 17% human platelet lysate; the mass ratio of fibronectin and laminin is 3:1.

[0036] S4 cell evaluation; extract part of the cells cultured in S3 proliferation to test and identify cell characteristics, activity, purity, potential, expansio...

Embodiment 3

[0046] A preparation method for reversing aging mesenchymal stem cells, comprising the following steps:

[0047] S1 Donor selection and evaluation; conduct physical fitness tests and serological studies on cell providers, and exclude donors with signs and symptoms of infectious diseases.

[0048]S2 Isolate human mesenchymal stem cells; separate tissue blocks from the human body, and extract mesenchymal stem cells from them.

[0049] Proliferation culture of S3 cells in vitro; the cells isolated from S2 were made into cell suspension, and proliferated in serum-free medium for 10 days; the components of serum-free medium included 65% fibronectin and laminin by volume percentage 3% growth-promoting factor, 4% enzyme inhibitor, 2% trace element, 26% human platelet lysate; the mass ratio of fibronectin and laminin is 3:1.

[0050] S4 cell evaluation; extract part of the cells cultured in S3 proliferation to test and identify cell characteristics, activity, purity, potential, expan...

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Abstract

The invention discloses a preparation method of mesenchymal stem cells capable of reversing senescence. The preparation method comprises the following steps: S1, selecting and evaluating a donor; S2, separating human mesenchymal stem cells; S3, performing in-vitro proliferation culture on the cells; S4, performing cell evaluation; S5, preparing a product; and S6, performing cell cryopreservation. According to the invention, the activity of the cells in a mouse body is higher, the cell death number is smaller, the preparation exclusion is smaller, and the characteristic promotes preparation cells to replace regional aging cells in the mouse body to realize reverse senescence, and can also be used for injection treatment of diseases including diabetes, burns, osteoarthritis, graft-versus-host diseases and the like. A serum-free culture medium is selected for in-vitro proliferation culture of the cells, and a human platelet lysate is added into the serum-free culture medium to improve the viscosity of the culture medium, so that the cells are prevented from mechanical damage, cell modification in the proliferation process is avoided, and potential risks existing in conventional fetal calf serum culture, for example, comorbidity with infection sources and microbial pollutants of people and livestock, and immune response, are avoided.

Description

technical field [0001] The invention relates to the field of cell processing, in particular to a method for preparing mesenchymal stem cells that reverse aging. Background technique [0002] Mesenchymal stem cells are derived from the mesoderm in the early stage of development and belong to pluripotent stem cells. MSCs were originally found in the bone marrow. people's attention. Under specific induction conditions in vivo or in vitro, mesenchymal stem cells can differentiate into fat, bone, cartilage, muscle, tendon, ligament, nerve, liver, cardiac muscle, endothelial and other tissue cells. It has multi-directional differentiation potential and can be used as an ideal seed cell for the repair of tissue and organ damage caused by aging and disease. [0003] Existing mesenchymal stem cells play an important role in modern therapeutic and regenerative medicine due to their regenerative and immunomodulatory properties, as well as their ability to migrate to the exact site of...

Claims

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Application Information

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IPC IPC(8): C12N5/0775A01N1/02
Inventor 朱晗
Owner 宏之俊生物科技(上海)有限公司
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