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Immunohistochemical antigen repair buffer solution and use method thereof

A technique of immunohistochemistry and buffer solution, which is applied in biological tests, material inspection products, etc., can solve the problems of volatile repair solution, affect the effect of immunostaining, and small size, and achieve the effect of improving the effect of immunostaining.

Inactive Publication Date: 2021-04-20
深圳市圣通生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to its own structural characteristics, the full-automatic staining instrument has a small container for holding the repair solution, and cannot fully immerse the tissue sections in the repair solution like manual repair, and the automatic immunohistochemical staining Antigen retrieval needs to be carried out at a higher temperature (about 100°C), so the repair solution is easy to volatilize, which further reduces the volume of the repair solution, making its repair effect relatively poor
And it also seriously affected the effect of subsequent immunostaining

Method used

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  • Immunohistochemical antigen repair buffer solution and use method thereof
  • Immunohistochemical antigen repair buffer solution and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] An immunohistochemical antigen retrieval buffer, containing 1.35g Tris-Base, 0.45g trans-2-methyl-2-butenedioic acid, 0.8g mannose erythritol, 0.16 g trypsin, 0.20 g aloe extract, 0.12 g water retaining agent, 0.08 g sodium diacetate, 0.4 g surfactant and the rest of distilled water.

[0028] The preparation method of aloe extract comprises the following steps:

[0029] Ⅰ. Clean the aloe vera, then dry it in the air, peel it, kill it, dry it, crush it, and sieve it. Extract the dry aloe vera powder with water at 40°C for 2 hours. After the extraction, extract the obtained extract After centrifugation in a high-speed centrifuge for 10 minutes, the resulting supernatant is filtered through a filter cloth;

[0030] Ⅱ. Concentrate the filtrate obtained in step I to 1 / 6 of the original volume, then transfer the obtained concentrated solution to ethanol whose volume is 8 times its volume, let it stand for 6 hours at 2°C, and then centrifuge at a high speed Centrifuge for 5 ...

Embodiment 2

[0045]An immunohistochemical antigen retrieval buffer, containing 1.5g Tris-Base, 0.5g trans-2-methyl-2-butenedioic acid, 1.0g mannose erythritol, 0.18 g trypsin, 0.22g aloe extract, 0.14g water retaining agent, 0.10g sodium diacetate, 0.5g surfactant and the rest of distilled water.

[0046] The preparation method of aloe extract comprises the following steps:

[0047] Ⅰ. Clean the aloe vera, then dry it in the air, peel it, kill it, dry it, crush it, and sieve it. Extract the obtained aloe vera dry powder with water at 70°C for 3 hours. After the extraction, extract the obtained extract After centrifugation in a high-speed centrifuge for 15 minutes, the resulting supernatant is filtered through a filter cloth;

[0048] Ⅱ. Concentrate the filtrate obtained in step I to 1 / 4 of the original volume, then transfer the obtained concentrated solution to ethanol whose volume is 9 times its volume, let it stand for 8 hours at 3°C, and then centrifuge at a high speed Centrifuge for ...

Embodiment 3

[0063] An immunohistochemical antigen retrieval buffer, containing 1.62g Tris-Base, 0.55g trans-2-methyl-2-butenedioic acid, 1.2g mannose erythritol, 0.20 g trypsin, 0.25g aloe extract, 0.16g water retaining agent, 0.12g sodium diacetate, 0.6g surfactant and the rest of distilled water.

[0064] The preparation method of aloe extract comprises the following steps:

[0065] Ⅰ. Clean the aloe vera, then dry it in the air, peel it, kill it, dry it, crush it, and sieve it. Extract the dry aloe vera powder with water at 100°C for 4 hours. After the extraction, extract the obtained extract After being centrifuged in a high-speed centrifuge for 20 minutes, the resulting supernatant was filtered through a filter cloth;

[0066] Ⅱ. Concentrate the filtrate obtained in step I to 1 / 3 of the original volume, then transfer the obtained concentrated solution to ethanol whose volume is 10 times its volume, let it stand for 10 hours at 5°C, and then centrifuge at a high speed Centrifuge for...

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Abstract

The invention relates to the technical field of veterinary drug preparation, in particular to an immunohistochemical antigen repair buffer solution and a use method thereof. Each liter of the antigen repair buffer solution contains 1.35 to 1.62 g of Tris-Base, 0.45 to 0.55 g of trans-2-methyl-2-butenedioic acid, 0.8 to 1.2 g of mannosylerythritol, 0.16 to 0.20 g of trypsin, 0.20 to 0.25 g of an aloe extract, 0.12 to 0.16 g of a water-retaining agent, 0.08 to 0.12 g of sodium diacetate, 0.4 to 0.6 g of a surfactant and the balance of distilled water. According to the antigen repair buffer solution provided by the invention, the antigenicity lost in the processes of flaking and the like can be recovered to the greatest extent, so that a good foundation is provided for subsequent antibody staining; the phenomenon that the tissue slice is dried due to excessive volatilization can be effectively prevented, so that the damaged antigen can be smoothly repaired; and besides, cross-linking between proteins caused by aldehyde fixing reagents can be effectively removed, and antigen epitopes in samples such as paraffin sections and the like are fully exposed, so that the immunostaining effect is greatly improved.

Description

technical field [0001] The invention relates to the technical field of antigen retrieval buffer, in particular to an immunohistochemical antigen retrieval buffer and a use method thereof. Background technique [0002] Immunohistochemistry, also known as immunocytochemistry, refers to the qualitative, localization, and quantitative determination of the corresponding antigen by the specific antibody labeled with a chromogenic agent in situ through the antigen-antibody reaction and histochemical color reaction. new technology. It skillfully combines the specificity of the immune response and the visibility of histochemistry, and detects various antigenic substances (such as proteins, peptides, enzymes, hormones, pathogens and receptors, etc.). Immunohistochemical technology has developed rapidly in recent years. [0003] With the application and popularization of automatic immunohistochemical staining instruments, the requirements for staining quality are also increasing. A...

Claims

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Application Information

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IPC IPC(8): G01N33/58
Inventor 张永春
Owner 深圳市圣通生物科技有限公司
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