Kit for determining glucose content and application thereof

Abstract

The invention relates to the technical field of in-vitro detection, in particular to a kit for determining glucose content and application thereof. The kit comprises an anti-chyle interference reagent, and the anti-chyle interference reagent is selected from one or more of a polyethylene oxide fatty alcohol nonionic surfactant, a polyethylene oxide-propylene oxide fatty alcohol nonionic surfactant, a polyethylene glycol alkyl ether nonionic surfactant and a mixed fatty glyceride nonionic surfactant. On the basis of basic components of the glucose determination kit, 0.01%-10% of one or more specific surfactants are added into a detection reagent, so that the interference of chyle in a lipid blood sample on glucose determination is eliminated, and the accuracy of glucose detection in the lipid blood sample is improved; the reagent is simple in component and easy to produce and prepare; and the cost is low, and commercialization is easy.

Description

technical field [0001] The invention relates to the technical field of in vitro detection, in particular to a kit for measuring glucose content and its application. Background technique [0002] At present, in clinical biochemical testing items, the determination of enzyme activity, organic matter, inorganic ions and trace elements in serum and plasma samples is often used in the diagnosis and auxiliary diagnosis of liver and gallbladder diseases, heart diseases, metabolic diseases, kidney diseases, etc. These The projects are based on Vis-UV spectrophotometry using Lambert-Beer's law as the basis for chemical reaction quantification. [0003] In hyperlipidemia, the blood lipids in the blood rise, especially chylomicrons and very low-density lipoproteins are suspended particles, which make the serum or plasma appear in a certain turbidity or chyle state. These biochemical test items cause a certain degree of interference, resulting in inaccurate measurement results and affe...

AI Technical Summary

Problems solved by technology

This method can reduce the sample blank to a certain extent and improve the accuracy of the measurement, but this method cannot completely eliminate the influence of lipid turbidity on the measurement results, especially after the sample is diluted, the measurement results will appear certain due to the matrix effect. error

2. Ether extraction method: add organic solvent ether to the hyperlipidemic blood sample, shake and mix and then centrifuge, which can effectively extract fat-soluble substances such as triglycerides in the sample, but because this method adds a new chemical Substances that will affect the determination of some test items

3. Precipitation separation method: This method is derived from the determination of HDL by precipitation method, that is, the combined use of phosphotungstic acid-magnesium precipitant and polyethylene glycol-dextran sulfate precipitant to precipitate chylomicrons and very low-density lipoprotein in serum , low-density lipoprotein, and lipoprotein (a), this method will have an impact on the determination of some test items, and the operation is cumbersome, so this method also has certain limitations

However, this method requires high equipment, and small and medium-sized medical institutions do not have high-speed centrifuges, which limits the use of this method.

[0004] Chinese patent CN 107884401 discloses a method for measuring glucose oxidase that eliminates interference from blood lipids. It mainly uses enzymatic reactions to eliminate blood lipids in interference samples. However, this method has many components, complicated preparation, and difficult stability of the reagents. The guarantee and cost are relatively high, and it is difficult to market the product

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