ABO blood type positive and negative typing and Rh blood type detection card and preparation method thereof
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An ABO blood type, positive and negative typing technology, applied in the field of blood typing, can solve the problem of not being able to determine ABO blood type and Rh blood type at the same time, and achieve the effects of reducing the probability of hemolysis risk, prolonging storage time, and improving stability.
Pending Publication Date: 2021-04-20
上海润普生物技术有限公司
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Problems solved by technology
[0005] Aiming at the problem that the blood type test card in the prior art cannot determine both ABO blood type and Rh blood type at the same time, th
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preparation example 1
[0062] Cell Preservative Preparation
[0063] Weigh 1g of sodium chloride, 1g of potassium chloride, 12g of fructose, 1g of lactobionic acid, EDTA-Na 2 9 g, glycine 2.571g, tryptophan 2.571g, valine 0.858g, epigallocatechin 1g, tert-butylhydroquinone 1g, natamycin 18mg, trimethoprim 7mg, antimycin A1 2mg and ATP 0.1 g was dissolved in 1 L of water, and the pH was adjusted to 6.5 to obtain a cell preservation agent.
preparation example 2
[0065] Cell Preservative Preparation
[0066] Weigh 2g of sodium chloride, 1g of potassium chloride, 8g of fructose, 4g of lactobionic acid, EDTA-Na 2 5g, glycine 6.857g, tryptophan 3.429g, valine 1.714g, epigallocatechin 0.5g, tert-butylhydroquinone 1.5g, natamycin 15mg, trimethoprim 9mg, antimycin A1 1mg and 0.15 g of ATP was dissolved in 1 L of water, and the pH was adjusted to 6.8 to obtain a cell preservation agent.
preparation example 3
[0068] Cell Preservative Preparation
[0069] Weigh 1.1g of sodium chloride, 1.1g of potassium chloride, 11g of fructose, 2g of lactobionic acid, EDTA-Na 2 8g, glycine 4g, tryptophan 2.667g, valine 1.333g, epigallocatechin 0.9g, tert-butyl hydroquinone 1.2g, natamycin 17mg, trimethoprim 7.5mg, antimycin A1 1.7mg and ATP 0.12g were dissolved in 1L of water, and the pH was adjusted to 6.6 to obtain a cell preservation agent.
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Abstract
The invention discloses an ABO blood type positive and negative typing and Rh blood type detection card and a preparation method thereof, and belongs to the technical field of blood type determination. The blood type detection card comprises eight micro-column gel tubes, an A-type erythrocyte reagent and a B-type erythrocyte reagent, the eight micro-column gel tubes comprise a gel tube containing a monoclonal anti-A antibody with IgM property, a gel tube containing a monoclonal anti-B antibody with IgM property, a gel tube containing a monoclonal anti-D antibody with IgM property, a gel tube containing a monoclonal anti-C antibody with IgM property a gel tube containing a monoclonal anti-E antibody with IgM property, two gel tubes for reverse typing, and a blank gel tube. The blood type detection card can simultaneously detect the ABO blood type and the Rh blood type, can provide an accurate and stable detection result, reduces the hemolysis risk probability in the cross-matching process, and has a long service life.
Description
technical field [0001] The present application relates to the field of blood typing, in particular to an ABO blood type positive and negative typing and Rh blood type detection card and a preparation method thereof. Background technique [0002] The ABO blood group system and the Rh blood group system are the two most important blood group systems for human beings. Their high immunogenicity makes them have important clinical significance. They are not suitable for emergency rescue, surgical treatment, anemia treatment, pre-blood transfusion inspection, etc. What is lacking, the correctness of the test results is directly related to the patient's life safety. [0003] According to the presence or absence of A antigen and / or B antigen on the surface of red blood cell membrane, the blood type is divided into four types: A, B, AB and O. The importance of the Rh blood group system in transfusion medicine is second only to the ABO blood group system. The main antigens of the Rh s...
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