Ldlr negative packaging cell line for the production of vsv-g pseudotyped retroviral vector particles or virus particles thereof
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- ミルテニイビオテックベーファーウントコーカーゲー
- Publication Date
- 2021-04-27
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Abstract
Description
technical field
[0001] The present invention relates to the field of packaging cell lines for the production of retroviral vector particles or virus-like particles thereof, in particular packaging cell lines which do not express low density lipoprotein (LDLR) on their surface, for the production of VSV-G Pseudotyped retroviral vector particles or vector-like particles (VLPs) thereof. Background technique
[0002] In the field of T cell and stem cell gene therapy, retroviral vectors such as lentiviral vectors (LV) are considered to be efficient tools for delivering therapeutic nucleic acid molecules to target cells and inducing long-term expression. Typically, retroviral vectors contain a heterologous envelope protein from a foreign viral species within the retroviral membrane. The process of exchanging viral vector envelope proteins is called "pseudotyping". The most commonly used envelope protein for pseudotyping is the G protein of Vesicular Stomatitis Virus (VSV-G). It...