ABO blood type positive and negative typing and Rh blood type detection card and preparation method thereof
An ABO blood type, positive and negative typing technology, applied in the field of blood typing, can solve the problem of not being able to determine ABO blood type and Rh blood type at the same time, and achieve the effects of reducing the probability of hemolysis risk, prolonging storage time, and improving stability.
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preparation example 1
[0062] Cell Preservative Preparation
[0063] Weigh 1g of sodium chloride, 1g of potassium chloride, 12g of fructose, 1g of lactobionic acid, EDTA-Na 2 9 g, glycine 2.571g, tryptophan 2.571g, valine 0.858g, epigallocatechin 1g, tert-butylhydroquinone 1g, natamycin 18mg, trimethoprim 7mg, antimycin A1 2mg and ATP 0.1 g was dissolved in 1 L of water, and the pH was adjusted to 6.5 to obtain a cell preservation agent.
preparation example 2
[0065] Cell Preservative Preparation
[0066] Weigh 2g of sodium chloride, 1g of potassium chloride, 8g of fructose, 4g of lactobionic acid, EDTA-Na 2 5g, glycine 6.857g, tryptophan 3.429g, valine 1.714g, epigallocatechin 0.5g, tert-butylhydroquinone 1.5g, natamycin 15mg, trimethoprim 9mg, antimycin A1 1mg and 0.15 g of ATP was dissolved in 1 L of water, and the pH was adjusted to 6.8 to obtain a cell preservation agent.
preparation example 3
[0068] Cell Preservative Preparation
[0069] Weigh 1.1g of sodium chloride, 1.1g of potassium chloride, 11g of fructose, 2g of lactobionic acid, EDTA-Na 2 8g, glycine 4g, tryptophan 2.667g, valine 1.333g, epigallocatechin 0.9g, tert-butyl hydroquinone 1.2g, natamycin 17mg, trimethoprim 7.5mg, antimycin A1 1.7mg and ATP 0.12g were dissolved in 1L of water, and the pH was adjusted to 6.6 to obtain a cell preservation agent.
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