Nanofiber space structure-based controllable bionic tissue engineering scaffold and preparation method

A tissue engineering scaffold, fiber space structure technology, applied in medical science, pharmaceutical formulations, prostheses, etc., can solve the problems of inability to achieve nanofiber structure regulation, affect the mechanical properties of materials, etc., achieve good cell adsorption and promote proliferation ability, Improve mechanical properties and enhance the effect of intermolecular forces

Active Publication Date: 2021-04-23
JIAXING UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In summary, the existing preparation methods of BC porous scaffolds will significantly affect the mechanical properties of the material, and cannot realize the structural regulation of nanofibers in porous scaffolds.

Method used

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  • Nanofiber space structure-based controllable bionic tissue engineering scaffold and preparation method
  • Nanofiber space structure-based controllable bionic tissue engineering scaffold and preparation method
  • Nanofiber space structure-based controllable bionic tissue engineering scaffold and preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0046] A method for preparing a bionic tissue engineering scaffold based on a controllable nanofiber spatial structure, the steps are as follows:

[0047] (1) dissolving gelatin particles (content: 3wt%) and bovine serum albumin (content: 0.2wt%) in deionized water, stirring at 35°C for 1 hour to obtain a gelatin-based aqueous solution;

[0048] (2) Mix the gelatin-based aqueous solution with the protein aqueous solution with a concentration of 1wt%, the mixing volume ratio of the gelatin-based aqueous solution and the protein aqueous solution is 1:0.2, add citric acid after mixing to adjust the pH value of the mixed solution to 5, and then freeze-dry to obtain Composite protein porous scaffold; wherein, freeze-drying is specifically freezing at -196°C for 6 hours, and then vacuum-drying for 12 hours;

[0049] (3) configure the fermentation broth, which is specifically formulated from the following components:

[0050] Glucose 1wt%;

[0051] Peptone 0.05wt%;

[0052] Yeast ...

Embodiment 2

[0068]A method for preparing a bionic tissue engineering scaffold based on a controllable nanofiber spatial structure, the steps are as follows:

[0069] (1) dissolving gelatin particles (content: 1wt%) and bovine serum albumin (content: 0.3wt%) in deionized water, stirring at a temperature of 35° C. for 2 hours to obtain a gelatin-based aqueous solution;

[0070] (2) Mix the gelatin-based aqueous solution with the protein aqueous solution with a concentration of 1wt%, the mixing volume ratio of the gelatin-based aqueous solution and the protein aqueous solution is 1:0.5, add citric acid after mixing to adjust the pH value of the mixed solution to 5, and then freeze-dry to obtain Composite protein porous scaffold; wherein, freeze-drying is specifically freezing at -100°C for 6 hours, and then vacuum-drying for 12 hours;

[0071] (3) configure the fermentation broth, which is specifically formulated from the following components:

[0072] Glucose 2wt%;

[0073] Peptone 0.12wt...

Embodiment 3

[0090] A method for preparing a bionic tissue engineering scaffold based on a controllable nanofiber spatial structure, the steps are as follows:

[0091] (1) dissolving gelatin particles (content: 1.2wt%) and hemoglobin (content: 0.3wt%) in deionized water, stirring at 40°C for 2 hours to obtain a gelatin-based aqueous solution;

[0092] (2) Mix the gelatin-based aqueous solution with the protein aqueous solution with a concentration of 2wt%, the mixing volume ratio of the gelatin-based aqueous solution and the protein aqueous solution is 1:0.8, add citric acid after mixing to adjust the pH value of the mixed solution to 5, and then freeze-dry to obtain Composite protein porous scaffold; wherein, freeze-drying is specifically freezing at -100°C for 12 hours, and then vacuum-drying for 15 hours;

[0093] (3) configure the fermentation broth, which is specifically formulated from the following components:

[0094] Fructose 2.5wt%;

[0095] Peptone 0.2wt%;

[0096] Yeast extr...

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Abstract

The invention relates to a nanofiber space structure-based controllable bionic tissue engineering scaffold and a preparation method. Firstly, a gelatin-based aqueous solution is mixed with a protein aqueous solution and freeze drying is performed after the pH value of the mixed solution is regulated to be 5-7 to obtain a composite protein porous scaffold, then a culture bacteria solution is dropwise added onto the composite protein porous scaffold; until the composite protein porous scaffold adsorbs the culture bacterium solution to be saturated, a fermentation culture solution is added for static culture, and finally a compound obtained through static culture is immersed into a triton aqueous solution with the concentration of 0.1-5wt% for purification and then freeze drying is performed to obtain the bionic tissue engineering scaffold. The prepared bionic tissue engineering scaffold is of a 3D porous structure with pores communicating with each other; and the average pore diameter is 10-300 [mu]m, and the pore diameter range is adjustable. The method according to the invention can control the density distribution of nanofibers in a three-dimensional space, and the prepared bionic tissue engineering scaffold has a good mechanical property, biocompatibility, low endotoxin and good cell adsorption and proliferation promotion capabilities.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and relates to a bionic tissue engineering scaffold based on a controllable nanofiber spatial structure and a preparation method thereof. Background technique [0002] Tissue engineering is the application of the principles and methods of life science and engineering to research and develop clinical substitutes that can maintain, improve or even repair the functions of human tissues (organs), and are used to replace part or all of the functions of tissues (organs). For biological scaffold materials that play a key role in tissue construction, how to design and prepare ideal tissue engineering scaffold materials is still one of the topics of tissue engineering research. Tissue scaffold materials provide the necessary three-dimensional space and mechanical support for the reconstruction of cells and tissues, and play a role in simulating the growth microenvironment of cells in vivo. A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/22A61L27/56
CPCA61L27/222A61L27/227A61L27/20A61L27/56A61L2400/12C08L89/00C08L1/02
Inventor 李喆孙卫华张宝月许志于利超颜志勇李海东生俊露姚勇波吕向国
Owner JIAXING UNIV
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