Device for rapidly detecting number of bacteria in beverage

Abstract

The invention relates to the field of bacteria counting, and discloses a device for rapidly detecting the number of bacteria in a beverage. The device comprises a micro-fluidic chip; the micro-fluidic chip comprises a chip substrate and the following flow paths arranged on the chip substrate, wherein two ends of a sheath flow inflow channel are respectively connected with a sheath flow inlet and a sheath flow fulcrum; two ends of the first sheath flow branch and the second sheath flow branch are respectively connected with the sheath flow fulcrum and the sample focus point; the sample inlet and the sample channel are arranged between the first sheath flow branch and the second sheath flow branch; two ends of the sample channel are respectively connected with the sample inlet and the sample focus point; two ends of the focusing fluid channel are respectively connected with the sample focusing point and the waste liquid outlet; and an optical detection area is arranged in the middle of the focusing fluid channel. The device has high accuracy for bacterial detection, can simplify a flow path and is convenient to clean, so that cross contamination caused by residual bacteria in the flow path is prevented, in addition, detection of a small amount of samples can be realized, the use amount of fluorescent dyes is reduced, and the detection cost is reduced.

Description

technical field [0001] The invention relates to the field of bacteria counting, in particular to a device for quickly detecting the number of bacteria in beverages. Background technique [0002] Flow cytometry is an analytical instrument with flow cytometry as its technical core, which can perform multi-parameter, rapid quantitative analysis and sorting of cells or biological particles in a fast linear flow state in clinical medicine and cell biology. and other fields have important applications. Flow cytometer is mainly divided into four parts: liquid flow system, optical system, detection system, data acquisition and analysis system, and flow cytometer with sorting function also has a sorting system. [0003] The liquid flow system includes a flow chamber and a liquid flow drive system. In the flow chamber, the hydrodynamic focus is realized through the sheath flow technology, and a stable liquid flow of single cell arrangement is formed in the channel, so that the cells ...

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Problems solved by technology

[0004] After using the above-mentioned fluid system to form a stable liquid flow of single cell arrangement, cell counting can be performed, but when this flow cytometer is used to detect the number of bacteria in beverages, the following problems will occur: (1) The liquid flow system is complex, The pipeline is very long, it is very inconvenient to clean, and it is difficult to avoid cross-contamination for samples containing bacteria

The actual detection experiment results show that after the detection of bacteria-containing samples (such as Yakult), more than half an hour of cleaning is required before the bacterial signal of the sample can not be detected. Such a long cleaning time means that flow cytometry is not applicable. It is used for the rapid detection of the number of bacteria in beverages; moreover, bacteria are different from animal and plant cells, the latter cannot reproduce after leaving the appropriate culture medium, pH, temperature and other environments, while the former has a strong reproductive ability, as long as the fluid system has dead ends and is not cleaned Clean, it is easy to breed bacteria, resulting in scrapped flow path

(2) The volume of sample liquid and sheath liquid required for flow cytometry work is too large (because of its long pipeline and large inner diameter), resulting in a large consumption of fluorescent dyes and high experimental costs

(3) Flow cytometry is designed for cell detection, not for bacteria detection. Since the size of bacteria and cells is not on the same order of magnitude, the detection ability of flow cytometry for bacteria is basically close to its lower limit of detection, and there may be multiple When several bacteria are tested together, it is difficult to guarantee the accuracy of the test results

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