Method for preparing microbial oil from straw resources

A technology for microbial oil and straw resources, applied in the fields of lignocellulose resource conversion and utilization and biorefinery, can solve the problems of low fermentation efficiency and high cost

Active Publication Date: 2021-04-27
WUHAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In view of the above problems, the object of the present invention is to provide a method for preparing microbial oil by u

Method used

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  • Method for preparing microbial oil from straw resources
  • Method for preparing microbial oil from straw resources

Examples

Experimental program
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Example Embodiment

[0058]Example 1:

[0059]1) Prerequisite of corn straw: Refer to the literature (YUAN, W., ET Al.Biorell Technol, 2018, 265: 464-470), add 1 kg NaOH to methanol, fully dissolved, add 10 kg over 2mm sieve corn stalk. It is well mixed, 60 min at 80 ° C, and the pretreatment straw is washed 3 times with methanol in the proportion of 1: 5, and the methanol is recovered by methanol, straw storage.

[0060]2) 50% (w / v) solid-liquid than corn stover hydrolyzing solution: Take 1 kg pretreatment corn stalk to add reactor, thoroughly mix, add cellulase according to 15FPU / g, adjust pH to 4.8, and add water The total system was 10 L, and the enzymatic hydrolysis was started at 50 ° C. In the straw storage tank, the straw raw material to be suppressed is uniformly sprayed on the cellulase liquid in the amount of 15fpu / g, and the hydrolysis of the thirdh is started continuously, the filling rate is 5g / l / h, and the pH is maintained. 4.8, the enzymatic hydrolysis 144h ends.

[0061]3) Hydrolyzed ...

Example Embodiment

[0064]Example 2:

[0065]1) Prerequisite of wheat straw: Refer to the literature (Krishnan C, et al.biotechnol Bioeng.2010, 107: 441-450), with wheat straws with sieved 5mm sieve as raw materials, add water into 150% (w / w) The solid-liquid ratio, press 2: 1 (w / w) mixed ammonia water and wheat straw, 140 ° C ammonia expansion treatment for 30 min, cooled to 50 ° C.

[0066]2) 50% (w / v) solid-liquid is prepared from wheat straw hydrolyzing: 0.5kg wheat straw, adding the crude enzyme liquid collected in Example 1, adding appropriate amount of water to sufficiently mixing, adjusting pH to 4.8, and The addition of water to a total of 10 L, and enzymatic solutions were started at 50 ° C. In the straw tank, the amount of cellulase liquid is sprayed in the amount of 20fpu / g of the straw raw material, and the hydrolysis is 6.5 g / l / h continuously to supplement the enzyme straw raw material according to the feed rate of 6.5 g / l / h after hydrolysis. Until the solid solution reaches 50...

Example Embodiment

[0070]Example 3:

[0071]1) Pretreatment of straw straw: referring to the air explosion method of Varga E, ET Al.APPL Biochem Biotechnol, 2004, 113, 509-523, weighs 10 kg of 40 mesh sieve straw, and performs aeronate pretreatment.

[0072]2) 50% (w / v) solid-liquid ratio of straw straw hydrolyzing solution: 0.5 kg straw, adding the crude enzyme solution collected in Example 2, adding the appropriate amount of water, adjusting the pH to 4.8, and The addition of water to a total of 10 L, and enzymatic solutions were started at 50 ° C. In the straw tank, the amount of cellulase liquid is sprayed in the amount of 20fpu / g of the straw raw material, and the hydrolysis is 6.5 g / l / h continuously to supplement the enzyme straw raw material according to the feed rate of 6.5 g / l / h after hydrolysis. Until the solid solution reaches 50%, the enzymatic solution is ended.

[0073]3) Hydrolyzed liquid orientation collection: The supernatant was collected by centrifugation, and the reductant suga...

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Abstract

The invention is applicable to the technical field of lignocellulose resource conversion and utilization and biorefinery, and provides a method for preparing microbial oil by utilizing straw resources, which comprises the following steps: continuously feed-batch supplementing materials to carry out high-solid-phase enzyme hydrolysis on pretreated straws, so that the sugar concentration of the final hydrolysate reaches more than 300g/L; through two times of quantitative water washing, directional preparation of medium-concentration and low-concentration sugar solutions is achieved, the requirement for the performance of filtering or centrifuging equipment in the technological process is lowered, the high-concentration sugar solution, the medium-concentration sugar solution and the low-concentration sugar solution are matched with fed-batch fermentation, and efficient combined utilization is achieved in fed-batch grease fermentation. Besides, soluble oligosaccharides accounting for about 10-20% of the total sugar mass can be generated through high solid phase enzyme hydrolysis, and the oligosaccharides can serve as a strong inducer to induce enzyme-producing fungi to produce a large amount of cellulase for high solid-to-liquid ratio enzyme hydrolysis, so that self-production and self-use of the cellulase are realized, and the enzyme cost is remarkably reduced.

Description

technical field [0001] The invention belongs to the technical field of conversion and utilization of lignocellulose resources and biorefining, and in particular relates to a method for preparing microbial oil by utilizing straw resources. Background technique [0002] With the increasing depletion of coal, oil and natural gas, and the increasingly serious environmental pollution, research on clean and renewable energy such as fuel ethanol and biodiesel has begun to rise. Lignocellulosic biomass is considered to be one of the important raw materials for bioenergy production due to its abundant reserves, low cost, and wide distribution. Among them, crop straw is a typical type of lignocellulosic biomass. Converting straw resources into microbial oil can provide stable and cheap raw materials for the production of biodiesel. To put it simply, the general steps of lignocellulose conversion to microbial oil are: crushing-pretreatment-enzymatic hydrolysis-oil fermentation-oil ext...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12P19/14C12P19/02C12P19/12C12N9/42
CPCC12P7/6463C12P19/14C12P19/02C12P19/12C12N9/2437C12P2201/00C12P2203/00Y02E50/10
Inventor 龚志伟王雪敏周文婷赵觅刘义
Owner WUHAN UNIV OF SCI & TECH
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