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Method for preparing microbial oil from straw resources

A technology for microbial oil and straw resources, applied in the fields of lignocellulose resource conversion and utilization and biorefinery, can solve the problems of low fermentation efficiency and high cost

Active Publication Date: 2021-04-27
WUHAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the above problems, the object of the present invention is to provide a method for preparing microbial oil by using straw resources, aiming to solve the technical problems of low fermentation efficiency and high cost of the existing process

Method used

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  • Method for preparing microbial oil from straw resources
  • Method for preparing microbial oil from straw resources

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] 1) Pretreatment of corn stalks: refer to literature (Yuan, W., et al. Bioresour Technol, 2018, 265:464–470), add 1kg of NaOH to 20L of methanol, fully dissolve and add 10kg of corn stalks passed through a 2mm sieve , fully mixed evenly, and treated at 80°C for 60 minutes, the pretreated straw was washed with methanol three times at a ratio of 1:5, the methanol was evaporated in situ, and the straw was stored for later use.

[0060] 2) Preparation of 50% (w / v) solid-to-liquid ratio corn stalk hydrolyzate: Take 1 kg of pretreated corn stalks and add them to the reactor, mix well, add cellulase at 15 FPU / g, adjust the pH to 4.8, and add water When the total system was 10L, enzymatic hydrolysis was started at 50°C. In the straw storage tank, the straw raw material to be supplemented is evenly sprayed with cellulase solution at an enzyme load of 15 FPU / g, and continuous feeding is started at the 3rd hour of hydrolysis, the feeding rate is 5 g / L / h, and the pH is maintained at...

Embodiment 2

[0065] 1) Pretreatment of wheat straw: refer to literature (Krishnan C, et al. Biotechnol Bioeng. 2010, 107: 441–450), use wheat straw crushed through a 5mm sieve as raw material, add water to form 150% (w / w) The solid-to-liquid ratio was mixed with ammonia water and wheat straw at 2:1 (w / w), treated with ammonia expansion at 140°C for 30 minutes, and cooled to 50°C for later use.

[0066] 2) Preparation of 50% (w / v) solid-to-liquid ratio wheat straw hydrolyzate: take 0.5kg of wheat straw, add 0.16L of the crude enzyme solution collected in Example 1, add appropriate amount of water and mix well, adjust the pH to 4.8, and Add water until the total system is 10L, and start enzymatic hydrolysis at 50°C. In the straw storage tank, the straw raw materials to be added are evenly sprayed with cellulase solution at an enzyme load of 20 FPU / g, and the enzyme-containing straw raw materials are continuously added at a feeding rate of 6.5 g / L / h after the 3rd hour of hydrolysis , until t...

Embodiment 3

[0071] 1) Pretreatment of rice straw: refer to the gas explosion method in the literature (Varga E, et al. Appl Biochem Biotechnol, 2004, 113, 509-523), weigh 10 kg of rice straw passed through a 40-mesh sieve, and perform gas explosion pretreatment.

[0072] 2) Preparation of 50% (w / v) solid-to-liquid ratio rice straw hydrolyzate: take 0.5 kg of straw, add 0.1 L of the crude enzyme solution collected in Example 2, add an appropriate amount of water and mix thoroughly, adjust the pH to 4.8, and Add water until the total system is 10L, and start enzymatic hydrolysis at 50°C. In the straw storage tank, the straw raw materials to be added are evenly sprayed with cellulase solution at an enzyme load of 20 FPU / g, and the enzyme-containing straw raw materials are continuously added at a feeding rate of 6.5 g / L / h after the 3rd hour of hydrolysis , until the solid-liquid ratio reaches 50%, and the enzymatic hydrolysis is completed for 120 hours.

[0073] 3) Directional collection of ...

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Abstract

The invention is applicable to the technical field of lignocellulose resource conversion and utilization and biorefinery, and provides a method for preparing microbial oil by utilizing straw resources, which comprises the following steps: continuously feed-batch supplementing materials to carry out high-solid-phase enzyme hydrolysis on pretreated straws, so that the sugar concentration of the final hydrolysate reaches more than 300g / L; through two times of quantitative water washing, directional preparation of medium-concentration and low-concentration sugar solutions is achieved, the requirement for the performance of filtering or centrifuging equipment in the technological process is lowered, the high-concentration sugar solution, the medium-concentration sugar solution and the low-concentration sugar solution are matched with fed-batch fermentation, and efficient combined utilization is achieved in fed-batch grease fermentation. Besides, soluble oligosaccharides accounting for about 10-20% of the total sugar mass can be generated through high solid phase enzyme hydrolysis, and the oligosaccharides can serve as a strong inducer to induce enzyme-producing fungi to produce a large amount of cellulase for high solid-to-liquid ratio enzyme hydrolysis, so that self-production and self-use of the cellulase are realized, and the enzyme cost is remarkably reduced.

Description

technical field [0001] The invention belongs to the technical field of conversion and utilization of lignocellulose resources and biorefining, and in particular relates to a method for preparing microbial oil by utilizing straw resources. Background technique [0002] With the increasing depletion of coal, oil and natural gas, and the increasingly serious environmental pollution, research on clean and renewable energy such as fuel ethanol and biodiesel has begun to rise. Lignocellulosic biomass is considered to be one of the important raw materials for bioenergy production due to its abundant reserves, low cost, and wide distribution. Among them, crop straw is a typical type of lignocellulosic biomass. Converting straw resources into microbial oil can provide stable and cheap raw materials for the production of biodiesel. To put it simply, the general steps of lignocellulose conversion to microbial oil are: crushing-pretreatment-enzymatic hydrolysis-oil fermentation-oil ext...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12P19/14C12P19/02C12P19/12C12N9/42
CPCC12P7/6463C12P19/14C12P19/02C12P19/12C12N9/2437C12P2201/00C12P2203/00Y02E50/10
Inventor 龚志伟王雪敏周文婷赵觅刘义
Owner WUHAN UNIV OF SCI & TECH
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