Device for rapidly detecting number of bacteria in beverage

A technology for beverages and bacteria, which is applied in the field of devices for rapidly detecting the number of bacteria in beverages. It can solve the problems of scrapped flow paths, inconvenient cleaning, and long inner diameters, and achieve the effects of reducing equipment costs, preventing cross-contamination, and reducing detection costs.
CN112697679APending Publication Date: 2021-04-23HANGZHOU WAHAHA PRECISION MACHINERY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HANGZHOU WAHAHA PRECISION MACHINERY
Publication Date
2021-04-23

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Abstract

The invention relates to the field of bacteria counting, and discloses a device for rapidly detecting the number of bacteria in a beverage. The device comprises a micro-fluidic chip; the micro-fluidic chip comprises a chip substrate and the following flow paths arranged on the chip substrate, wherein two ends of a sheath flow inflow channel are respectively connected with a sheath flow inlet and a sheath flow fulcrum; two ends of the first sheath flow branch and the second sheath flow branch are respectively connected with the sheath flow fulcrum and the sample focus point; the sample inlet and the sample channel are arranged between the first sheath flow branch and the second sheath flow branch; two ends of the sample channel are respectively connected with the sample inlet and the sample focus point; two ends of the focusing fluid channel are respectively connected with the sample focusing point and the waste liquid outlet; and an optical detection area is arranged in the middle of the focusing fluid channel. The device has high accuracy for bacterial detection, can simplify a flow path and is convenient to clean, so that cross contamination caused by residual bacteria in the flow path is prevented, in addition, detection of a small amount of samples can be realized, the use amount of fluorescent dyes is reduced, and the detection cost is reduced.
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Description

technical field

[0001] The invention relates to the field of bacteria counting, in particular to a device for quickly detecting the number of bacteria in beverages. Background technique

[0002] Flow cytometry is an analytical instrument with flow cytometry as its technical core, which can perform multi-parameter, rapid quantitative analysis and sorting of cells or biological particles in a fast linear flow state in clinical medicine and cell biology. and other fields have important applications. Flow cytometer is mainly divided into four parts: liquid flow system, optical system, detection system, data acquisition and analysis system, and flow cytometer with sorting function also has a sorting system.

[0003] The liquid flow system includes a flow chamber and a liquid flow drive system. In the flow chamber, the hydrodynamic focus is realized through the sheath flow technology, and a stable liquid flow of single cell arrangement is formed in the channel, so that the cells ...

Claims

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